Development of enzyme activity-independent biosensors using microfluidic devices

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K23646
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0502:Inorganic/coordination chemistry, analytical chemistry, inorganic materials chemistry, energy-related chemistry, and related fields
• Research Institution: The University of Tokyo
• Principal Investigator: Sugimoto Yu 東京大学, 工学研究科, 特任研究員 (30848841)
• Project Period (FY): 2019-08-30 – 2021-03-31
• Keywords: バイオセンサ / メディエータ型酵素電極反応 / 微小電極 / 解析式 / 球形拡散 / 定常状態近似

Outline of Final Research Achievements

I have succeeded in deriving an analytical formula for the mediator-type enzymatic electrode reaction at the microelectrode when the enzyme activity is very high. The analytical formula reveals the condition that improves the performance of the biosensor using this system. The linear range of the current expands when the diffusion coefficient of the substrate and the Michaelis constant for the substrate are small, and the diffusion coefficient of the mediator and the microelectrode radius are large.

Free Research Field

分析化学

Academic Significance and Societal Importance of the Research Achievements

微小電極上におけるメディエータ型酵素電極反応を利用したバイオセンサは、電流値が酵素活性に依存するという、酵素電極が本来持つ弱点を克服した究極のセンサと言える。本研究では解析式導出を通じて、この反応系を完全に理解し、さらにこの系を用いたバイオセンサの性能を向上させる条件を明確に示すことに成功した。