The effects of training conditions for functional recovery and plasticity after stroke in view of GABA change.

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K19785
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 59010:Rehabilitation science-related
• Research Institution: Hirosaki University
• Principal Investigator: Sato Chihiro 弘前大学, 保健学研究科, 助教 (70757468)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: 脳卒中モデル / 運動麻痺 / リハビリテーション / 脳可塑性 / GABA / KCC2

Outline of Final Research Achievements

Exercise is effective for motor dysfunction after stroke. Elucidation of the mechanism is urgently needed, because it is unknown. We focused on the mechanism by GABA, an inhibitory substance in brain, acts on excitability through changing in KCC2 during development and having injury. In this study, we analyzed the effects of exrcise and changes in KCC2 after stroke using model animals.　As a result, it was found that functional recovery was promoted by exercise, but was greatly affected by exercise style. In addition, KCC2 levels around the lesion decreased markedly after the onset of hemorrhage, and recovered over time.
These results suggested that the prognosis of functional recovery was greatly influenced by the selection of appropriate treatment according to the pathology, and that changing of KCC2 might be involved in the process of functional recovery.

Free Research Field

リハビリテーション

Academic Significance and Societal Importance of the Research Achievements

本研究の結果，脳卒中後に生じた運動機能の回復は運動療法により促進されるが，治療成績は運動様式に大きく左右されることが確認できた．さらに，脳出血巣の周辺組織においては発症直後からKCC2が著しく減少し，経過とともに回復することが明らかとなった．
以上の結果より，病態に応じた適切な治療種目の選択が機能回復の予後を大きく左右すること，機能回復の課程にはKCC2変動を通したGABA作用の転換が脳内ネットワークの再構築に関与する可能性が示唆された．本成果は未だ乏しい脳卒中後リハビリテーションの神経科学的根拠の提言につながるものと考えられる．