Development of a sugar tyoe discrimination in protein glycosylation based on protein subcellular localization

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K20410
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 62010:Life, health and medical informatics-related
• Research Institution: Aoyama Gakuin University
• Principal Investigator: Etchuya Kenji 青山学院大学, 理工学部, 助教 (90806499)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: 糖鎖修飾 / バイオインフォマティクス / 細胞内局在

Outline of Final Research Achievements

Glycoproteins with O-glycosylation from Uniprot KB/Swiss-Prot were classified according to signal sequences and O-glycosylations. Datasets were classified into four groups including intracellular, secretory, signal-anchor, and transmembrane proteins based on the signal peptides and transmembrane domains. The distribution of sugar chains differed depending on the intracellular localization, and specific sugar chain modifications were observed in the nucleus, cytoplasm, and mitochondria. This results shown that the O-glycosylation of sugar chains discrimination methods could be applied to developing a highly accurate sugar species discrimination method.

Free Research Field

分子生物学

Academic Significance and Societal Importance of the Research Achievements

細胞内の糖鎖修飾システムを考える上で、タンパク質が翻訳後に修飾を受けることに焦点を合わせたタンパク質の細胞内局在性に寄与する分類と糖種との相関をもとに、タンパク質の細胞内局在に応じた糖鎖修飾分布を明らかにすることは重要である。タンパク質の糖鎖修飾を高精度で判別できる手法の開発により、当該タンパク質がどの糖種を修飾されるのか、高精度で予測できることにより、近年のIT分野の著しい発展から地図アプリにより渋滞が回避できるように、糖種分布マップに沿って細胞内局在シグナルをタンパク質に導入することで、目的の糖種のみを回避するようなタンパク質デザインが可能となる。