2019 Fiscal Year Final Research Report
Time-lapse analysis of Muse cell differentiation in vivo
| Project/Area Number |
19K21187
|
|---|
| Project/Area Number (Other) |
18H06062 (2018)
|
|---|
| Research Category |
Grant-in-Aid for Research Activity Start-up
|
| Allocation Type | Multi-year Fund (2019)
Single-year Grants (2018) |
|---|
| Review Section |
0702:Biology at cellular to organismal levels, and related fields
|
|---|
| Research Institution | Tohoku University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2018-08-24 – 2020-03-31
|
| Keywords | Muse細胞 / 多能性幹細胞 / 再生医療 / 分化 / 多光子励起顕微鏡 / in vivo imaging / live imaging |
|---|
| Outline of Final Research Achievements |
Muse cells can be isolated directly from living bodies using the pluripotent stem cell marker SSEA-3 as an indicator. In addition, when administered intravenously to an animal model of the injury, they migrate to the injury site and differentiate into functional cells appropriate to the site. However, their spontaneous differentiation mechanism after transplantation is still unclear.
In this study, we attempted to observe in detail the differentiation of Muse cells after transplantation by using a multi-photon microscope, which enables us to keep cerebral infarction model mice alive for a long time.
The results showed that Muse cells started to differentiate within 1-2 days after transplantation.
|
| Free Research Field |
再生医療
|
| Academic Significance and Societal Importance of the Research Achievements |
Muse 細胞は生体から単離することができ、高い分化能を維持したまま移植に用いることができる多能性幹細胞である。点滴投与すると損傷部位へと優先的に遊走し、さらにその部位に応じた機能的な細胞へと分化することから、将来性が有望視されているが、一方で移植後の自発的な分化メカニズムなどの生体内での詳細な挙動は未だ不明なままである。
本研究の結果は今後Muse細胞を用いた研究を行う際の時間的計画を立てる際の足掛かりとなるだけではなく、事前に誘導をかけているわけでもないMuse細胞が、どのようにして周囲の環境を読み取り、迅速に分化するのか、その詳細なメカニズムを解明する大きな一助となるものと考えられる。
|
