Establishment of clinical culture method for human dental pulp stem cells under the xeno-free culture condition.

2019 Fiscal Year Final Research Report

• Project/Area Number: 19K21403
• Project/Area Number (Other): 18H06317 (2018)
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund (2019); Single-year Grants (2018)
• Review Section: 0907:Oral science and related fields
• Research Institution: The Nippon Dental University
• Principal Investigator: Mochizuki Mai 日本歯科大学, 生命歯学部, 助教 (90821934)
• Project Period (FY): 2018-08-24 – 2020-03-31
• Keywords: 無血清培養 / 歯髄幹細胞 / 間葉系幹細胞 / 細胞培養 / 再生医療

Outline of Final Research Achievements

We previously reported that a xenogeneic serum-free culture medium (XFM) is preferable over fetal bovine serum-containing culture medium for ex vivo expansion of human dental pulp-derived stem cells (hDPSCs). However, we observed that cells grown in XFM frequently undergo apoptosis when they form multilayered cell structures upon reaching over-confluence. Therefore, we focused on optimizing the XFM culture system to avoid the undesirable death of hDPSCs.
This study demonstrated that type 1 collagen coating of the cultureware promoted hDPSC adhesion and proliferation. Furthermore, this coating prevented cell death due to multilayered cell growth in XFM conditions. The results provide further insights into the safe and reliable handling of somatic stem cell cultures.

Free Research Field

発生・再生医科学

Academic Significance and Societal Importance of the Research Achievements

本研究は、がん化リスクのないヒト歯髄幹細胞の再生医療応用に向け、細胞治療に有用な培養法の確立を目指し、無血清培養における効果的な歯髄幹細胞培養に貢献する知見を提供した。