2022 Fiscal Year Final Research Report
Creation of a novel functional RNA that converts adenosine to inosine by deamination reaction
| Project/Area Number |
19K20405
|
|---|
| Research Category |
Grant-in-Aid for Early-Career Scientists
|
| Allocation Type | Multi-year Fund |
|---|
| Review Section |
Basic Section 62010:Life, health and medical informatics-related
|
|---|
| Research Institution | Tokyo Metropolitan Institute of Medical Science |
|---|
Principal Investigator |
SUZUKI Yoshinori 公益財団法人東京都医学総合研究所, 脳・神経科学研究分野, 研究員 (80836172)
|
|---|
| Project Period (FY) |
2019-04-01 – 2023-03-31
|
| Keywords | RNA / in vitro選別法 / 塩基編集 / 脱アミノ反応 |
|---|
| Outline of Final Research Achievements |
In this study, we aimed to create a novel RNA sequence that converts adenine (A) to cytosine (I) in RNA sequences. For this purpose, we used in vitro selection (SELEX), which is fishing out sequences with the desired function from random RNA sequences. However, this methodology does not always yield the desired product. Therefore, as a second goal, we also searched for RNA sequences that recognize and bind to specific sites of specific proteins. As a result, we obtained several RNA motifs that are thought to bind specifically to proteins involved in circadian rhythm generation.
|
| Free Research Field |
神経科学、合成生物学
|
| Academic Significance and Societal Importance of the Research Achievements |
本研究の第一の目標であった塩基変換するRNAの作出は、どのようにしてRNAが機能を持つ分子として進化してきたのか？という生命起源の根本に関わるテーマであった。また、近年注目されている一塩基編集技術の発展にも貢献することが期待された。また、第二の目標であるタンパク質検知RNAの作製は、タンパク質の特定機能部位のみを阻害することで、タンパク質の多層的な働きの理解に寄与し、また、新たな治療薬開発にもつながりうるものである。
|
