Development of photo-driven bionano-machines

2010 Fiscal Year Final Research Report

• Project/Area Number: 20200029
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
• Allocation Type: Single-year Grants
• Research Field: Microdevices/Nanodevices; Biophysics
• Research Institution: Soka University
• Principal Investigator: MARUTA Shinsaku Soka University, 工学部, 教授 (40231732)
• Project Period (FY): 2008 – 2010
• Keywords: ナノマシーン / 生体分子機械

Research Abstract

Photochromic molecules of azobenzene and spiropyran derivatives incorporated were incorporated into the functional sites of the bionanomachines, myosin, kinesin, calmodulin, G-protein Ras. The function of the bionanomachines modified with photochromic molecules were successfully controlled by Ultra-violet (UV) and visible (VIS) light. Reversibly. Moreover, we synthesized ATP analogue composed of photochromic molecule and revealed the analogue can induce ATP state, ADP state or no nucleotide state reversibly by UV-VIS light irradiation.

Research Products

• [Journal Article] Synthesis of a novel fluorescent non-nucleotide ATP analogue and its interaction with myosin ATPase (2011)
  • Author(s): Tanaka, K., Kimura, T., Maruta, S.
  • Journal Title: Journal of Biochemistry. 149 Pages: 395-403
• [Journal Article] Crystallographic analysis reveals a unique conformation of the ADP-bound novel rice kinesin K16 (2010)
  • Author(s): Tanaka, K., Umeki, N., Mitsui,T., Fujimoto, Z., Maruta, S.
  • Journal Title: Biochem Biophys Res Commun. 401 Pages: 251-256
• [Journal Article] Kinesin-Calmodulin fusion protein as a molecular shuttle (2010)
  • Author(s): Shishido, H., Nakazato, K., Katayama, E., Chaen, S., Maruta, S.
  • Journal Title: Journal of Biochemistry 147 Pages: 213-223
• [Journal Article] Photocontrol of calmodulin interaction with target peptides using azobenzene derivative (2009)
  • Author(s): Shishido, H., Yamada, M.D., Kondo K., Maruta, S.
  • Journal Title: Journal of Biochemistry. 146 Pages: 581-590
• [Presentation] Photo-regulation of kinesin ATPase using microtubules modified with photochromic molecule (2011)
  • Author(s): Ishiyama, K., Itaba, T., Shishido, H., Maruta, S.
  • Organizer: 第55回米国生物物理学会
  • Place of Presentation: Baltimore, MD USA.
  • Year and Date: 2011-03-08
• [Presentation] Synthesis of photochromic ATP analogue and its interaction with motor protein (2011)
  • Author(s): Aritomi, K., Kimura, T., Itaba, T., Maruta, S.
  • Organizer: 第55回米国生物物理学会
  • Place of Presentation: Baltimore, MD USA.
  • Year and Date: 2011-03-08
• [Presentation] Phtocontrol of the small G protein Ras using photochromic molecule (2011)
  • Author(s): Kobayashi, H., Maruta, S.
  • Organizer: 第55回米国生物物理学会
  • Place of Presentation: Baltimore, MD USA.
  • Year and Date: 2011-03-08
• [Presentation] Conformational change of myosin head induced by phto-isomerization of photochromic ATP analogue (2011)
  • Author(s): Itaba, T., Shishido, H., Sugimoto, Y., Wakabayashi, K., Maruta, S.
  • Organizer: 第55回米国生物物理学会
  • Place of Presentation: Baltimore, MD USA.
  • Year and Date: 2011-03-08
• [Presentation] Phtocontrol of myosin V ATPase activity using calmodulin modified with photochromic compound (2011)
  • Author(s): Shishido, H., Ikebe, M., Maruta, S.
  • Organizer: 第55回米国生物物理学会
  • Place of Presentation: Baltimore, MD USA.
  • Year and Date: 2011-03-08
• [Presentation] Funcrion control of kinesin using functional loop L5 of kinesin modified with photochromic molecule (2011)
  • Author(s): Ishikawa, K., Tanaka, K., Maruta, S.
  • Organizer: 第55回米国生物物理学会
  • Place of Presentation: Baltimore, MD USA.
  • Year and Date: 2011-03-06
• [Presentation] Ca2+ dependent dimerization of kinesin-CaM and Kinesin-M13 fusion proteins (2011)
  • Author(s): Nakazato, K., Shishido, H., Itaba, T., Kondo, K., Maruta, S.
  • Organizer: 第55回米国生物物理学会
  • Place of Presentation: Baltimore, MD USA.
