2019 Fiscal Year Final Research Report
Development of forensic human identification method baced on virome by next generation sequencing
| Project/Area Number |
19K21485
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| Project/Area Number (Other) |
18H06406 (2018)
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Multi-year Fund (2019)
Single-year Grants (2018) |
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| Review Section |
0908:Society medicine, nursing, and related fields
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| Research Institution | National Research Institute of Police Science |
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Principal Investigator |
Kishimto Mai 科学警察研究所, 法科学第一部, 研究員 (20822068)
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| Project Period (FY) |
2018-08-24 – 2020-03-31
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| Keywords | 皮膚常在ウイルス叢 / 個人識別 / メタゲノム解析 / 次世代シークエンサー |
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| Outline of Final Research Achievements |
Forensic science is application for the purpose of criminal investigation and evidence production in court. Here, we investigated human skin virome, including both DNA and RNA viruses to explore the possibility of individual identification. Total 48 samples corrected from four subjects at four body sites over a week and a month were analyzed. In result, 0.85% reads obtained were estimated to be derived from viruses including 78 families, 274 genus. Reads of human infectious papillomaviruses, herpesviruses and other viruses were detected from all samples with high diversity of genera. In conclusion, comprehensive human skin virome were revealed in this study, however, individual identification could not achieved only from skin virome analysis using NGS. Deep-sequencing of PCR amplicon derived from universal primer targeting Papillomaviridae and Herpesviridae, which were human infectious and constantly detected, is thought to be nececcery for individual identification.
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| Free Research Field |
法科学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究は、次世代シークエンサーを用いた細菌・ウイルスを含む網羅的なヒト常在微生物叢解析による個人識別法に関する基礎的検討を行うものである。このような網羅的な常在微生物叢のメタゲノム解析による個人識別により、現在のDNA型鑑定に十分な質・量のヒトDNAが得られない資料の個人識別に役立つ可能性がある。これまでヒト皮膚常在ウイルス叢研究は、二本鎖DNAウイルスのみを対象としたものに限られていたが、今回初めてDNAおよびRNAウイルスを包括したヒト皮膚常在ウイルス叢を明らかにした。その結果、多くのヒト皮膚検体から安定して検出されるウイルス種が明らかになり、個人識別の指標となる可能性が示唆された。
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