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2011 Fiscal Year Final Research Report

Innovative High-speed AFM for Elucidating Vital Phenomena

Research Project

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Project/Area Number 20221006
Research Category

Grant-in-Aid for Scientific Research (S)

Allocation TypeSingle-year Grants
Research Field Nanomaterials/Nanobioscience
Research InstitutionKanazawa University

Principal Investigator

ANDO Toshio  金沢大学, 数物科学系, 教授 (50184320)

Co-Investigator(Renkei-kenkyūsha) UCHIHASHI Takayuki  金沢大学, 数物科学系, 准教授 (30326300)
WATANABE Takuo  金沢大学, 医学系研究科, 准教授 (40303268)
Project Period (FY) 2008-05-12 – 2013-03-31
Keywords1分子イメージング / 高速AFM / ダイナミクス / タンパク質 / 細胞
Research Abstract

I have accomplished, ahead of the world, the development of a high-speed atomic force microscope that can directly visualize dynamic phenomena occurring in molecules and cells at high resolution. Consequently, dynamic structural changes and molecular processes of proteins in action as well as dynamic phenomena occurring in live cells have been successfully filmed. Thus, the usefulness and innovative power of this new microscope have been demonstrated.

  • Research Products

    (46 results)

All 2013 2012 2011 2010 2009 2008 Other

All Journal Article (23 results) (of which Peer Reviewed: 23 results) Presentation (13 results) Book (4 results) Remarks (3 results) Patent(Industrial Property Rights) (3 results)

  • [Journal Article] Role of trimer-trimer interaction of bacteriorhodopsin studied by optical spectroscopy and high-speed atomic force microscopy2013

    • Author(s)
      H. Yamashita, K. Inoue, M. Shibata, T. Uchihashi, J. Sasaki, H. Kandori, and T. Ando
    • Journal Title

      J. Struct. Biol.

      Volume: (accepted)

    • DOI

      DOI:10.1016/j.jsb.2013.02.011

    • Peer Reviewed
  • [Journal Article] High-speed AFM and applications to biomolecular systems2013

    • Author(s)
      T. Ando, T. Uchihashi, and N. Kodera
    • Journal Title

      Annu. Rev. Biophys

      Volume: 42 Pages: 393-414

    • DOI

      DOI:10.1146/annurev-biophys-083012-130324

    • Peer Reviewed
  • [Journal Article] High-speed atomic force microscopy2013

    • Author(s)
      T. Ando
    • Journal Title

      Microscopy

      Volume: 62 Pages: 81-93

    • DOI

      DOI:10.1093/jmicro/dfs093

    • Peer Reviewed
  • [Journal Article] Molecular Machines directly observed by high-speed atomic force microscopy2012

    • Author(s)
      T. Ando
    • Journal Title

      FEBS Lett

      Volume: 587 Pages: 997-1007

    • DOI

      DOI:10.1016/j.febslet.2012.12.024

    • Peer Reviewed
  • [Journal Article] High-speed atomic force microscopy2012

    • Author(s)
      T. Ando, T. Uchihashi, and N. Kodera
    • Journal Title

      Jpn. J. Appl. Phys

      Volume: 51 Pages: 08KA02

    • DOI

      DOI:10.1143/JJAP.51.08KA02

    • Peer Reviewed
  • [Journal Article] Single molecule imaging on living bacterial cell surface by high-speed AFM2012

    • Author(s)
      H. Yamashita, A. Taoka, T. Uchihashi, T. Asano, T. Ando, and Y. Fukumori
    • Journal Title

      J. Mol. Biol.

      Volume: 422 Pages: 300-309

    • DOI

      DOI:10.1016/j.jmb.2012.05.018

    • Peer Reviewed
  • [Journal Article] High-speed atomic force microscopy coming of age2012

    • Author(s)
      T. Ando
    • Journal Title

      Nanotechnology

      Volume: 23 Pages: 062001

    • DOI

      DOI:10.1088/0957-4484/23/6/062001

    • Peer Reviewed
  • [Journal Article] Guide to video recording of structure dynamics and dynamic processes of proteins by high-speed atomic force microscopy2012

    • Author(s)
      T. Uchihashi, N. Kodera, and T. Ando
    • Journal Title

      Nature Protocols

      Volume: 7 Pages: 1193-1206

    • DOI

      DOI:10.1038/nprot.2012.047

    • Peer Reviewed
  • [Journal Article] Traffic jams reduce hydrolytic efficiency of cellulase on cellulose surface2011

