Comprehensive identification of polyubiquitinated substrates for elucidation of the "ubiquitin code"

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K22408
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 44:Biology at cellular to organismal levels, and related fields
• Research Institution: Hokkaido University
• Principal Investigator: watanabe masashi 北海道大学, 医学研究院, 講師 (10632424)
• Project Period (FY): 2019-06-28 – 2022-03-31
• Keywords: ユビキチン

Outline of Final Research Achievements

Reversible modification of proteins by ubiquitin is one of the important post-translational modifications that support various life phenomena. In recent years, it has been focused on elucidating the relationship between the diverse polyubiquitin chain and its function via the seven lysine residues and amino-terminal methionine in ubiquitin itself. In this study, we developed several probes that efficiently capture polyubiquitin chains and verified their chain specificity. As a result, we obtained several probes that retain various specificities.

Free Research Field

生化学

Academic Significance and Societal Importance of the Research Achievements

本研究では、ポリユビキチン鎖を効率よく抽出するプローブの開発を行った。この技術をさらに発展させることで、個々のポリユビキチン鎖の効率的な抽出が可能となり、さらに個々のユビキチン付加酵素（ユビキチンリガーゼ）がどの基質に対してどのポリユビキチン鎖を付加しているのかという情報を先入観なく網羅的に同定することが可能となるものと思われる。多彩なポリユビキチン鎖を介した未解明の制御機構の発見に結びつくことが期待できる。