2012 Fiscal Year Final Research Report
Dynamic Proteomics of Transcriptional and Nuclear Architecture
| Project/Area Number |
20221010
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| Research Category |
Grant-in-Aid for Scientific Research (S)
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| Allocation Type | Single-year Grants |
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| Research Field |
Living organism molecular science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
HAMAKUBO Takao 東京大学, 先端科学技術研究センター, 教授 (90198797)
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| Co-Investigator(Kenkyū-buntansha) |
NAITO Makoto 新潟大学, 医歯学系, 教授 (30045786)
MIYOSHI Motosuke 東京大学, 先端科学技術研究センター, 特任教授 (40345137)
IHARA Shigeo 東京大学, 先端科学技術研究センター, 特任教授 (30345136)
MOCHIZUKI Yasuhiro 東京大学, 先端科学技術研究センター, 特任准教授 (80282523)
IWANARI Hiroko 東京大学, 先端科学技術研究センター, 特任助教 (20176556)
KAWAMURA Takeshi 東京大学, 先端科学技術研究センター, 特任助教 (70306835)
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| Co-Investigator(Renkei-kenkyūsha) |
SAKIHAMA Toshiko 東京大学, 先端科学技術研究センター, 特任准教授 (70187061)
DAIGO Kenji 東京大学, 先端科学技術研究センター, 特任助教 (20466866)
HORIUCHI Keiko 東京大学, 先端科学技術研究センター, 特任助教 (00456203)
OHTA Yoshihiro 東京大学, 先端科学技術研究センター, 特任助教 (80436592)
OHASHI Riuko 新潟大学, 医歯学系, 助教 (20447600)
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| Project Period (FY) |
2008 – 2012
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| Keywords | プロテオミクス / 複合体解析 / スプライシング / 転写調節 / 細胞周期 / 核スペックル / 核小体 / クロマチン修飾 |
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| Research Abstract |
In this study, we developed a highly sensitive quantitative proteomics analysis technique by using magnetic beads with immobilized specific antibodies. By generating highly specific monoclonal antibodies against HNF4-alpha, LXR-alpha, or WTAP, we have identified protein complexes for the transcriptional control of glucose metabolism related genes or RNA-processing machinery for the cell cycle control through distribution to nuclear speckles or nucleoli.
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[Journal Article] G-protein-coupled receptor inactivation by an allosteric inverse-agonist antibody2012
Author(s)
Hino T, Arakawa T, Iwanari H, Yurugi-Kobayashi T, Ikeda-Suno C, Nakada-Nakura Y, Kusano-Arai O, Weyand S, Shimamura T, Nomura N, Cameron AD, Kobayashi T, Hamakubo T,Iwata S, Murata T
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Journal Title
Nature
Volume: 482(7384)
Pages: 237-240
DOI
Peer Reviewed
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[Journal Article] The proteomic profile of circulating pentraxin 3 (PTX3) complex in sepsis demonstrates the interaction with azurocidin 1 and other components of neutrophil extracellular traps2012
Author(s)
Daigo K, Yamaguchi N, Kawamura T, Matsubara K, Jiang S, Ohashi R, Sudou Y, Kodama T, Naito M, Inoue K, Hamakubo T
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Journal Title
Mol Cell Proteomics
Volume: 11(6)
Pages: M111.015073
DOI
Peer Reviewed
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[Journal Article] Epigenetically coordinated GATA2 binding is necessary for endothelium-specific endomucinexpression2011
Author(s)
Kanki Y, Kohro T, Jiang S, Tsutsumi S, Mimura I, Suehiro J, Wada Y, Ohta Y, Ihara S, Iwanari H, NaitoM, Hamakubo T, Aburatani H, Kodama T, Minami T
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Journal Title
EMBO J
Volume: 30(13)
Pages: 2582-2595
DOI
Peer Reviewed
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[Journal Article] Proteomic analysis of native hepatocyte nuclear factor-4α (HNF4α) isoforms, phosphorylation status, and interactive cofactors2011
Author(s)
Daigo K, Kawamura T, Ohta Y, Ohashi R, Katayose S, Tanaka T, Aburatani H, Naito M, Kodama T, Ihara S, Hamakubo T
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Journal Title
J Biol Chem
Volume: 286(1)
Pages: M110.154732
DOI
Peer Reviewed
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[Journal Article] A wave of nascent transcription on activated human genes2009
Author(s)
Wada Y, Ohta Y, Xu M, Tsutsumi S, Minami T, Inoue K, Komura D, Kitakami J, Oshida N, Papantonis A, Izumi A, Kobayashi M, Meguro H, Kanki Y, Mimura I, Yamamoto K, Mataki C, Hamakubo T, Shirahige K, Aburatani H, Kimura H, Kodama T, Cook PR, Ihara S
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Journal Title
Proc Natl Acad Sci U S A
Volume: 106(43)
Pages: 18357-18361
DOI
Peer Reviewed
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[Journal Article] The peroxisomeproliferator-activated receptor gamma/retinoid X receptor alpha heterodimer targets the histonemodification enzyme PR-Set7/Setd8 gene and regulates adipogenesis through a positive feedback loop2009
Author(s)
Wakabayashi K, Okamura M, Tsutsumi S, Nishikawa NS, Tanaka T,Sakakibara I, Kitakami J, Ihara S,Hashimoto Y, Hamakubo T, Kodama T, Aburatani H, Sakai J
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Journal Title
Mol Cell Biol
Volume: 29(13)
Pages: 3544-3555
DOI
Peer Reviewed
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