Structure and dynamics of actin filament complex:mechanism of calcium regulation of muscle contraction

2012 Fiscal Year Final Research Report

• Project/Area Number: 20227008
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Biophysics
• Research Institution: Nagoya University
• Principal Investigator: MAEDA Yuichiro 名古屋大学, 理学研究科, 特任教授 (10321811)
• Co-Investigator(Kenkyū-buntansha): NARITA Akihiro 名古屋大学, 理学研究科, 助教 (30360613)
• Co-Investigator(Renkei-kenkyūsha): KAINOSHO Masatsune 名古屋大学, 理学研究科, 研究顧問 (20137029) ; WATANABE Nobuhisa 名古屋大学, シンクロトロン光研究センター, 教授 (70212321)
• Project Period (FY): 2008 – 2012
• Keywords: 細胞運動 / 細胞骨格 / アクチン・トレッドミリング / 筋収縮の調節

Research Abstract

Actin is one of the most abundant proteins in eukaryotic cells, and preforms numerous crucial cellular functions. In muscle cells, actin monomers assemble into filamentous aggregates, play roles in muscle contraction and its regulation, while in non-muscle cells, actin drives cell motility through cycles of assembly and disassembly between monomers and filaments. In this project, the 3D structure of the actin filament was first elucidated, as well as structures of complex of actin with other proteins. Based on these structures, we have gained mechanistic understanding of actin cellular functions.

