Estimation of Selenium Intake in Japan

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K21442
• Project/Area Number (Other): 18H06359 (2018)
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund (2019); Single-year Grants (2018)
• Review Section: 0908:Society medicine, nursing, and related fields
• Research Institution: Osaka Institute of Public Health
• Principal Investigator: Murano Koichi 地方独立行政法人 大阪健康安全基盤研究所, 衛生化学部, 研究員 (50827277)
• Project Period (FY): 2018-08-24 – 2023-03-31
• Keywords: セレン / ICP-MS / トータルダイエット

Outline of Final Research Achievements

To estimate selenium intake in Japan, each group of samples prepared using the total diet method was microwave resolved and then measured for selenium content by inductively coupled plasma-mass spectrometry (ICP-MS). The samples prepared from 2018 to 2008 were Based on the results of the analysis, selenium intake was estimated to be between 106 and 148 ug/day, and it was inferred that there was no significant deviation from the previously considered intake (approximately 100 ug/day).
Liquid chromatography-ICP-MS was used to determine the speciation of selenium compounds. Seleno-L-methionine was identified by protease digestion alone. Multiple peaks were detected when further digestion with reducing and alkylating agents, suggesting that the digested peptides may contain seleno-L-cysteine, which is a protein moiety.

Free Research Field

金属分析

Academic Significance and Societal Importance of the Research Achievements

欧州や米国において、セレン摂取量と生活習慣病との関連性に関する疫学研究や臨床研究が行われている一方で、国内ではそのような研究報告が少ない。本研究は近年における国内のセレン摂取量を推定し、数値として示すことで、各種生活習慣病との関連性を検討するための足掛かりとなる有用な知見となることが考えられる。
また本研究のセレン摂取量の推定結果から、ほとんどの日本人は推奨量以上のセレンを摂取していることが推察され、サプリメント等によるセレンの過剰摂取に注意を促すエビデンスとなることが考えられる。