Dynamics of intrinsically disordered proteins and their functional roles

2010 Fiscal Year Self-evaluation Report

• Project/Area Number: 20227009
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Biophysics
• Research Institution: Yokohama City University
• Principal Investigator: NISHIMURA Yoshifumi Yokohama City University, 大学院・生命ナノシステム科学研究科, 教授 (70107390)
• Project Period (FY): 2008 – 2012
• Keywords: NMR / 転写因子 / クロマチン / 動的構造 / 天然変性状態

Research Abstract

本研究においては天然変性タンパク質の動的構造と機能制御機構の解明を行う。真核生物の特に核内タンパク質を標的に、単独では天然変性状態部位を持ち複合体形成に伴って全体としてフォールデイングするテロメア関連因子、ヒストンタンパク質、ヒストンシャペロン、クロマチン関連因子、基本転写因子を対象に、NMRを用いて、単独の時の動的構造、中間体の動的構造、特異的複合体の動的構造を解析し、天然変性タンパク質の認識機構の普遍性を解明する。

Research Products

• [Journal Article] Structural and bioche mical analyses of thehuman PAD4 var iant encoded by a functional haplot ype gene. (2011)
  • Author(s): Horikoshi, N., Tachiwana, H., Saito, K., Osakabe, A., Sato, M., Yamada, M., Akashi, S., Nishimura, Y., Kagawa, W., Kurumizaka, H.
  • Journal Title: Acta Crystal.D 67 Pages: 112-118
• [Journal Article] Deiminatio nstabilizes histone H2A/H2B dimers as revealed byelectrospray ionizati on mass spectrometry. (2010)
  • Author(s): Shimoyama, S.Nagadoi, A., Tachiwana, H., Yamada, M., Sato, M., Kurumizaka, H., Nishimura, Y., Akashi, S.
  • Journal Title: J.Mass.Spectrom 45 Pages: 900-908
• [Journal Article] Novel Structural and Functional Mode of a Knot Essential for RNA Binding Activity of the Esa1 Presumed Chromodomain. (2008)
  • Author(s): Shimojo, H., Sano, N., Moriwaki, Y., Okuda, M., Horikoshi, M., Nishimura, Y.
  • Journal Title: J.Mol.Biol. 378 Pages: 987-1001
• [Journal Article] Structural insight into the TFIIE/TFIIH interaction : TFIIE and p53 share the binding region on TFIIH. (2008)
  • Author(s): Okuda, M., Tanaka, A., Satoh, M., Mizuta, S., Takazawa, M., Ohkuma, Y., Nishimura, Y
  • Journal Title: EMBO J. 27 Pages: 1161-1171
• [Journal Article] Structural characterization of human general transcription factor TFIIF in solution. (2008)
  • Author(s): Akashi, S., Nagakura, S., Yamamoto, S., Okuda, M., Ohkuma, Y., Nishimura, Y.
  • Journal Title: Protein Sci. 17 Pages: 389-400
• [Presentation] NMR studies on gene regulation-related proteins : intrinsic disordered states and drug screening
  • Author(s): 西村善文
  • Organizer: Korean Magnetic Resonance Society Meeting開会講演
• [Remarks] ホームページ
  • URL: http://www.tsurumi.yokohama-cu.ac.jp/stbiol/index.html
• [Remarks]
  • URL: http://www.tsurumi.yokohama-cu.ac.jp/ynmr/
• [Remarks]
  • URL: http://www.tsurumi.yokohama-cu.ac.jp/segc/