Development of platform technology to construct a novel dermal substitute using oral mucosa fibroblasts in hypoxic environment.

2019 Fiscal Year Final Research Report

• Project/Area Number: 19K21378
• Project/Area Number (Other): 18H06290 (2018)
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund (2019); Single-year Grants (2018)
• Review Section: 0907:Oral science and related fields
• Research Institution: Niigata University
• Principal Investigator: Saito Yuko (原夕子) 新潟大学, 医歯学総合病院, 医員 (80827676)
• Project Period (FY): 2018-08-24 – 2020-03-31
• Keywords: 低酸素環境 / 口腔粘膜線維芽細胞 / 3次元培養環境 / 皮膚線維芽細胞 / マイクロアレイ

Outline of Final Research Achievements

Fibroblasts were embedded in collagen gel. Using a microarray, comprehensive gene expression was searched for in 4 groups: oral fibroblasts vs skin fibroblasts, hypoxic cultures vs normal oxygen cultures. The matrix metalloprotease (MMP), the metalloprotease inhibitor (TIMP), etc. in the culture supernatant were measured by a multiplex assay, and the performance of the cultured dermis was evaluated and compared. Type I and III collagen in the culture supernatant was measured by enzyme-linked immunosorbent assay (ELISA). Expression of α-SMA (phenotypic marker of fibroblasts differentiated into myofibroblasts) and CEMIP (cell migration index) in the cultured dermis was measured by Western blotting.

Free Research Field

再生医療

Academic Significance and Societal Importance of the Research Achievements

創傷に対する既存治療法（成長因子投与や人工真皮移植など）では、長期間残存して難治化した傷に奏功せず、効果的治療法の開発は喫緊の課題である。口腔粘膜組織の線維芽細胞は瘢痕形成が皮膚組織に比べわずかとされているが、口腔粘膜線維芽細胞による“培養真皮/結合組織”は開発されていない。本研究の目的である口腔粘膜線維芽細胞の2および3次元培養環境における低酸素応答をタンパクレベルで機能的に解析することや口腔粘膜線維芽細胞と皮膚線維芽細胞の比較検討は、口腔粘膜線維芽細胞を組み込んだ再生医療用製品などの新規培養真皮開発技術基盤構築の第一歩となると考えられる。