Molecular mechanism and regulation of assembly and remodeling of proteins

2013 Fiscal Year Final Research Report

• Project/Area Number: 20227004
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Molecular biology
• Research Institution: National Institute of Genetics
• Principal Investigator: ARAKI Hiroyuki 国立遺伝学研究所, 細胞遺伝研究系, 教授 (20151160)
• Project Period (FY): 2008-05-12 – 2013-03-31
• Keywords: リモデリング / DNA複製 / 複製開始 / DNA合成 / 細胞周期

Research Abstract

Many proteins assemble and form complexes at DNA replication origins. The complexes are subsequently remodeled to initiate DNA replication. This study newly identified the pre-LC and the Sld3-Sld7 protein complexes of budding yeast. These complexes recruit two replication factors, Cdc45 and GINS to origins and remodel replicative helicase, Mcm2-7 to an active form, which unwinds double-stranded DNA to single-stranded DNA to perform DNA replication.

Research Products

• [Journal Article] Concerted interaction between origin recognition complex (ORC), nucleosomes, and replication origin DNA ensures stable ORC-origin binding (2013)
  • Author(s): Hizume, K., Yagura, M. and Araki, H.
  • Journal Title: Genes Cells Volume: 18 Pages: 764-779
  • DOI: 10.1111/gtc.12073
  • Peer Reviewed
• [Journal Article] Kinetochores coordinate pericentromeric cohesion and early DNA replication by Cdc7-Dbf4 kinase recruitment (2013)
  • Author(s): Natsume, T., Muller, C. A., Katou, Y., Retkute, R., Gierliński, M., Araki, H., Blow, J.J., Shirahige, K., Nieduszynski, C.A. and Tanaka, T.U.
  • Journal Title: Mol. Cell Volume: 50 Pages: 661-674
  • DOI: 10.1016/j.molcel.2013.05.011
  • Peer Reviewed
• [Journal Article] Efficient initiation of DNA replication in eukaryotes requires Dpb11/TopBP1-GINS interaction (2013)
  • Author(s): Tanaka, S., Komeda, Y., Umemori, T., Kubota, Y., Takisawa, H. and Araki, H.
  • Journal Title: Mol. Cell. Biol Volume: 33 Pages: 2614-2622
  • DOI: 10.1128/MCB.00431-13
  • Peer Reviewed
• [Journal Article] Origin association of Sld3, Sld7, and Cdc45 proteins is a key step for determination of origin-firing timing (2011)
  • Author(s): Tanaka, S., Nakano, R., Katou, Y., Shirahige, K. and Araki, H.
  • Journal Title: Curr. Biol Volume: 21 Pages: 2055-2063
  • DOI: 10.1016/j.cub.2011.11.038
  • Peer Reviewed
• [Journal Article] Initiation of chromosomal DNA replication in eukaryotic cells; contribution of yeast genetics to the elucidation (2011)
  • Author(s): Araki, H
  • Journal Title: Genes Genet. Syst Volume: 86 Pages: 141-149
  • DOI: 10.1266/ggs.86.141
  • Peer Reviewed
• [Journal Article] Multiple regulatory mechanisms to inhibit untimely initiation of DNA replication are important for stable genome maintenance (2011)
  • Author(s): Tanaka, S. and Araki, H
  • Journal Title: PLoS Genet Volume: 7 Pages: e1002136 (1-16)
  • DOI: 10.1371/journal.pgen.1002136
• [Journal Article] Sld7, an Sld3-associated protein required for efficient chromosomal DNA replication in budding yeast (2011)
  • Author(s): Tanaka, T., Umemori, T., Endo, S., Muramatsu, S., Kanemaki, M., Kamimura, Y., Obuse, C. and Araki, H
  • Journal Title: EMBO J Volume: 30 Pages: 2019-2030
  • DOI: 10.1038/emboj.2011.115
• [Journal Article] CDK-dependent complex formation between replication proteins, Dpb11, Sld2, Polε and GINS in budding yeast (2010)
  • Author(s): Muramatsu, S., Hirai, K., Tak, Y-S., Kamimura, Y. and Araki, H
  • Journal Title: Genes & Dev Volume: 24 Pages: 602-612
  • DOI: 10.1101/gad.1883410
• [Journal Article] Cyclin-dependent kinase-dependent initiation of chromosomal DNA replication (2010)
  • Author(s): Araki, H
  • Journal Title: Curr. Opin. Cell Biol Volume: 22 Pages: 766-771
  • DOI: 10.1016/j.ceb.2010.07.015
• [Journal Article] Regulation of the initiation step of DNA replication by cyclin-dependent kinases (2010)
  • Author(s): Tanaka, S. and Araki, H
  • Journal Title: Chromosoma Volume: 119 Pages: 565-574
  • DOI: 10.1007/s00412-010-0291-8
• [Journal Article] Regulatory mechanism of the initiation step of DNA replication by CDK in budding yeast (2009)
  • Author(s): Araki, H
  • Journal Title: Biochimica et Biophysica Acta Volume: 1804 Pages: 520-523
  • DOI: 10.1016/j.bbapap.2009.10.020