    • Author(s)
      K. Igarashi, T. Uchihashi, A. Koivula, M. Wada, S. Kimura, T. Okamoto, M. Penttila, T. Ando, and M. Samejima
    • Journal Title

      Science

      Volume: 333 Pages: 1279-1282

    • DOI

      DOI:10.1126/science.1208386

    • Peer Reviewed
  • [Journal Article] High-speed atomic force microscopy reveals rotary catalysis of rotorless F1-ATPase2011

    • Author(s)
      T. Uchihashi, R. Iino, T. Ando, and H. Noji
    • Journal Title

      Science

      Volume: 333 Pages: 755-758

    • DOI

      DOI:10.1126/science.1205510

    • Peer Reviewed
  • [Journal Article] Structural changes in bacteriorhodopsin in response to alternate illumination observed by high-speed atomic force microscopy2011

    • Author(s)
      M. Shibata, T. Uchihashi, H. Yamashita, H. Kandori, and T. Ando
    • Journal Title

      Angew. Chem. Int. Ed.

      Volume: 50 Pages: 4410-4413

    • DOI

      DOI:10.1002/anie.201007544

    • Peer Reviewed
  • [Journal Article] Video imaging of walking myosin V by high-speed atomic force microscopy.2010

    • Author(s)
      N. Kodera, D. Yamamoto, R. Ishikawa, and T. Ando
    • Journal Title

      Nature

      Volume: Nature Pages: 72-76

    • DOI

      DOI:10.1038/nature09450

    • Peer Reviewed
  • [Journal Article] Deciphering the structure, growth and assembly of amyloid-like fibrils using high-speed atomic force microscopy2010

    • Author(s)
      P.-E. Milhiet, D. Yamamoto, O. Berthoumieu, P. Dosset, Ch. Le Grimellec, J.-M. Verdier, S. Marchal, and T. Ando
    • Journal Title

      PLoS One

      Volume: 5 Pages: e13240

    • DOI

      DOI:10.1371/journal.pone.0013240

    • Peer Reviewed
  • [Journal Article] High-speed atomic force microscopy techniques for observing dynamic biomolecular processes2010

    • Author(s)
      D. Yamamoto, T. Uchihashi, N. Kodera, H. Yamashita, S. Nishikori, T. Ogura, M. Shibata, and T. Ando
    • Journal Title

      Methods Enzymol

      Volume: 475 Pages: 541-564

    • DOI

      DOI:10.1016/S0076-6879(10)75020-5

    • Peer Reviewed
  • [Journal Article] Visualization and structural analysis of the bacterial magnetic organelle magnetosome using atomic force microscopy2010

    • Author(s)
      D. Yamamoto, A. Taoka, T. Uchihashi, H. Sasaki, H. Watanabe, T. Ando, and Y. Fukumori
    • Journal Title

      Proc. Natl. Acad. Sci. USA

      Volume: 107 Pages: 9382-9387

    • DOI

      DOI:10.1073/pnas.1001870107

    • Peer Reviewed
  • [Journal Article] High-speed atomic force microscopy shows dynamic molecular processes in photo-activated bacteriorhodopsin, Nature Nanotechnol.2010

    • Author(s)
      M. Shibata, H. Yamashita, T. Uchihashi, H. Kandori, and T. Ando
    • Journal Title

      Nature Nanotechnol

      Volume: 5 Pages: 208-212

    • DOI

      DOI:10.1038/NNANO.2010.7

    • Peer Reviewed
  • [Journal Article] Streptavidin 2D crystal substrates for visualizing biomolecular processes by atomic force microscopy2009

    • Author(s)
      D. Yamamoto, N. Nagura, S. Omote, M. Taniguchi, and T. Ando
    • Journal Title

      Biophys. J

      Volume: 97 Pages: 2358-2367

    • DOI

      DOI:10.1016/j.bpj.2009.07.046

    • Peer Reviewed
  • [Journal Article] Dynamics of bacteriorhodopsin 2D crystal observed by high-speed atomic force microscopy2009

    • Author(s)
      H. Yamashita, K. Voitchovsky, T. Uchihashi, S. Antoranz Contera, J. F. Ryan, and T. Ando
    • Journal Title

