2019 Fiscal Year Final Research Report
Development of the new electroporation method for medical applications by electric field mixing technology
Project/Area Number |
19K21119
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Project/Area Number (Other) |
18H05965 (2018)
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Research Category |
Grant-in-Aid for Research Activity Start-up
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Allocation Type | Multi-year Fund (2019) Single-year Grants (2018) |
Review Section |
0403:Biomedical engineering and related fields
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Research Institution | Akita Industrial Technology Center |
Principal Investigator |
OKUBO YOSHINOBU 秋田県産業技術センター, 先進プロセス開発部, 研究員 (30826532)
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Project Period (FY) |
2018-08-24 – 2020-03-31
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Keywords | 電界撹拌技術 / 細胞工学 / 生化学 |
Outline of Final Research Achievements |
Until now, we have been developing electric field mixing technology, which is a technology for stirring droplets three-dimensionally without using a stirrer by using an electric field without using a stirrer. In this study, it was observed that when the present technology was applied to the uptake of a substance into a human cultured cell in a living state, the uptake of the substance was about twice as efficient as the uptake by diffusion. It was suggested that the application of the present technology can significantly improve the uptake efficiency of a substance that can penetrate the cell membrane of human cultured cells even by diffusion.
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Free Research Field |
生化学
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Academic Significance and Societal Importance of the Research Achievements |
本研究において、電界撹拌技術を生きた状態のヒト培養細胞への物質取り込み時に適応したところ、拡散による取り込みに比べ、2倍程度の効率で物質の取り込みが行われることが観察された。ヒト培養細胞において、非接触でかつ化学物質を用いず、さらに細胞にダメージをほとんど与えない物質導入系は画期的なものである。 本研究により確立された手法による取り込み効率向上という結果は、たとえば、ガン細胞への効率的な抗がん剤などの物質導入手法であったり、不妊治療時の卵子への精子遺伝子の導入など、広義の応用を可能とするものである。
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