Research Products

• [Journal Article] Actin branching in the initiation and maintenance of lamellipodia. (2012)
  • Author(s): Vinzenz M., Nemethova M., Schur F., Mueller J., Narita A., Urban E., Winkler C., Schmeiser C., Koestler S. A., Rottner K., Resch G. P., Maeda Y. & Small, J. V
  • Journal Title: J Cell Sci Volume: 125 Pages: 2775-85
  • Peer Reviewed
• [Journal Article] Direct determination of actin polarity in the cell. (2012)
  • Author(s): Narita, A., Mueller, J., Urban, E., Vinzenz, M., Small, J. V. & Maeda, Y
  • Journal Title: J Mol Biol Volume: 419 Pages: 359-68
  • Peer Reviewed
• [Journal Article] Structural basis for the slow dynamics of the actin filament pointed end. (2011)
  • Author(s): Narita A, Oda T & Maeda Y
  • Journal Title: EMBO J Volume: 30 Pages: 1230-7
  • Peer Reviewed
• [Journal Article] Merits of the double-stranded form of the actin filament revealed by structures of the filament ends (2011)
  • Author(s): Narita, A
  • Journal Title: Commun Integr Biol Volume: 4 Pages: 692-5
  • Peer Reviewed
• [Journal Article] Minimum requirements for the actin-like treadmilling motor system. (2011)
  • Author(s): Narita, A
  • Journal Title: Bioarchitecture Volume: 1 Pages: 205-208
  • Peer Reviewed
• [Journal Article] Electron microscopic visualization of the filament binding mode of actin -binding proteins. (2011)
  • Author(s): Ito, T., Hirayama, T., Taki, M., Iyoshi, S., Dai, S., Takeda, S., Kimura-Sakiyama, C., Oda, T., Yamamoto, Y., Maeda, Y. & Narita, A.
  • Journal Title: filament binding mode of actin -binding proteins. J Mol Biol Volume: 408 Pages: 26-39
  • Peer Reviewed
• [Journal Article] Human spire interacts with the barbed end of the actin filament. (2011)
  • Author(s): Ito T, Narita A, Hirayama T, Taki M, Iyoshi, S., Yamamoto, Y., Maeda, Y. & Oda, T.
  • Journal Title: J Mol Biol Volume: 408 Pages: 18-25
  • Peer Reviewed
• [Journal Article] Two distinct mechanisms for actin capping protein regulation--steric and allosteric inhibition. (2010)
  • Author(s): Takeda, S., Minakata, S., Koike, R., Kawahata, I., Narita, A., Kitazawa, M., Ota, M., Yamakuni, T., Maeda, Y. & Nitanai, Y
  • Journal Title: PLoS Biol Volume: 8 Pages: e1000416
  • Peer Reviewed
• [Journal Article] Multiple Conformations of F-actin. (2010)
  • Author(s): Oda, T. & Maeda, Y
  • Journal Title: Structure Volume: 18 Pages: 761-7
  • Peer Reviewed
• [Journal Article] The nature of the globular- to fibrous-actin transition. (2009)
  • Author(s): Oda, T., Iwasa, M., Aihara, T., Maeda, Y. & Narita, A
  • Journal Title: Nature Volume: 457 Pages: 441-5
  • Peer Reviewed
• [Journal Article] Functional aberration of myofibrils by cardiomyopathy-causing mutations in the coiled-coil region of the troponin-core domain. (2009)
  • Author(s): Matsumoto, F., Maeda, K., Chatake, T., Maeda, Y. & Fujiwara, S.
  • Journal Title: Biochem Biophys Res Commun Volume: 382 Pages: 205-9
  • Peer Reviewed
• [Journal Article] Molecular structure of the ParM polymer and the mechanism leading to its nucleotide-driven dynamic instability. (2008)
  • Author(s): Popp, D., Narita, A., Oda, T., Fujisawa, T., Matsuo, H., Nitanai, Y., Iwasa, M., Maeda, K., Onishi, H. & Maeda, Y
  • Journal Title: EMBO J Volume: 27 Pages: 570-9
  • Peer Reviewed
• [Journal Article] Two-crystal structures of tropomyosin C-terminal fragment 176-273: exposure of the hydrophobic core to the solvent destabilizes the tropomyosin molecule (2008)
  • Author(s): Minakata, S., Maeda, K., Oda, N., Wakabayashi, K., Nitanai, Y. & Maeda, Y
  • Journal Title: Biophys J Volume: 95 Pages: 710-9
  • Peer Reviewed
• [Journal Article] Dual roles of Gln137 of actin revealed by recombinant human cardiac muscle alpha-actin mutants. (2008)
  • Author(s): Iwasa M, Maeda K, Narita A, Maeda Y & Oda T
  • Journal Title: J Biol Chem Volume: 283 Pages: 21045-53
  • Peer Reviewed
• [Presentation] Conformational and energetic cycle of actin in polymerization and depolymerization, EMBO-FEBS Lecture Course (2012)
  • Author(s): Maeda Y. Oda T., Narita A
  • Organizer: The 27th European Cytoskeleton Forum Meeting
  • Place of Presentation: Pecs, Hungary
  • Year and Date: 2012-11-04
• [Presentation] A Static View of the Dynamic Actin Filament (2011)
  • Author(s): Maeda Y
  • Organizer: Muscle & Molecular Motors at Gordon Research Conference
  • Place of Presentation: New London, NH., USA
  • Year and Date: 20110700
• [Presentation] Structural insights into mechanism of the actin treadmilling (2011)
  • Author(s): Maeda Y
  • Organizer: IX European Symposium of he Protein Society
  • Place of Presentation: Stockholm, Sweden
  • Year and Date: 20110500
• [Presentation] Two distinct mechanisms for actin capping protein regulation (2011)
  • Author(s): Takeda S
  • Organizer: Embo Lecture Courese The 26th. European Cytoskeletal Forum Meeting "Actin-Based Motility", Stresa
  • Place of Presentation: Lake Maggiore, Italy
  • Year and Date: 2011-11-10
• [Presentation] Single Particle analysis for actin filaments and other cytoskeletal filaments (2010)
  • Author(s): Narita A., Maeda Y
  • Organizer: The 66th. Annual Meeting of the Japanese Society of Microscopy Nagoya
  • Place of Presentation: Japan.
  • Year and Date: 20100500
• [Presentation] Two distinct mechanismss for actin capping protein regulation-Steric and allosteric infibition (2010)
  • Author(s): Maeda Y
  • Organizer: Alpbach meeting, Myosin Muscle and Many other motors
  • Place of Presentation: Alpbach, Austria
  • Year and Date: 20100300
• [Presentation] High resolution structure and basic properties of F-actin (2009)
  • Author(s): Maeda Y., Oda T., Narita A., Iwasa M., Maeda K., Aihara T
  • Organizer: International Union of Physiological Sciences(IUPS)
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 20090700
• [Presentation] Towards understanding the basic properties of F-actin (2009)
  • Author(s): Maeda Y
  • Organizer: Biochemical Interactions : from molecules to Function
  • Place of Presentation: Valen, Germany
  • Year and Date: 20090400
• [Presentation] The Nature of Gto F-actin transition (2008)
  • Author(s): Maeda Y, Oda T, Narita A
  • Organizer: Goradon Research Conference, "Muscle and molecular motors"
  • Place of Presentation: New London, NH, USA
  • Year and Date: 20080700
• [Book] 「細胞運動のメカニズム」、現代生物科学入門3,構造機能生物学、第二章 (2011)
  • Author(s): 前田雄一郎・小田俊郎
  • Publisher: 岩波書店
• [Remarks]
  • URL: http://str.bio.nagoya-u.ac.jp:8080/Plone