      J. Struct. Biol

      Volume: 167 Pages: 153-158

    • DOI

      DOI:10.1016/j.jsb.2009.04.011

    • Peer Reviewed
  • [Journal Article] High-speed atomic force microscopy for nano-visualization of dynamic biomolecular processes2008

    • Author(s)
      T. Ando, T. Uchihashi, and T. Fukuma
    • Journal Title

      Progr. Surf. Sci

      Volume: 83 Pages: 337-437

    • DOI

      DOI:10.1016/j.progsurf.2008.09.001

    • Peer Reviewed
  • [Journal Article] Anisotropic diffusion of point defects in two-dimensional crystal of streptavidin observed by high-speed atomic force microscopy2008

    • Author(s)
      D. Yamamoto, T. Uchihashi, N. Kodera, and T. Ando
    • Journal Title

      Nanotechnology

      Volume: 19 Pages: 384009

    • DOI

      DOI:10.1088/0957-4484/19/38/384009

    • Peer Reviewed
  • [Journal Article] Visualization of intrinsically disordered regions of proteins by high-speed atomic force microscopy2008

    • Author(s)
      A. Miyagi, Y. Tsunaka, T. Uchihashi, K. Mayanagi, S. Hirose, K. Morikawa, and T. Ando
    • Journal Title

      Chem. Phys. Chem

      Volume: 9 Pages: 1859-1866

    • DOI

      DOI:10.1002/cphc.200800210

    • Peer Reviewed
  • [Journal Article] High resonance frequency force microscope scanner using inertia balance support2008

    • Author(s)
      T. Fukuma, Y. Okazaki, N. Kodera, T. Uchihashi, and T. Ando
    • Journal Title

      Appl. Phys. Lett

      Volume: 92 Pages: 243119

    • DOI

      DOI:10.1063/1.2951594

    • Peer Reviewed
  • [Journal Article] High-speed AFM and nano-visualization of biomolecular processes2008

    • Author(s)
      T. Ando, T. Uchihashi, N. Kodera, D. Yamamoto, M. Taniguchi, A. Miyagi, and H. Yamashita
    • Journal Title

      Pflugers Archiv -Eur. J. Physiol

      Volume: 56 Pages: 211-225

    • DOI

      DOI:10.1007/s00424-007-0406-0

    • Peer Reviewed
  • [Presentation] High-speed Atomic Force Microscopy coming of age2011

    • Author(s)
      T. Ando, N. Kodera, T. Uchihashi
    • Organizer
      19th International Colloquium of Scanning Probe Microscopy
    • Place of Presentation
      Lake Toya, Hokkaido, Japan
    • Year and Date
      20111219-21
  • [Presentation] High-speed atomic force microscopy for filming biomolecular processes2011

    • Author(s)
      T. Ando
    • Organizer
      AVS 58th Annual International Symposium, Applied Surface Science Div., Advances in Scanning Probe Microscopy
    • Place of Presentation
      Nashville, TN, USA
    • Year and Date
      20111030-1104
  • [Presentation] High-speed bio-AFM coming of age2011

    • Author(s)
      T. Ando
    • Organizer
      4th AFM BioMed Conference
    • Place of Presentation
      Institut Curie, Paris
    • Year and Date
      20110823-27
  • [Presentation] Direct observation of molecular machines by high-speed atomic force microscopy2011

    • Author(s)
      T. Ando
    • Organizer
      25th Anniversary Symposium of Protein Society, Molecular machines
    • Place of Presentation
      Boston, MA, USA
    • Year and Date
      20110723-27
  • [Presentation] Motor proteins in action filmed by high-speed AFM2011

    • Author(s)
      T. Ando
    • Organizer
      Gordon Research Conference on Muscle and Molecular Motors
    • Place of Presentation
      New London, NH, USA
    • Year and Date
      20110710-15
  • [Presentation] Direct and dynamic visualization of protein molecules in action by high-speed AFM2011

    • Author(s)
      T. Ando
    • Organizer
      European Science Foundation Research Conference on Biological Surfaces and Interfaces
    • Place of Presentation
      Sant Feliu de Guixols, Spain
    • Year and Date
      20110626-0701
  • [Presentation] Dynamic imaging of protein molecules in action by high-speed atomic force microscopy2011

