2022 Fiscal Year Final Research Report
Establishment of PDOX mouse model for biliary tract cancer using super-microsurgery
| Project/Area Number |
19K22656
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 55:Surgery of the organs maintaining homeostasis and related fields
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| Research Institution | Kanazawa University (2020-2022)
Kyoto University (2019) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
田浦 康二朗 京都大学, 医学研究科, 准教授 (80378629)
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| Project Period (FY) |
2019-06-28 – 2023-03-31
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| Keywords | PDOX / ヌードマウス / 胆管癌 |
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| Outline of Final Research Achievements |
The aim of this study was to establish a mouse model of biliary tract cancer as PDOX. Using microsurgical techniques, patient-derived biliary cancers were transplanted subcutaneously into nude mice. After the tumor grew, the tumor fragments were transplanted into the intrahepatic bile duct (liver) and extrahepatic biliary system (bile ducts and gallbladder) as PDOX by surgical suture fixation. Good engraftment and enlargement were observed within 2 months. In conclusion, the direct transplantation model to the liver (intrahepatic bile duct) was considered to be a simple model with high versatility and reproducibility in terms of technical ease and good engraftment.
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| Free Research Field |
pancreaticobiliary cancer
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| Academic Significance and Societal Importance of the Research Achievements |
本研究での胆道癌PDOXモデルは、胆道癌全般における理想的動物モデルとして用いることで、臨床で有効性が高い新規治療薬・治療法の開発研究が可能となる。また個々の患者においてはその未来を予測する動物モデルとして用いることが可能であり、治療法を事前に試して効果判定を行った後に実際にその患者に行うという真の個別化医療が可能となる。
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