2023 Fiscal Year Final Research Report
Studies on development of control strategy for Asian zoonotic schistosomiasis with microsatellite marker system
| Project/Area Number |
19KK0173
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| Research Category |
Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 42:Veterinary medical science, animal science, and related fields
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| Research Institution | Obihiro University of Agriculture and Veterinary Medicine |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
尾針 由真 酪農学園大学, 獣医学群, 助教 (00847056)
桐木 雅史 獨協医科大学, 医学部, 講師 (50265302)
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| Project Period (FY) |
2019-10-07 – 2024-03-31
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| Keywords | 日本住血吸虫症 / 顧みられない熱帯病 / 人獣共通感染症 / スイギュウ / マイクロサテライトマーカー |
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| Outline of Final Research Achievements |
To prepare DNA from single parasite egg for multi-locus genotyping using microsatellite markers (STR/MLG), the current protocol of Miracidium Hatching Test (MHT) was modified. Two surveys were conducted in 2022 and 2023 in near the city of Tacloban, Leyte province, where the parasite eggs were collected from patients and water buffaloes. STR/MLG with the single-egg DNA samples showed gene flow among parasites distributed in these villages, suggesting zoonotic lifecycle of the parasite and human and livestock migration in the area. Serodiagnostic means needed in surveillance for schistosomiasis mekongi, which is endemic in Mekong Delta (Lao PDR and Cambodia), were examined. An ELISA using a recombinant protein (rSmekTPx-1) as antigen was validated and showed satisfied sensitivity and specificity, although cross-reactivity with some sera of opisthorchiasis patients was observed.
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| Free Research Field |
寄生虫病学
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| Academic Significance and Societal Importance of the Research Achievements |
日本住血吸虫の集団構造解析に使用する単一寄生虫卵由来のDNAサンプルを調整するため、現行のミラシジウム孵化法（MTA）に改良を加えたプロトコールを確立した。MHTの改良法を日本住血吸虫症の対策に導入することで、流行地での寄生虫の集団構造解析が可能になる。また、メコン住血吸虫症の調査研究において必要になる、血清診断法（酵素抗体法：ELISA）のプロトコールを検討した。組換体抗原を用いるELISAは、抗原の大量供給と品質管理が容易なことから、大規模調査への応用が期待できる。これらの手法の導入により、アジア型住血吸虫症の監視と排除に向けた寄生虫病対策の更なる進展が期待できる。
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