    • Author(s)
      T. Ando
    • Organizer
      5th IUMAS & ALC'11 Conference
    • Place of Presentation
      Olympic Parktel, Seoul,Korea
    • Year and Date
      20110523-27
  • [Presentation] Dynamic processes of proteins filmed by high-speed AFM2011

    • Author(s)
      T. Ando, T. Uchihashi, and N. Kodeara
    • Organizer
      XIII Linz Winter Workshop
    • Place of Presentation
      Linz, Austria
    • Year and Date
      20110204-07
  • [Presentation] Video imaging of biomolecular processes by high-speed AFM2011

    • Author(s)
      T. Ando
    • Organizer
      IEEE MEMS2011 Conference: The 24th International Conference on Micro Electro Mechanical Systems
    • Place of Presentation
      Cancun, Mexico
    • Year and Date
      20110123-27
  • [Presentation] Direct visualization of walking myosin V molecules by high-speed atomic forcemicroscopy2011

    • Author(s)
      T. Ando
    • Organizer
      Motility Subgroup Symposium at Biophysical Society 55th Annual Meeting
    • Place of Presentation
      Baltimore, Maryland
    • Year and Date
      2011-03-05
  • [Presentation] Dynamic imaging of biomolecular processes by high-speed AFM2010

    • Author(s)
      T. Ando
    • Organizer
      Recent Advances and Future Prospects for Visualizing Macromolecular Complexes and CellularStructures
    • Place of Presentation
      NIH, Bethesda, USA
    • Year and Date
      20101012-13
  • [Presentation] High-speed AFM imaging of intrinsically disordered proteins2010

    • Author(s)
      T. Ando
    • Organizer
      IRB Barcelona BioMed Conference on "Intrinsically Disordered Proteins in Biomedicine"
    • Place of Presentation
      Barcelona, Spain
    • Year and Date
      20101004-06
  • [Presentation] Dynamic visualization of protein molecules in action by high-speed AFM2010

    • Author(s)
      T. Ando
    • Organizer
      UK-SPM 2010
    • Place of Presentation
      ExCel, London
    • Year and Date
      20100630-0701
  • [Book] Biophysics for the Life Sciences Vol 22013

    • Author(s)
      T. Uchihashi, N. Kodera, T. Ando
    • Total Pages
      119-147
    • Publisher
      Springer
  • [Book] Springer series Methods in Molecular Biology2012

    • Author(s)
      T. Ando, N. Kodera
    • Total Pages
      57-69
    • Publisher
      Springer
  • [Book] Lecture Notes inControl and Information Sceinces, Vol.4132011

    • Author(s)
      T. Ando
    • Publisher
      Springer
  • [Book] Atomic force microscopy in liquid2011

    • Author(s)
      T. Ando, T. Uchihashi, N. Kodera, M. Shibata, D. Yamamoto, H. Yamashita
    • Total Pages
      189-210
    • Publisher
      Wiley-VCH Verlag GmbH
  • [Remarks]

    • URL

      http://www.s.kanazawa-u.ac.jp/phys/biophys/index.htm (研究室)

  • [Remarks]

    • URL

      http://www.se.kanazawa-u.ac.jp/bioafm_center/j/index.htm

  • [Remarks]

    • URL

      http://www.se.kanazawa-u.ac.jp/bioafm_center/index.htm

  • [Patent(Industrial Property Rights)] 原子間力顕微鏡及びそのカンチレバー支持具2010

    • Inventor(s)
      安藤敏夫,岡崎康孝,内橋貴之
    • Industrial Property Rights Holder
      金沢大学
    • Industrial Property Number
      特願2010-126027
    • Filing Date
      2010-06-01
  • [Patent(Industrial Property Rights)] 走査プローブ顕微鏡のスキャナー装置2008

    • Inventor(s)
      福間剛士,安藤敏夫,岡崎康孝
    • Industrial Property Rights Holder
      金沢大学
    • Industrial Property Number
      特願2008-147041
    • Filing Date
      2008-06-04
  • [Patent(Industrial Property Rights)] 走査プローブ顕微鏡2008

    • Inventor(s)
      安藤敏夫,内橋貴之,古寺哲幸,他
    • Industrial Property Rights Holder
      金沢大学
    • Industrial Property Number
      特願2008-275981
    • Filing Date
      2008-10-27

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Published: 2014-08-29  

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