  • Year and Date: 2011-03-06
• [Presentation] 光制御型生体分子機械の開発 (2011)
  • Author(s): 宍戸英樹、小林寿、丸田晋策
  • Organizer: 生体運動研究合同班会議
  • Place of Presentation: 大阪
  • Year and Date: 2011-01-07
• [Presentation] Calcium dependent dimerization of kinesin chimera proteins fused with calmodulin and M13 peptide (2010)
  • Author(s): Takeshi Itaba, Kiyoshi Nakazato, Hideki Shishido, Shinsaku Maruta
  • Organizer: 第83回日本生化学会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-09
• [Presentation] Interaction of n ovel fluorescent non-nucleotide ATP analogue with myosin S1 (2010)
  • Author(s): Keiko Tanaka, Taro Kimura, Shinsaku Maruta
  • Organizer: 第83回日本生化学会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-07
• [Presentation] Conformational change of α1 helix of kinesin (2010)
  • Author(s): Satoshi Yasuda, Masafumi D.Yamada, Shinsaku Maruta, Toshiaki Arata
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 仙台
  • Year and Date: 2010-09-21
• [Presentation] Control of kinesin ATPase activity by modification of functional loop L5 (2010)
  • Author(s): Kumiko Ishikawa, Keiko Tanaka, Shinsaku Maruta
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 仙台
  • Year and Date: 2010-09-21
• [Presentation] Photo-regulation of kinesin ATPase using microtubules modified with photochromic molecules (2010)
  • Author(s): Koichiro Ishiyama, Takeshi Itaba, Hideki Shishido, Shinsaku Maruta
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 仙台
  • Year and Date: 2010-09-21
• [Presentation] Photocontrol of the small G-protein Ras using photochromic molecule (2010)
  • Author(s): Hisashi Kobayashi, Hideki Shishido, Shinsaku Maruta
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 仙台
  • Year and Date: 2010-09-21
• [Presentation] Interaction of photochromic ATP analogue with skeletal and smooth muscle myosin (2010)
  • Author(s): Takeshi Itaba, Hideki Shishido, Yasunobu Sugimoto, Katsuzo Wakabayashi, Shinsaku Maruta
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 仙台
  • Year and Date: 2010-09-21
• [Presentation] Synthesis of photochromic ATP analogue and its interaction with kinesin (2010)
  • Author(s): Kazuya Aritomi, Taro Kimura, Takeshi Itaba, Shinsaku Maruta
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 仙台
  • Year and Date: 2010-09-21
• [Presentation] Interaction of novel fluorescent non-nucleotide ATP analogue with ATP-driven molecular motors (2010)
  • Author(s): Keiko Tanaka, Taro Kimura, Shinsaku Maruta
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 仙台
  • Year and Date: 2010-09-20
• [Presentation] Photocontrol of myosin V ATPase using calmodulin modified with azobenzene derivative (2010)
  • Author(s): Hideki Shishido, Shinsaku Maruta
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 仙台
  • Year and Date: 2010-09-20
• [Presentation] Ca2+ dependent dimerization of kinesin-CaM and kinesin-M13 fusion proteins (2010)
  • Author(s): Kiyoshi Nakazato, Hideki Shishido, Takeshi Itaba, Kazunori Kondo, Shinsaku Maruta
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 仙台
  • Year and Date: 2010-09-20
• [Presentation] KINESIN-CALMODULIN FUSION PROTEIN AS A MOLECULAR SHUTTLE AND MARKER FOR PLUS END OF MICROTUBULE (2010)
  • Author(s): Itaba, T., Shishido, H., Nakazato, K., Katayama, E., Chaen, S., Maruta, S.
  • Organizer: 第54回米国生物物理学会
  • Place of Presentation: San Francisco, CA USA.
  • Year and Date: 2010-02-22
• [Presentation] ANALYSIS OF THE ROLE OF UNIQUE LOOP L5 IN RICE KINESIN K16 MOTOR DOMAIN (2010)
  • Author(s): Ishikawa, K., Tanaka, K., Maruta, S.
  • Organizer: 第54回米国生物物理学会
  • Place of Presentation: San Francisco, CA USA.
  • Year and Date: 2010-02-21
• [Presentation] ANALYSIS OF CONFORMATIONAL CHANGE OF CONVENTIONAL KINESIN CHIMERIC PROTEIN FUSED WITH GFP USING SMALL ANGLE X-RAY SOLUTION SCATTERING (2010)
  • Author(s): Tanaka, K., Sugimoto, Y., Wakabayashi, K., Maruta, S.
  • Organizer: 第54回米国生物物理学会
  • Place of Presentation: San Francisco, CA USA.
  • Year and Date: 2010-02-21
• [Presentation] SYNTHESIS OF PHOTOCHROMIC ATP ANALOGUE AND ITS INTERACTION WITH MOTOR PROTEINS (2010)
  • Author(s): Aritomi, K., Kimura, T., Maruta, S.
  • Organizer: 第54回米国生物物理学会
  • Place of Presentation: San Francisco, CA USA.
