The effect of periodontal disease on diabetic nephropathy

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K24062
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0907:Oral science and related fields
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Sasaki Naoki 東京医科歯科大学, 歯学部附属病院, 非常勤講師 (40848305)
• Project Period (FY): 2019-08-30 – 2021-03-31
• Keywords: 歯周病 / 糖尿病性腎症

Outline of Final Research Achievements

We investigated the effect of intravenously injected or administrated by oral gavage P.gingivalis on diabetic nephropathy. Diabetes was induced by streptozotocin injection in C57BL/6J male mice. The urine and urinary albumin 24-hours volume were increased in the P.gingivalis administrated mice. The mesangial matrix expansion and the glomerular basement membrane thickness were no difference between the mice administrated P.gingivalis and saline. The level of inflammation-related gene in renal cortex was increased in the P.gingivalis administrated mice.

Free Research Field

歯周病

Academic Significance and Societal Importance of the Research Achievements

糖尿病性腎症は、臨床的に尿中のアルブミン量の増加や腎臓の尿細管間質での慢性炎症が認められるが、本研究において、P.gingivalisを投与した糖尿病モデルマウスでこれらの臨床症状が悪化するという結果が得られた。しかし、歯周病と糖尿病性腎症の関連性についての明確なメカニズムを解明するためには、遺伝子発現の網羅的な解析および16S rRNAに基づいた腸内細菌叢解析、糸球体由来の細胞株へのP.gingivalis LPS刺激による分化への影響の評価などさらなる実験が必要である。