• [Presentation] Formation of active replicative helicase in budding yeast (2013)
  • Author(s): Araki, H., Yagura, M., Makino, N., Tanaka, Y., Itou, H., Hizume, K. and Tanaka S
  • Organizer: 2013 Cold Spring Harbor Laboratory Meeting on Eukaryotic DNA Replication & Genome Maintenance
  • Place of Presentation: Cold Spring Harbor, NY, USA
  • Year and Date: 2013-09-11
• [Presentation] Establishment of replication forks at origins, Genome Instability (2013)
  • Author(s): Araki, H.
  • Organizer: Evolution and Human Diseases
  • Place of Presentation: St. Petersburg, Russia
  • Year and Date: 2013-06-15
• [Presentation] Molecular mechanism of initiation of chromosomal DNA replication (2013)
  • Author(s): Araki, H., Makino, N., Yagura, M., Tanaka, Y., Hizume, K. and Tanaka, S.
  • Organizer: Joint Conference of HGM 2013 and 21^<st> International Congress of Genetics
  • Place of Presentation: Singapore
  • Year and Date: 2013-04-13
• [Presentation] 出芽酵母染色体DNA の複製開始機構 (2012)
  • Author(s): 荒木弘之、牧野仁志穂、矢倉勝、田中尚美、遠藤静子、村松佐知子、日詰光治、田中誠司
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡市
  • Year and Date: 2012-12-13
• [Presentation] Molecular mechanism of initiation of chromosomal DNA replication in budding yeast (2012)
  • Author(s): Araki, H.
  • Organizer: The 8^<th> 3R Symposium
  • Place of Presentation: Awaji, Japan
  • Year and Date: 2012-11-27
• [Presentation] Initiation mechanism of chromosomal DNA replication (2012)
  • Author(s): Araki, H.
  • Organizer: BSCB/BSDB/JSDB-Joint Spring Meeting
  • Place of Presentation: Warwick, UK
  • Year and Date: 2012-04-16
• [Presentation] Essential role of DNA polymerase epsilon at the initiation step of chromosomal DNA replication in budding yeast (2011)
  • Author(s): Araki, H., Tanaka, Y., Yanagisawa, Y. and Endo, S.
  • Organizer: 2011 Cold Spring Harbor Laboratory Meeting on Eukaryotic DNA Replication & Genome Maintenance
  • Place of Presentation: Cold Spring Harbor, NY, USA
  • Year and Date: 2011-09-08
• [Presentation] Essential function of Pol epsilon at the initiation step of chromosomal DNA replication in budding yeast (2011)
  • Author(s): Araki, H., Tanaka, Y., Yanagisawa, Y., Endo, S., Hirai, K. and Tanaka, S.
  • Organizer: Keystone Symposia "DNA Replication and Recombination"
  • Place of Presentation: Keystone, CO, USA
  • Year and Date: 2011-03-03
• [Presentation] How replication proteins associate with origins to initiate chromosomal DNA replication (2010)
  • Author(s): Araki, H., Tanaka, T., Tanaka, Y., Yanagisawa, Y., Endo, S., Hirai, K. and Tanaka, S
  • Organizer: The 7th 3R Symposium
  • Place of Presentation: Toyama, Japan
  • Year and Date: 2010-10-30
• [Presentation] How replication proteins associate with and dissociate from origins to initiate chromosomal DNA replication (2010)
  • Author(s): Araki, H., Tanaka, T., Tanaka, Y., Yanagisawa, Y., Endo, S., and Tanaka, S
  • Organizer: FASEB Summer Research Conferences "Yeast Chromosome Structure, Replication & Segregation"
  • Place of Presentation: Carefree, AZ, USA
  • Year and Date: 2010-08-12
• [Presentation] CDK-dependent assembly of replication proteins at the initiation step of chromosomal DNA replication (2009)
  • Author(s): Araki, H., Hirai, K., Li, Y., Tanaka, T., Muramatsu, S., Tanaka, S
  • Organizer: 2009 Cold Spring Harbor Laboratory Meeting on Eukaryotic DNA Replication & Genome Maintenance
  • Place of Presentation: Cold Spring Harbor, NY, USA
  • Year and Date: 2009-09-01
• [Presentation] Molecular mechanism of the initiation step of chromosomal DNA replication in budding yeast (2008)
  • Author(s): Araki, H., Tanaka, T, Hirai, K., Tanaka, Y., Umemori, T., Yanagisawa, Y., Muramatsu, S. and Tanaka, S
  • Organizer: XX International Congress of Genetics
  • Place of Presentation: Berlin, Germany
  • Year and Date: 2008-07-13
• [Book] Helicase activation and establishment of replication forks at chromosomal origins of replication. In DNA replication (eds. Bell, S. D., Mechali, M., and DePamphilis M. L.) (2013)
  • Author(s): Tanaka, S. and Araki, H
  • Total Pages: 81-94,総頁数 561.
  • Publisher: Cold Spring Harbor Press, NY
• [Remarks]
  • URL: http://www.nig.ac.jp/section/araki/araki-j.html