Unraveling biological significance of RNA higher-order structure

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K22519
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 48:Biomedical structure and function and related fields
• Research Institution: The University of Tokyo (2020-2021); Institute of Physical and Chemical Research (2019)
• Principal Investigator: Ariyoshi Tetsuro 東京大学, 大学院医学系研究科(医学部), 客員研究員 (00782103)
• Project Period (FY): 2019-06-28 – 2022-03-31
• Keywords: RNA / 蛍光RNA / RNA高次立体構造

Outline of Final Research Achievements

In this study the person in charge revealed the regulation of RNA higher-order structure by proteins.
First the person in charge developed the screening system for RNA higher-order structure regulating proteins in 293T cells and iPS cells. Then by utilizing AiryScan confocal microscope and fluorescent RNA "Romanesco" the person in charge revealed RNA higher-order structure regulating proteins-dependent fluorescence decrease. By screening some candidate RNA higher-order structure regulating proteins the person in charge successfully revealed that some proteins possess the ability to regulate RNA higher-order structure in cells.

Free Research Field

分子生物学

Academic Significance and Societal Importance of the Research Achievements

RNA立体構造を細胞内で制御するタンパク質の同定により、RNA立体構造の異常に起因すると考えられている疾患の新たな治療法開発の道を開いた。