Redox biology-based strategy in bone regeneration of iPS cells

2019 Fiscal Year Final Research Report

• Project/Area Number: 19K21362
• Project/Area Number (Other): 18H06271 (2018)
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund (2019); Single-year Grants (2018)
• Review Section: 0907:Oral science and related fields
• Research Institution: Tohoku University
• Principal Investigator: Watanabe Jun 東北大学, 大学病院, 医員 (30822241)
• Project Period (FY): 2018-08-24 – 2020-03-31
• Keywords: iPS細胞 / 酸化ストレス / 骨芽細胞分化 / 骨再生

Outline of Final Research Achievements

Induced pluripotent stem cells (iPSCs) fabricate three-dimensional calcified bone-like constructs in vitro. However, the low efficiency of osteogenic differentiation is a one of the challenge. Recently, reactive oxygen species (ROS) do not only disrupt the intracellular redox balance, resulting in oxidative stress, but also play an important role in stem cell fate.
I applied small molecule antioxidants to induce osteogenic differentiation in mouse iPS cells. These results indicated that the suppression of oxidative stress might promote osteogenic differentiation in iPS cells.

Free Research Field

歯科補綴学

Academic Significance and Societal Importance of the Research Achievements

広範な顎骨欠損を回復する骨再生技術の開発は、歯科補綴学の発展にとって重要な課題の一つである。しかしながら、既存の顎堤増生術の効果は未だ十分ではなく、幹細胞を用いた新規骨再生技術の開発に期待が寄せられている。本研究成果は幹細胞由来の骨再生医療に役立つ可能性を秘めているため社会的に大きな意義がある。また、iPS細胞の骨芽細胞分化とレドックス環境の変化の関連性を示唆する本結果は、幹細胞分化の分子生物学的機序の解明にも繋がるため学術的にも意義が大きいと言える。