2021 Fiscal Year Final Research Report

Development of an anorexia animal model using an arterially perfused decorticated mouse preparation

Research Project

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Project/Area Number	19K22726
Research Category	Grant-in-Aid for Challenging Research (Exploratory)
Allocation Type	Multi-year Fund
Review Section	Medium-sized Section 57:Oral science and related fields
Research Institution	Showa University
Principal Investigator	Inoue Tomio 昭和大学, 歯学部, 教授 (70184760)
Co-Investigator(Kenkyū-buntansha)	中村史朗昭和大学, 歯学部, 准教授 (60384187) 中山希世美昭和大学, 歯学部, 講師 (00433798) 望月文子昭和大学, 歯学部, 講師 (10453648) 壇辻昌典昭和大学, 歯学部, 助教 (60826634)
Project Period (FY)	2019-06-28 – 2022-03-31
Keywords	食欲 / 動脈灌流標本 / 嚥下
Outline of Final Research Achievements	Anorexia is caused by physical and emotional diseases, as well as aging. It is not clear that neural mechanisms underlying appetite control because the amount of food intake which is used for the the analysis in conventional animal models, is often affected by health condition of the subjects. In this study, we analyzed the effects of several physiologically active substances that have been ascribed to affect the amount of food intake on swallowing-like movement induced in an arterially perfused decerebrated rat preparation to establish a new experimental model to analyze neural mechanisms underlying appetite control. Application of ghrelin increased swallowing-like movement by 31%. Thus, it is possible that the arterially perfused decerebrated rat preparation may be used for analyze neural mechanisms underlying appetite control.
Free Research Field	口腔生理学
Academic Significance and Societal Importance of the Research Achievements	摂食行動を司る神経機構を解明するために、従来の動物の摂食量を指標にした解析方法では、覚醒レベルや体調などの変動要因が数多く存在する。本研究は、除脳によって精神的、心理的な食欲不振の要因を取り除き、口腔内への蒸留水注入によって誘発された嚥下様運動を指標とすることで、変動要因を極力減らし、食欲不振に関わるニューロン機構を解析できる。さらに、食欲不振と摂食行動の実行システム（嚥下）との関連も解析できる。本実験によって食欲不振だけでなく過食の神経メカニズムについても新たな発想の画期的な研究手法の開発につながる可能性があり、がん患者や高齢者の食欲不振の治療法の開発につながる可能性がある。