2010 Fiscal Year Final Research Report
A liposome encapsulating molecular computer : an autonomously judging drug delivery system.
| Project/Area Number |
20200007
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied pharmacology
Bioinformatics/Life informatics
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SHOHDA Koh-ichiroh The University of Tokyo, 大学院・総合文化研究科, 助教 (00401216)
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| Project Period (FY) |
2008 – 2010
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| Keywords | ドラッグデリバリー / 生命分子計算 |
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| Research Abstract |
I had developed a liposome encapsulating molecular computer : RTRACS. In the present study, a molecular umbrella (DNA-cholic acid conjugate) which permeated DNA/RNA molecule through lipid bilayer membrane was successfully synthesized. In order to check the limitation of liposome, a giant liposome was applied to PCR conditions (high temperature and hard convection). Consequently the giant liposome maintained the shape and barrier capability under the PCR conditions. The unilamellar liposome is useful for the liposome-based DDS research. Therefore we developed a novel method for giant unilamellar vesicle (GUV) preparation which based on the classical Bangham's method (natural swelling method of lipid film). Many GUVs can be prepared in the physiological condition (containing 150mM NaCl and 10mM MgCl2) without the support of oil.
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