2019 Fiscal Year Final Research Report
New intracellular signal transduction mechanism by plasma membrane associated protein PCaP1
| Project/Area Number |
19K21191
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| Project/Area Number (Other) |
18H06067 (2018)
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Multi-year Fund (2019)
Single-year Grants (2018) |
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| Review Section |
0702:Biology at cellular to organismal levels, and related fields
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Project Period (FY) |
2018-08-24 – 2020-03-31
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| Keywords | Caシグナル / 脂質シグナル / 情報変換分子 |
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| Outline of Final Research Achievements |
PCaP is a unique molecule that converts a Ca signal to a lipid signal. This protein was found in our laboratory and its physiological function has been analyzed by the applicant.
The purpose of this study is to analyze the physiological role and its biochemical mechanism of PCaP, which may be a hub molecule for intracellular signal transduction. First, we analyzed the PCaP KO mutants and its overexpressors using Arabidopsis thaliana and Marchantia polymorpha. Second, I also purified PCaP recombinant protein synthesized in Escherichia coli and analyzed its biochemical properties of the PCaP protein. Analysis of pcap1 KO mutant of Arabidopsis thaliana revealed that PCaP is involved in root hydrotropism. Also, I have started a analysis to take advantage of Marchantia polymorpha, which is the origin of land plant and has a small number of genes. Both the KO and overexpressing lines of M. polymorpha showed clear morphological and growth abnormalities, suggesting the importance of PCaP1.
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| Free Research Field |
植物生理学
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| Academic Significance and Societal Importance of the Research Achievements |
細胞膜に結合しているタンパク質PCaPは、Caシグナルを脂質シグナルに変換する唯一の分子であると想定される。細胞内情報伝達の重要なハブ分子であるPCaPの生化学的作動機構と生理的役割を明らかにすることで、Caシグナルの巾の広さと特異性を支える新たな仕組を解明することができる。
申請者は、PCaPがシロイヌナズナでは根の水分屈性および葉の気孔閉口に関与していることを明らかにし、植物の環境適応に重要な分子であることを明らかにした。
また、PCaPはほとんど全ての植物に保存されている重要な遺伝子であり、植物進化の基部に位置するゼニゴケにおいても不可欠な機能を果してるタンパク質であるとの知見を得た。
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