Linearization of a single DNA molecule using a nanoslit and size measurement using super-resolution imaging method

2019 Fiscal Year Final Research Report

• Project/Area Number: 19K21109
• Project/Area Number (Other): 18H05950 (2018)
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund (2019); Single-year Grants (2018)
• Review Section: 0402:Nano/micro science, applied condensed matter physics, applied physics and engineering, and related fields
• Research Institution: Nagoya University
• Principal Investigator: Azuma Naoki 名古屋大学, 工学研究科, 助教 (50823283)
• Project Period (FY): 2018-08-24 – 2020-03-31
• Keywords: DNAサイズ分析 / マイクロ流体デバイス / 超解像イメージング / DNA１分子操作 / DNA１分子伸長・固定

Outline of Final Research Achievements

In order to quickly prevent the spread of drug-resistant bacteria, it is necessary to quickly and accurately identify the genotype of DNA of the bacteria by size measurement method of DNAs. In the case of conventional methods for size measurement of DNAs, although the number of bacteria was increased by culturing because it required a large number of DNAs for the analysis, but the process of culture took several days. The purpose of this study was to propose a new method of size measurement for a single DNA molecule. Linearization of a single DNA molecule in a microchannel created by using microfabrication technique and accurate size measurement of the DNA using super-resolution imaging method were achieved.

Free Research Field

ナノ計測，バイオ計測，マイクロ・ナノデバイス，ナノトライボロジー

Academic Significance and Societal Importance of the Research Achievements

本研究では，微小流路内におけるDNA１分子の伸長・固定と光学的超解像法による高精度なサイズ測定を実現した．本研究で得られた研究成果は，従来のゲル電気泳動法やマイクロチップ電気泳動法とは原理が全く異なる新しいサイズ分析法を確立するための基盤的な知見となるものである．本分析法によって，分析に多数のDNA分子を必要とせず，DNA１分子でサイズ分析ができるため，細菌の培養が不要となり，分析時間が飛躍的に短縮でき，薬剤耐性菌の迅速な感染対策の実現が期待できる．