  • Year and Date: 2010-02-21
• [Presentation] SYNTHESIS OF NOVEL FLUORESCENT ATP ANALOGUE AND ITS INTERACTION WITH NUCLEOTIDE DEPENDENT MOTOR PROTEINS (2010)
  • Author(s): Kimura, T., Yamada, M.D., Ito, M., Maruta, S.
  • Organizer: 第54回米国生物物理学会
  • Place of Presentation: San Francisco, CA USA.
  • Year and Date: 2010-02-21
• [Presentation] キネシン-カルモジュリン融合蛋白質を用いた分子シャトルの開発 (2010)
  • Author(s): 宍戸英樹、中里清史、片山栄作、茶圓茂、丸田晋策
  • Organizer: 生体運動研究合同班会議
  • Place of Presentation: 東京
  • Year and Date: 2010-01-10
• [Presentation] Synthesis of novel fluorescent ATP analogue and interaction with nucleotide dependent motor protein. (2009)
  • Author(s): Taro KIMURA, Masafumi YAMADA, Masato ITO, Shinsaku MARUTA
  • Organizer: Biophysical Society 53^<rd> Annual Meeting Biophysical Journal Abstracts Issue : p.133a
  • Place of Presentation: Boston USA
  • Year and Date: 20090200
• [Presentation] Photo-sctivation of ATPase activity of Caged-kinesin. (2009)
  • Author(s): Yuki TAKANO, Kazuya ARITOMI, Masafumi D. YAMADA, Shinsaku MARUTA
  • Organizer: Biophysical Society 53^<rd> Annual Meeting Biophysical Journal Abstracts Issue : p.134a
  • Place of Presentation: Boston USA
  • Year and Date: 20090200
• [Presentation] Photo-regulation of kinesin ATPase activity using photochromic molecule. (2009)
  • Author(s): Takeshi ITABA, Masafumi D. YAMADA, Shinsaku MARUTA
  • Organizer: Biophysical Society 53^<rd> Annual Meeting Biophysical Journal Abstracts Issue : p.134a
  • Place of Presentation: Boston USA
  • Year and Date: 20090200
• [Presentation] Photo-control of ATPase activity of the kinesin motor domain intermolecularly cross-linked by bifunctional photochromic compound. (2009)
  • Author(s): Eiichi KOBAYASHI, Masafumi YAMADA, Masato ITO, Shinsaku MARUTA
  • Organizer: Biophysical Society 53^<rd> Annual Meeting Biophysical Journal Abstracts Issue : p.134a
  • Place of Presentation: Boston USA
  • Year and Date: 20090200
• [Presentation] Photo-control of myosin Va using photoresponsive calmodulin. (2009)
  • Author(s): Hideki SHISHIDO, Nobuhisa UMEKI, Osamu SATO, Mitsuo IKEBE, Shinsaku MARUTA
  • Organizer: Biophysical Society 53^<rd> Annual Meeting Biophysical Journal Abstracts Issue : p.138a
  • Place of Presentation: Boston USA
  • Year and Date: 20090200
• [Presentation] 光制御型カルモジュリンの生体分子モーターへの応用 (2009)
  • Author(s): 宍戸英樹、山田正文、丸田晋策
  • Organizer: 生体運動研究合同班会議
  • Place of Presentation: 東京
  • Year and Date: 20090100
• [Presentation] Kinesin-Calmodulin Fusion Protein as a Molecular Shuttle (2009)
  • Author(s): Shishidol, H., Nakazato, K., Katayama, E., Chaen, S., Maruta, S.
  • Organizer: 第49回米国細胞生物学会年会
  • Place of Presentation: San Diego, CA USA.
  • Year and Date: 2009-12-07
• [Presentation] Synthesis of Novel Fluorescent GTP Analogue and Its Interaction with Small G-Protein Ras (2009)
  • Author(s): Kobayashi, H., Kimura, T., Yamada, M.D., Maruta, S.
  • Organizer: 第49回米国細胞生物学会年会
  • Place of Presentation: San Diego, CA USA.
