Development of New Hybrid Treatment for Osteosarcoma Transplanting Controlled-release Pirarubicin Conjugated Adipose-derived Stem Celll

2019 Fiscal Year Final Research Report

• Project/Area Number: 19K21272
• Project/Area Number (Other): 18H06162 (2018)
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund (2019); Single-year Grants (2018)
• Review Section: 0901:Oncology and related fields
• Research Institution: Kobe University
• Principal Investigator: KAWAKAMI YOHEI 神戸大学, 医学部附属病院, 医員 (50626570)
• Project Period (FY): 2018-08-24 – 2020-03-31
• Keywords: 骨肉腫 / 脂肪組織由来幹細胞 / ドラッグデリバリーシステム / 抗がん剤

Outline of Final Research Achievements

The development of new therapeutic strategies for Osteosarcoma (OS) is clinically expected. Adipose-derived stem cell（AdSC）has also the phagocytic capacity and accumulation ability to the tumor site. In order to overcome the problem of low efficacy/frequent side effects by high dose of anti-cancer drug treatment, we utilized poly lactic/glycolic acid (PLGA) microparticles as a tool for slow release drug delivery system. In this study, we demonstrated that Therarubicin conjugated PLGA and AdSC transplantation statistically reduced the tumor proliferation ability via reduction of the tumor fibrosis and hypoxia. Moreover, we also demonstrated the new drug delivery system using AdSCs. This new hybrid treatment of transplanting PLGA (Controlled-release Therarubicin) conjugated AdSC exert as biphasic antitumor potency, firstly reduction of fibrosis and hypoxia of tumors and secondly drug delivery system in order to localize the anticancer drug and reduce of its side effect.

Free Research Field

整形外科学

Academic Significance and Societal Importance of the Research Achievements

腫瘍選択的DDSの開発は次世代のがん治療には欠かせない研究分野である。これまでにAdSCsが炎症部位に集積し抗炎症効果や抗線維化効果を引き起こす事が報告されている。さらにAdSCは貪食能を有し、PLGAナノ粒子を細胞質内に取り込む現象も確認できている。本研究では、多分化能を有し種々の組織再生を目的とした自己細胞移植治療のソースとして注目されているAdSCを用いた徐放化抗がん剤包合細胞移植治療という新しいがん治療法の開発の可能性が示された。他のがん種への応用や様々な薬剤への応用が期待され、今後のがん治療研究分野において革新的な領域を創造することになると考えられた。