Molecular mechanisms underlying cell polarization in migrating cells and neurons

2010 Fiscal Year Self-evaluation Report

• Project/Area Number: 20227006
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Cell biology
• Research Institution: Nagoya University
• Principal Investigator: KAIBUCHI Kozo Nagoya University, 医学系研究科, 教授 (00169377)
• Project Period (FY): 2008 – 2012
• Keywords: シグナル伝達 / 遊走 / 細胞骨格 / 極性 / プロテオーム / 微小管 / 脳・神経 / 小胞輸送

Research Abstract

生体を構成する種々の細胞は特徴的な極性を獲得し固有の生理機能を担っている。遊走する細胞、神経細胞や上皮細胞がその顕著な例である。細胞極性の獲得と維持は生体機能に必須であるものの、その分子基盤には不明の点も多い。本研究では、(1)遊走細胞と(2)神経細胞をモデルシステムとし、細胞極性の獲得・維持機構を制御するシグナル伝達機構の解明を行う。また、細胞極性の形成に関与する細胞骨格と選択的蛋白質・小胞輸送の制御機構の解明を目指す。さらには、(3)両システムに関連したプロテオミクス解析やシミュレーションモデルの作成により包括的な理解を目指す。

Research Products

• [Journal Article] Switching DISC1 function in neurogenesis : Dixdc1 selects DISC1 binding partners. (2010)
  • Author(s): Namba, T., Kaibuchi, K.
  • Journal Title: Dev Cell 19 Pages: 7-8
  • Peer Reviewed
• [Journal Article] A proteomic approach for comprehensively screening substrates of protein kinases such as Rho-kinase. (2010)
  • Author(s): Amano, M., Tsumura, Y., Taki, K., Harada, H., Mori, K., Nishioka, T., Kato, K., Suzuki, T., Nishioka, Y., Iwamatsu, A., Kaibuchi, K.
  • Journal Title: PLoS ONE 5 Pages: e8704
  • Peer Reviewed
• [Journal Article] Phosphorylation of CLASP2 by GSK-3beta regulates its interaction with IQGAP1, EB1 and microtubules. (2009)
  • Author(s): Watanabe, T., Noritake, J., Kakeno, M., Matsui, T., Harada, T., Wang, S., Itoh, N., Sato, K., Matsuzawa, K., Iwamatsu, A., Galjart, N., Kaibuchi, K.
  • Journal Title: Journal of Cell Science 122 Pages: 2969-2979
  • Peer Reviewed
• [Journal Article] Rho-kinase Contributes to Sustained RhoA Activation through Phosphorylation of p190A RhoGAP. (2009)
  • Author(s): Mori, K., Amano, M., Takefuji, M., Kato, K., Morita, Y., Nishioka, T., Matsuura, Y., Murohara, T., Kaibuchi, K.
  • Journal Title: The Journal of Biological Chemistry 284 Pages: 5067-5076
  • Peer Reviewed
• [Journal Article] Roles of disrupted-in-schizophrenia 1-interacting protein girdin in postnatal development of the dentate gyrus. (2009)
  • Author(s): Enomoto, A., Asai, N., Namba, T., Wang, Y., Kato, T., Tanaka, M., Tatsumi, H., Taya, S., Tsuboi, D., Kuroda, K., Kaneko, N., Sawamoto, K., Miyamoto, R., Jijiwa, M., Murakumo, Y., Sokabe, M., Seki, T., Kaibuchi, K., Takahashi, M.
  • Journal Title: Neuron 63 Pages: 774-787
  • Peer Reviewed
• [Journal Article] Anterograde transport of TrkB in axons is mediated by direct interaction with Slp1 and Rab27. (2009)
  • Author(s): Arimura, N., Kimura, T., Nakamuta, S., Taya, S., Funahashi, Y., Hattori, A., Shimada, A., Menager, C., Kawabata, S., Fujii, K., Iwamatsu, A., Segal, R.A., Fukuda, M., Kaibuchi, K.
  • Journal Title: Dev Cell 16 Pages: 675-686
  • Peer Reviewed
• [Presentation] Neuronal polarity-From extracellular signals to intracellular mechanisms. (2010)
  • Author(s): Kaibuchi K.
  • Organizer: Cold Spring Harbor Asia Conference, 1st Francis Crick Neuroscience Symposium
  • Place of Presentation: Suzhou, China
  • Year and Date: 2010-04-13
• [Presentation] Role of Par and Tiam1 complex in polarized cell migration. (2009)
  • Author(s): Kaibuchi K.
  • Organizer: RAMIC, National Network on Cell Adhesion and Migration
  • Place of Presentation: Madrid, Spain
  • Year and Date: 2009-10-22
• [Presentation] Roles of Rho family GTPases and Par proteins in directional migration. (2009)
  • Author(s): Kaibuchi K.
  • Organizer: Gordon Research Conferences
  • Place of Presentation: Oxford, UK
  • Year and Date: 2009-08-24
• [Presentation] Axon formation and polarized transport. (2009)
  • Author(s): Kaibuchi K.
  • Organizer: 第52回日本神経化学会大会
  • Place of Presentation: 渋川
  • Year and Date: 2009-06-22
• [Presentation] Axon formation and polarized vesicle transport. (2009)
  • Author(s): Kaibuchi K.
  • Organizer: ESF-EMBO Symposium
  • Place of Presentation: Sant Feliu de Guixols, Spain
  • Year and Date: 2009-05-23
• [Presentation] 細胞遊走におけるRhoファミリーとPar複合体の役割 (2008)
  • Author(s): 貝淵弘三
  • Organizer: 第60回日本細胞生物学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2008-06-30
• [Book] Cold Spring Harbor perspectives in biology 1(Cadherin-mediated Intercellular Adhesion and Signaling Cascades Involving Small GTPases.) (2009)
  • Author(s): Watanabe, T., Sato, K., Kaibuchi, K.
  • Total Pages: a003020
• [Remarks] ホームページ
  • URL: http://www.med.nagoya-u.ac.jp/Yakuri/