  • Year and Date: 2009-12-07
• [Presentation] Photo-regulation of kinesin function using azobenzene derivative (2009)
  • Author(s): Takeshi Itaba, Masafumi Yamada, Shinsaku Maruta
  • Organizer: 第47回日本生物物理学会年会
  • Place of Presentation: 徳島
  • Year and Date: 2009-10-31
• [Presentation] The functional role of the unique loop L5 of kinesin (2009)
  • Author(s): Kumiko Ishikawa, Keiko Tanaka, Shinsaku Maruta
  • Organizer: 第47回日本生物物理学会年会
  • Place of Presentation: 徳島
  • Year and Date: 2009-10-31
• [Presentation] Synthesis of novel fluorescent ATP analogue and interaction with nucleotide dependent motor proteins (2009)
  • Author(s): Taro Kimura, Masafumi D, Yamada, Shinsaku Maruta
  • Organizer: 第47回日本生物物理学会年会
  • Place of Presentation: 徳島
  • Year and Date: 2009-10-31
• [Presentation] Analysis of conformational change of kinesin neck-linker using using small angle X-ray solution scattering (2009)
  • Author(s): Keiko Tanaka, Yasunobu Sugimoto, Masafumi D.Yamada, Katsuzo Wakabayashi, Shinsaku Maruta
  • Organizer: 第47回日本生物物理学会年会
  • Place of Presentation: 徳島
  • Year and Date: 2009-10-31
• [Presentation] Kinesin Calmodulin Fusion Protein as a Molecular Shuttle (2009)
  • Author(s): Hideki Shishido, Kiyoshi Nakazato, Eisaku Katayama, Shigeru Chaen, Shinsaku Maruta
  • Organizer: 第47回日本生物物理学会年会
  • Place of Presentation: 徳島
  • Year and Date: 2009-10-30
• [Presentation] Application of Kinesin Calmodulin Fusion Protein as An Molecular Shuttle (2009)
  • Author(s): Hideki Shishido, Kiyoshi Nakazato, Eisaku Katayama, Shigeru Chaen, Shinsaku Maruta
  • Organizer: 第82回日本生化学会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-24
• [Presentation] Photo-regulation of kinesin using photo-responsive caged compound (2009)
  • Author(s): Kazuya Aritomi, Masafumi Yamada, Shinsaku Maruta
  • Organizer: 第82回日本生化学会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-24
• [Presentation] Analysis of crystal structure of the motor domain of rice kinesin K16 and comparison with the structures of other related kinesin. (2008)
  • Author(s): Keiko TANAKA, Toshiaki MITSUI, Zui FUJIMOTO, Shinsaku MARUTA
  • Organizer: 第46回日本生物物理学会 同学会要旨集p.S102
  • Place of Presentation: 福岡
  • Year and Date: 20081200
• [Presentation] Photo-control of ATPase activity of the kinesin motor domains intermolecularly cross-linked by bifunctional photochromic compound. (2008)
  • Author(s): Eiichi KOBAYASHI, Masafumi YAMADA, Masato ITO, Shinsaku MARUTA
  • Organizer: 第46回日本生物物理学会 同学会要旨集p.S102
  • Place of Presentation: 福岡
  • Year and Date: 20081200
• [Presentation] Photo-control of calmodulin target protein using photochromic compound. (2008)
  • Author(s): Hideki SHISHIDO, Kiyoshi NAKAZATO, Mitsuo IKEBE, Shinsaku MARUTA
  • Organizer: 第46回日本生物物理学会 同学会要旨集p.S150
  • Place of Presentation: 福岡
  • Year and Date: 20081200
• [Presentation] Photo-regulation of kinesin using photo-responsible caged compound. (2008)
  • Author(s): Kazuya ARITOMI, Masafumi D. YAMADA, Shinsaku MARUTA
  • Organizer: 第46回日本生物物理学会 同学会要旨集p.S151
  • Place of Presentation: 福岡
  • Year and Date: 20081200
• [Presentation] Photo-regulation of kinesin ATPase activity using photochromic molecule. (2008)
  • Author(s): Takeshi ITABA, Masafumi D. YAMADA, Shinsaku MARUTA
  • Organizer: 第46回日本生物物理学会 同学会要旨集p.S152
  • Place of Presentation: 福岡
  • Year and Date: 20081200
• [Presentation] Photocontrol of calmodulin function using photochromic compound. (2008)
  • Author(s): Hideki SHISHIDO, Masafumi D. YAMADA, Kazunori KONDO, Shinsaku MARUTA
  • Organizer: 2^<nd> International symposium on Bio-nanosystems Abstract : p.34
  • Place of Presentation: Tokyo
  • Year and Date: 20081100
• [Presentation] Photoregulation of kinesin activity using an caged compounds. (2008)
  • Author(s): Masafumi D. YAMADA, E-sin CHIA, Hidekatsu MAEDA, Shinsaku MARUTA
  • Organizer: 2^<nd> International symposium on Bio-nanosystems Abstract : p.68
  • Place of Presentation: Tokyo
  • Year and Date: 20081100
• [Presentation] Photo-control of ATPase activity of the kinesin motor domains intermolecularly cross-linked by bifunctional photochromic compound. (2008)
  • Author(s): Eiichi KOBAYASHI, Masafumi YAMADA, Masato ITO, Shinsaku MARUTA
  • Organizer: 2^<nd> International symposium on Bio-nanosystems Abstract : p.69
  • Place of Presentation: Tokyo
  • Year and Date: 20081100
• [Remarks] ホームページ
  • URL: http://www.t.soka.ac.jp/maruta-lab/