2011 Fiscal Year Final Research Report
Structural basis for molecular mechanisms of substrate recognition and transport regulation by membrane transporters
| Project/Area Number |
20227003
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| Research Category |
Grant-in-Aid for Scientific Research (S)
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| Allocation Type | Single-year Grants |
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| Research Field |
Structural biochemistry
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| Research Institution | The University of Tokyo (2009-2012)
Tokyo Institute of Technology (2008) |
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Principal Investigator |
NUREKI Osamu 東京大学, 大学院・理学系研究科, 教授 (10272460)
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| Co-Investigator(Kenkyū-buntansha) |
MATURANA Andres 名古屋大学, 農学部, 准教授 (10452004)
ITO Koichi 東京大学, 新領域創成科学研究科, 教授 (10262073)
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| Project Period (FY) |
2008-05-12 – 2013-03-31
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| Keywords | X 線結晶構造解析 / チャネル / トランスポーター / 電気生理学解析 / 遺伝学解析 |
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| Research Abstract |
Membrane transporters maintain the intracellular circumstances by strictly regulating the import and export of metal ions, sugars, metabolites and drugs etc. To elucidate (A) how the transporters drive their transport, (B) how the transporters exclusively select their specific substrates and (C) how the transporters regulate their transporting activities at at an atomic resolution, we performed (1) structure determination by X-ray crystallography, (2) dynamic property analysis by MD simulation and (3) in vivo and in vitro complementary experiments, focusing on ion transporters of magnesium, iron and heavy metals, light-gated cation channels, protein transporters, peptide transporter and multidrug transporters. Especially, we uncovered the essential molecular mechanism of membrane transport by comparing and integrating the functional mechanisms of transporters specific for various kinds of target solutes.
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[Journal Article] Structure and function of a membrane component SecDF that enhances protein export2011
Author(s)
T.Tsukazaki,H.Mori,Y.Ech izen,R.Ishitani, S.Fukai,T.Tanaka,A.Perederina,D.G.Vassylyev, T.Koh no,A.D.Maturana, K.Itoand O.
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Journal Title
NurekiNature
Volume: 474
Pages: 235-238
Peer Reviewed
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[Journal Article] Tuberous Sclerosis Tumor Suppressor C omplex-like Complexes Act as GTPase-a ctivating Proteins for Ral GTPases2009
Author(s)
R. Shirakawa, S.Fukai, M.Kawato, T.Hi gashi, H. Kondo, T.Ikeda, E.Nakayam a, K.Okawa, O.Nureki, T.Kimura, T.Kita and H. Horiuchi.J
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Journal Title
Biol. Chem
Volume: 284
Pages: 21580-21588
Peer Reviewed
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