2020 Fiscal Year Final Research Report
Live imaging of human pancreatic cancer stem cell dynamics
| Project/Area Number |
19K22619
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 53:Organ-based internal medicine and related fields
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| Research Institution | Kyoto University |
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Principal Investigator |
Seno Hiroshi 京都大学, 医学研究科, 教授 (90335266)
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| Project Period (FY) |
2019-06-28 – 2021-03-31
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| Keywords | 膵臓 / 難治がん |
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| Outline of Final Research Achievements |
The aim of this study was to establish in vitro and in vivo experimental systems that mimick human pancreatic cancer development process and to observe cancer "stem" cell behaviors in those systems. We established human pancreatic cancer organoids and inserted DCLK1-CreERT and CMV-LAL-tdTomato constructs to them. In these organoids and subsequently established xenografts, lineage tracing experiments showed that DCLK1-positive pancreatic cancer cells have a potential of cancer "stem" cells. Thus, DCLK1-positive pancreatic cancer cell might be a novel therapeutic target.
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| Free Research Field |
腫瘍生物学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究は、難治がんの代表であるヒト膵がんの発育進展過程を独自の生体内外モデルで可視化する試みである。膵がんのバイオロジーと新規治療法を探索する過程で、「マウスからヒトへ」さらに「ヒトにおける実用化」を視野に入れた知見を獲得し、発展させる可能性を有する。またDCLK1陽性がん細胞は、膵がん以外のヒト消化器がんにひろく存在している。そのため、将来的により普遍性の高い「消化器がん幹細胞標的療法」という新しい治療コンセプトへの道を拓く挑戦性も備えた研究と思われた。
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