2012 Fiscal Year Final Research Report
Dynamics of intrinsically disordered proteins and their functional roles
| Project/Area Number |
20227009
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| Research Category |
Grant-in-Aid for Scientific Research (S)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biophysics
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| Research Institution | Yokohama City University |
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Principal Investigator |
NISHIMURA Yoshifumi 横浜市立大学, 大学院・生命ナノシステム科学研究科, 教授 (70107390)
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| Co-Investigator(Kenkyū-buntansha) |
AKASHI Satoko 横浜市立大学, 大学院・生命ナノシステム科学研究科, 准教授 (10280728)
NAGADOI Aritaka 横浜市立大学, 大学院・生命ナノシステム科学研究科, 助教 (50336591)
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| Project Period (FY) |
2008 – 2012
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| Keywords | NMR / 転写因子 / クロマチン / 動的構造 / 天然変性状態 |
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| Research Abstract |
Here, we have examined dynamical structures of intrinsically disordered proteins including heterochromatin-related proteins, Chp1 and Swi6 in addition to histone multimers by NMR and MS. The chromodomain (CD) of a heterochromatin-related protein, Chp1, possesses novel RNA-binding activity as well as histone H3K9me3-binding activity. NMR revealed that the presence of a small helix in the N-terminal outside region of Chp1 CD bound to histone H3K9me3. Addition of cenRNA led to chemical shift changes of amide protons for residues within this helix and a C-terminal helix in Chp1 CD. In addition we revealed the solution structure of H2A/H2B and its complex with the C-terminal disordered acidic domain of histone chaperone Nap1.
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[Journal Article] Fission yeast Swi5-Sfr1 protein complex, an activator of Rad51 recombinase, forms an extremely elongated dogleg-shaped structure2011
Author(s)
Kokabu Y, Murayama Y, Kuwabara N, Oroguchi T, Hashimoto H, Tsutsui Y, Nozaki N, Akashi S, Unzai S, Shimizu T, Iwasaki H, Sato M, Ikeguchi M
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Journal Title
J Biol Chem
Volume: 286
Pages: 43569-43576
DOI
Peer Reviewed
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[Journal Article] The structure of the N-terminal regulatory domain of a plant NADPH oxidase and its functional implications2010
Author(s)
Oda T, Hashimoto H, Kuwabara N, Akashi S, Hayashi K, Kojima C, Wong HL, Kawasaki T, Shimamoto K, Sato M, Shimizu T
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Journal Title
J Biol Chem
Volume: 285
Pages: 1435-1445
Peer Reviewed
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[Journal Article] Guidelines for reporting the use of capillary electrophoresis in proteomics2010
Author(s)
Domann PJ, Akashi S, Barbas C, Huang L, Lau W, Legido-Quigley C, McClean S, Neususs C, Perrett D, Quaglia M, Rapp E, Smallshaw L, Smith NW, Smyth WF, Taylor CF
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Journal Title
Nat Biotechnol
Volume: 28
Pages: 654-655
DOI
Peer Reviewed
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