Mobile genetic element TEM-1 beta-lactamase

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K23965
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0902:General internal medicine and related fields
• Research Institution: Kyoto University
• Principal Investigator: Noguchi Taro 京都大学, 医学研究科, 助教 (10847578)
• Project Period (FY): 2019-08-30 – 2021-03-31
• Keywords: 薬剤耐性大腸菌 / 水平伝播

Outline of Final Research Achievements

β-lactam has a critical role in treatment of bacterial infections, β-lactamase producing bacteria has become a major public health threat worldwide. To invesitigate mechanisms underlying spread of TEM-1, the most common β-lactamase in Escherichia coli, we performed genome analysis of E. coli collected from mutliple Japansese hospitals. TEM-1 in most isolates was flanked by transposon. In one isolate, TEM-1 with transposon was found in putative plasmid strucure. The structure with high similarity was also found in isolates with different genetic phylogeny, which implies horizontal transfer of TEM-1 by larger mobile genetic element than transposon. These results will contribute to control TEM-1 producing E. coli.

Free Research Field

感染症内科学

Academic Significance and Societal Importance of the Research Achievements

細菌感染症治療の中心を担うβラクタム薬に耐性を示すβラクタマーゼ産生細菌が世界的な脅威となっている。本研究は、そのβラクタマーゼの拡散要因の解明に寄与するもので、βラクタマーゼ産生菌拡散の制御に向けた研究への発展が期待できる。