Effect of oral administration of phytochemicals on bone formation at tooth extraction sockets in an osteoporotic animal model

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K24135
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0907:Oral science and related fields
• Research Institution: Tohoku University
• Principal Investigator: Shishido Shunichi 東北大学, 歯学研究科, 助教 (20850613)
• Project Period (FY): 2019-08-30 – 2021-03-31
• Keywords: ファイトケミカル / ポリフェノール / プロアントシアニジン / 卵巣摘出ラット / 骨粗鬆症 / 抜歯窩

Outline of Final Research Achievements

The purpose of this study was to evaluate the effect of proanthocyanidin, a kind of phytochemicals, on alveolar bone healing of ovariectomized (OVX) rats. Female Wistar rats (7 weeks old) underwent OVX surgery, and their right-and-left upper 1st molars were extracted 1 week after the surgery. In the test group, oral administration of proanthocyanidin (100 mg/2.5 mL/kg) suspended in water was carried out once daily for 9 weeks, while water (vehicle) was administrated for rats in the control group. The maxillae excised from the animals after sacrifice were subjected to micro-computed tomography (CT) and histological analyses. The analyses revealed that there was no difference in the newly formed bone in the tooth extraction socket between test and control groups. Thus, the effect of oral administration of proanthocyanidin on the bone formation at a tooth extraction socket could not be demonstrated by the analytical methods used in the present study.

Free Research Field

歯科補綴学

Academic Significance and Societal Importance of the Research Achievements

ファイトケミカル（植物性化学物質）は安全性が高く、一般的な骨粗鬆症治療薬であるビスフォスフォネート製剤とは異なり、副作用を惹起するリスクは低い。そのため、骨粗鬆症患者の歯科治療における新たな医薬品あるいは特定保健用食品となる可能性を秘めており、本研究でその効果を示せれば社会的な意義は大きい。また、本研究ではラットの卵巣摘出術＋抜歯＋ファイトケミカル投与の実験系を用いており、今後様々なファイトケミカルの投与を通してその効果の検討や作用機序の解明を行うことができるため学術的に意義がある。