Elucidation of onset and malignant transformation mechanism in percutaneous skin malignant tumor, establishment of treatment method

2019 Fiscal Year Final Research Report

• Project/Area Number: 19K20761
• Project/Area Number (Other): 17H06957 (2017-2018)
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund (2019); Single-year Grants (2017-2018)
• Research Field: Dermatology
• Research Institution: Saga University
• Principal Investigator: masuoka miho 佐賀大学, 医学部, 客員研究員 (70588699)
• Project Period (FY): 2017-08-25 – 2020-03-31
• Keywords: 皮膚悪性腫瘍 / 悪性化 / ペリオスチン

Outline of Final Research Achievements

I reconstituted skin tissues in vitro with a 3-dimensional organotypic coculture system using BCC cell lines and fibroblasts. We used WT or Postn knock out fibroblasts in this system to examine the effects of periostin from fibroblasts on BCC cell lines. Periostin did not enhanced infiltration, proliferation and differentiation of BCC cell lines.
Periostin is highly expressed in the skin tissues of morphia type BCC patients. But periostin is not expressed in the skin tissues of nodular type. It is correlated with disease malignancy and infiltration.

Free Research Field

皮膚悪性腫瘍

Academic Significance and Societal Importance of the Research Achievements

これまでの解析において、皮膚組織の免疫染色の結果より、ペリオスチンは悪性度を反映したバイオマーカーであり、EMT(上皮間葉転移)に関与している可能性を考える。EMTは上皮性の腫瘍細胞が、より運動性の高い間葉系細胞の表現型を獲得し、転移を起こしやすくなった状態をいう。したがって、EMTを制御することは悪性化を抑制することであり、新しい癌治療戦略と考えられる。