Investigation of the rapid culture method using inert solvent

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K22347
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 41:Agricultural economics and rural sociology, agricultural engineering, and related fields
• Research Institution: Kyoto University
• Principal Investigator: Ogawa Yuichi 京都大学, 農学研究科, 准教授 (20373285)
• Co-Investigator(Kenkyū-buntansha): 原田 昌彦 東北大学, 農学研究科, 教授 (70218642) ; 菊池 正二郎 兵庫医科大学, 医学部, 准教授 (70381960)
• Project Period (FY): 2019-06-28 – 2021-03-31
• Keywords: 細菌培養 / 不活性溶剤 / センサ / 代謝物

Outline of Final Research Achievements

As a method for rapidly and quantitatively monitoring the bacterial growth ability, we succeeded in constructing a method for accurately measuring the bacterial growth in the medium using Near-field array sensor. Furthermore, from the image data obtained by the sensor, it was found that bacterial growth flourished at the boundary between perfluorocarbon and the medium. This results suggests that local interactions at the interface affect bacterial growth. As a result of analyzing the composition of the components in the medium by LC-MS / MS, it was clarified that the addition of perfluorocarbon has a significant effect on the amount of metabolites of a specific component. The combination of the developed evaluation system and composition analysis will enable detailed elucidation of the mechanism by which the addition of perfluorocarbons affects bacterial growth potential.

Free Research Field

生物センシング工学

Academic Significance and Societal Importance of the Research Achievements

多くの細菌検査の場面において，従来の検査法では最初に細菌を平板培養法で増殖させる必要がある。そのような中，不活性溶媒を培地に添加すると細菌の増殖能が向上することが分かってきた。しかし，どのような仕組みで不活性溶媒が細胞増殖に影響を与えているかが明らかになっていない。そこで我々は本研究を通じて，迅速かつ定量的な微生物モニタリング技術や代謝物解析からメカニズム解明につながる知見を得た。この結果は，食の安全安心だけでなく，今後ますます重要となる微生物利用の場面でも利用可能な技術であり，食料生産や微生物エネルギー利用，さらには感染症対策や創薬などの場面でも広く貢献できる基盤的な技術と期待される。