Development of an artificial metalloenzyme based on a peroxiredoxin from hyperthermophilic archaea

2019 Fiscal Year Final Research Report

• Project/Area Number: 19K21133
• Project/Area Number (Other): 18H05987 (2018)
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund (2019); Single-year Grants (2018)
• Review Section: 0501:Physical chemistry, functional solid state chemistry, organic chemistry, polymers, organic materials, biomolecular chemistry, and related fields
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: Himiyama Tomoki 国立研究開発法人産業技術総合研究所, 生命工学領域, 研究員 (90828310)
• Project Period (FY): 2018-08-24 – 2020-03-31
• Keywords: 人工金属酵素 / 金属錯体 / タンパク質 / ペルオキシレドキシン

Outline of Final Research Achievements

Artificial metalloenzymes (ArMs), in which metal complex catalysts are immobilized on proteins, are paid much attention as the catalysts with high activity and selectivity. In this study, a peroxiredoxin (Prx) from hyperthermophilic archaea, which has high thermal stability and a variety of structures, was utilized as the protein scaffold for an ArM. Besides, the crystal structure of a new Prx exhibiting an interesting assembling behavior was determined, and a genetic method to modify the assembling behavior of a Prx was developed. These results will pave the way for new catalyst design in ArM study.

Free Research Field

生体分子化学

Academic Significance and Societal Importance of the Research Achievements

人工の金属錯体触媒が持つ広い反応適用範囲と、タンパク質で作られる酵素が持つ高い選択性・活性を両立した触媒の開発は、医薬・機能化学品合成やエネルギー化学の観点から重要である。そこで、人工の金属錯体をタンパク質内部に結合して得られる「人工金属酵素」が注目を浴びている。本研究では、特徴的な構造を有し、極めて熱に強いタンパク質である、好熱菌由来ペルオキシレドキシンをタンパク質足場として利用した新規人工金属酵素を開発した。この新しい触媒設計によって、従来困難であった分子変換の実現が期待される。