2020 Fiscal Year Final Research Report

Method for collecting and preserving various organs / viscera obtained from embryo-like structures derived from tissue stem cells

Research Project

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Project/Area Number	19K22946
Research Category	Grant-in-Aid for Challenging Research (Exploratory)
Allocation Type	Multi-year Fund
Review Section	Medium-sized Section 90:Biomedical engineering and related fields
Research Institution	University of Tsukuba
Principal Investigator	Oki Akinori 筑波大学, 医学医療系, 教授 (60334067)
Co-Investigator(Kenkyū-buntansha)	石川博筑波大学, 医学医療系, 研究員 (30089784) 丸島愛樹筑波大学, 医学医療系, 講師 (40722525) 松丸祐司筑波大学, 医学医療系, 教授 (70323300) 豊村順子筑波大学, 医学医療系, 研究員 (80645630) 松村明筑波大学, 医学医療系, 客員教授 (90241819) 大山晃弘筑波大学, 医学医療系, 研究員 (90538232)
Project Period (FY)	2019-06-28 – 2021-03-31
Keywords	歯髄幹細胞 / 胚様体 / 胚子様構造体 / 胚子成長因子 / 器官・臓器の原基
Outline of Final Research Achievements	For the purpose of developing a method for treating dysfunction of various organs by regenerative transplantation therapy, we focused on dental pulp stem cells, which suggest the existence of totipotential cells. Human dental pulp stem cells were cultured with hanging-drop method to prepare embryoid bodies. This embryoid body was cultured using circumfusion apparatus in a culture medium containing embryo growth factor (EmbGF) to prepare an embryoid-like structure in which heartbeat was observed. The primordia of various organs/ viscera were found in embryonic structures. The primordia of organs/viscera are as follows : heart, liver, gastrointestinal tract, neural tube, retina, kidney, gastrointestinal tract, respiratory organs, skin, skeletal muscle, arteries and veins, bone / cartilage, tooth germ, etc. A primordial blood cell population was found in the liver. The primordia of various organs/viscera were DMSO-free CryoScarless and could be stored in liquid nitrogen.
Free Research Field	産婦人科学
Academic Significance and Societal Importance of the Research Achievements	移植後の腫瘍形成がない組織幹細胞であるヒト歯髄幹細胞から、EmbGFを用いて胚子様構造体を作製し、この中に存在する各種器官・臓器の原基を採取することに成功した。これらの原基はそれらを構成する細胞をone set含有している。そこで、これら原基を機能不全に陥っている器官・臓器に移植すれば、長期にわたって高い機能の回復が期待できる。ここに本研究の学術的意義がある。また、本研究から得られたノウハウを用いて、HLAホモドナーの歯髄細胞から胚子様構造体を作製し、各種器官・臓器の原基が得られれば、他家移植可能な再生医療を提供でき、その社会的意義は計り知れない。