Molecular mechanism and regulation of assembly and remodeling of proteins

2010 Fiscal Year Self-evaluation Report

• Project/Area Number: 20227004
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Molecular biology
• Research Institution: National Institute of Genetics
• Principal Investigator: ARAKI Hiroyuki National Institute of Genetics, 細胞遺伝研究系, 教授 (20151160)
• Project Period (FY): 2008-05-12 – 2013-03-31
• Keywords: リモデリング / DNA複製 / 複製開始 / DNA合成

Research Abstract

細胞内で起こる生命現象の多くは、複数の因子が特定の時期に特定の場所に集合し、機能を発揮する反応である。遺伝情報を担う染色体DNAの複製においても、まずDNAを合成する酵素を含む多数のタンパク質が複製を開始するDNA領域に集合し、次にこれらタンパク質群が何らかの変換(リモデリング)をし、DNA合成を始める。本研究では、出芽酵母の染色体DNA複製開始過程をリモデリングのモデルとし、タンパク質の複製開始領域への集合とその後に起こるタンパク質複合体の変換(リモデリング)の分子機構、そして細胞周期によるこれらの調節機構の解明を目指す。そのため、複製開始領域へ集合するタンパク質群の挙動を細胞内で調べるとともに、これらタンパク質を精製し、タンパク質間の相互作用・複合体形成や、開始領域への集合を試験管内で詳細に調べる。これまでに、pre-LC複合体、Sld3-Sld7複合体を新たに同定し、これら複合体が複製開始領域への集合反応とその後のリモデリング反応に働くことを示すことができた。

Research Products

• [Journal Article] Sld7, an Sld3-associated protein required for efficient chromosomal DNA replication in budding yeast. (2011)
  • Author(s): Tanaka, T., Umemori, T., Endo, S., Muramatsu, S., Kanemaki, M., Kamimura, Y., Obuse, C., Araki, H.
  • Pages: 115(EMBO J.doi:10.1038/emboj.)
• [Journal Article] CDK-dependent complex formation between replication proteins (2010)
  • Author(s): Muramatsu, S., Hirai, K., Tak, Y-S., Kamimura, Y., Araki, H.
  • Journal Title: Dpbll, Sld2, Polε and GINS in budding yeast.Genes & Dev. 24 Pages: 602-612
  • Peer Reviewed
• [Journal Article] Cyclin-dependent kinase-dependent initiation of chromosomal DNA replication. (2010)
  • Author(s): Araki H.
  • Journal Title: Curt.Opin.Cell Biol. 22 Pages: 766-771
  • Peer Reviewed
• [Journal Article] Regulation of the initiation step of DNA replication by cyclin-dependent kinases. (2010)
  • Author(s): Tanaka, S., Araki, H.
  • Journal Title: Chromosoma 119 Pages: 565-574
  • Peer Reviewed
• [Journal Article] Regulatory mechanism of the initiation step of DNA replication by CDK in budding yeast. (2009)
  • Author(s): Araki H.
  • Journal Title: Biochimica et Biophysica Acta 1804 Pages: 520-523
  • Peer Reviewed
• [Presentation] Essential function of Pol epsilon at the initiation step of chromosomal DNA replication in budding yeast (2011)
  • Author(s): Araki, H., Tanaka, Y., Yanagisawa, Y., Endo, S., Hirai, K., Tanaka, S.
  • Organizer: Keystone Symposia "DNA Replication and Recombination"
  • Place of Presentation: Keystone, CO, USA.
  • Year and Date: 20110000
• [Presentation] How replication proteins associate with origins to initiate chromosomal DNA replication (2010)
  • Author(s): Araki H., Tanaka, T., Tanaka, Y., Yanagisawa, Y., Endo, S., Hirai, K., Tanaka, S.
  • Organizer: The 7th 3R Symposium
  • Place of Presentation: Toyama, Japan.
  • Year and Date: 20100000
• [Presentation] How replication proteins associate with and dissociate from origins to initiate chromosomal DNA replication (2010)
  • Author(s): Araki H., Tanaka, T., Tanaka, Y., Yanagisawa, Y., Endo, S., Tanaka, S.
  • Organizer: FASEB Summer Research Conferences "Yeast Chromosome Structure, Replication & Segregation"
  • Place of Presentation: Carefree, AZ, USA.
  • Year and Date: 20100000
• [Presentation] CDK-dependent assembly of replication proteins at the initiation step of chromosomal DNA replication (2009)
  • Author(s): Araki H., Hirai, K., Li, Y., Tanaka, T., Muramatsu, S., Tanaka, S.
  • Organizer: Eukaryotic DNA Replication & Genome Maintenance
  • Place of Presentation: Cold Spring Harbor, NY, USA.
  • Year and Date: 20090000
• [Presentation] Molecular mechanism of the initiation step of chromosomal DNA replication in budding yeast (2008)
  • Author(s): Araki H., Tanaka, T, Hirai, K., Tanaka, Y., Ememori, T., Yanagisawa, Y., Muramatsu, S., Tanaka, S.
  • Organizer: XX International Congress of Genetics
  • Place of Presentation: Berlin, Germany.
  • Year and Date: 20080000
• [Remarks] ホームページ
  • URL: http://www.nig.ac.jp/section/araki/araki-j.html