シグナルセンターとしての師部機能の解明

2010 Fiscal Year Annual Research Report

• Project/Area Number: 20247003
• Research Institution: The University of Tokyo
• Principal Investigator: 福田 裕穂 東京大学, 大学院・理学系研究科, 教授 (10165293)
• Keywords: 師部 / 維管束 / 細胞間シグナル / CLEペプチド / ブラシノステロイド / オーキシン / 形成層 / 受容体

Research Abstract

私たちは師部を中心にしたシグナル伝達系を明らかにすることで、師部とそのまわりの細胞との細胞間相互作用、ひいては植物の組織構築における細胞間相互作用の基本的原理を明らかにしようと考えた。本年度は以下のテーマについて研究を行うとともに、それをまとめた小冊子を作成した。
1.COE1(LRR-RLK)受容体の機能解析:COE1機能を知るために、連絡脈の間隔に異常をもっcoel変異体と野生型イネに対するオーキシンとブラシノステロイド添加実験を行った。また、ブラシノステロイド受容体に変異を持つd61変異体の解析と野生型イネへのブラシノステロイド添加実験を行った。これらの結果から、COE1は師部においてシグナルを受容し、その近傍での連絡脈形成の阻害を促進する。一方で、オーキシンシグナルとブラシノステロイドシグナルはその下流にあるCOE1を正に制御することで連絡脈の形成間隔を制御するというモデルを提示した。
2.CLE41機能の解明:12アミノ酸からなるペプチドシグナル分子TDIFをコードし師部特異的に発現する遺伝子の機能解析を行った。この遺伝子の突然変異体では、前形成層細胞で発現する受容体TDRの変異体と同じように前形成層異常という表現型を示したことから、師部でつくられるTDIFが細胞間シグナルとして前形成層細胞の発生運命を制御していることが遺伝学的に確かめられた。
3.師部形成に関与すると考えられるCLEペプチドの発現解析を行った。このCLEペプチドは木部に発現することが分かり、木部から師部へのシグナル系が師部分化に関与する可能性が示唆された。

Research Products

• [Journal Article] Mitogen-Activated Protein Kinase regulated by the CLAVATA receptors contributes to the shoot apical meristem homeostasis. (2011)
  • Author(s): Betsuyaku, S., Takahashi, F., Kinoshita, A., Miwa, H., Shinozaki, K., Fukuda, H., Sawa, S.
  • Journal Title: Plant Cell Physiol. Volume: 52 Pages: 14-29
  • Peer Reviewed
• [Journal Article] CLE peptides can negatively regulate protoxylem vessel formation via cytokinin signaling. (2011)
  • Author(s): Kondo, Y., Hirakawa, Y., Kieber, J., Fukuda, H.
  • Journal Title: Plant Cell Physiol. Volume: 52 Pages: 37-48
  • Peer Reviewed
• [Journal Article] ANGUSTIFOLIA3 plays roles in adaxial/abaxial patterning and growth in leaf morphogenesis. (2011)
  • Author(s): Horiguchi, G., Nakayama, H., Ishikawa N., Kubo, M., Demura, T., Fukuda, H., Tsukaya, H.
  • Journal Title: Plant Cell Physiol. Volume: 52 Pages: 112-124
  • Peer Reviewed
• [Journal Article] Areceptor-like kinase, KLAVIER, mediates systemic regulation of modulation and non-symbiotic shoot development in Lotus japonicus. (2011)
  • Author(s): Miyazawa, H., Oka-Kira, E., Sato, N., Takahashi, H., Wu, G.J., Sato, S., Hayashi, M., Betsuyaku, S., Nakazono, M., Tabata, S., Harada, Y., Sawa, S., Fukuda, H., Kawaguchi, M.
  • Journal Title: Development Volume: (in press)(掲載確定)
  • Peer Reviewed
• [Journal Article] Dynamics of Arabidopsis SUN proteins during mitosis and involvement in nuclear shaping. (2011)
  • Author(s): Oda, Y., H.Fukuda
  • Journal Title: Plant J. Volume: (in press)(掲載確定)
  • Peer Reviewed
• [Journal Article] CKHI/EER4 gene encoding a TAF12-like protein negatively regulates cytokinin sensitivity in Arabnidopsis thaliana. (2011)
  • Author(s): Kubo, M., Furuta, K., Demura, T., Fukuda, F., Liu, Y.-G., Shibata, S., Kakimoto, T.
  • Journal Title: Plant Cell Physiol. Volume: (in press)(掲載確定)
  • Peer Reviewed
• [Journal Article] Establishment and maintenance of vascular cell communities through local signaling. (2011)
  • Author(s): Hirakawa Y., Kondo, Y., Fukuda, H.
  • Journal Title: Curr.Opin.Plant Biol. Volume: 14 Pages: 17-23
  • Peer Reviewed
• [Journal Article] VND-INTERACTING2, a NAC Domain Protein, Negatively Regulates Xylem Vessel Formation in Arabidopsis. (2010)
  • Author(s): Yamaguchi, M., Ohtania, M., Mitsuda, N., Kubo, M., Ohme-Takagi, M., Fukuda, H., Demura, T.
  • Journal Title: Plant Cell Volume: 22 Pages: 1249-1263
  • Peer Reviewed
• [Journal Article] Wood cell-wall structure requires local 2D-microtubule disassembly by a novel plasma membrane-anchored microtubule-associated protein. (2010)
  • Author(s): Oda, Y., lida, Y., Kondo, Y., Fukuda H.
  • Journal Title: Curr.Biol. Volume: 20 Pages: 1197-1202
  • Peer Reviewed
• [Journal Article] COE1, an LRR-RLK responsible for commissral vein pattern formation in rice. (2010)
  • Author(s): Sakaguchi, J., Itoh, J.-I., Ito, Y., Nakamura, A., Fukuda, H., Sawa, S.
  • Journal Title: Plant Journal Volume: 63 Pages: 405-416
  • Peer Reviewed
• [Journal Article] TDIF peptide signaling regulates vascular stem cell proliferation via the wox4 homeobox gene in Arabidopsis. (2010)
  • Author(s): Hirakawa Y., Kondo, Y., Fukuda, H.
  • Journal Title: Plant Cell Volume: 22 Pages: 2618-2629
  • Peer Reviewed
• [Journal Article] Arabidopsis VASCULAR-RELATED NAC-DOMAIN6 directly regulates genes that govern programmed cell death and secondary wall formation in a coordinated way during xylem differentiation. (2010)
  • Author(s): Ohashi-Ito, K., Oda, Y., Fukuda, H.
  • Journal Title: Plant Cell Volume: 22 Pages: 3461-3473
  • Peer Reviewed
• [Journal Article] RPK2/TOAD2 is an essential receptor-like kinase transmitting the CLV3 signal in Arabidopsis. (2010)
  • Author(s): Kinoshita, A., Betsuyaku, S., Osakabe, Y., Mizuno, S., Nagawa, S., Stahl, Y., Simon, R., Yamaguchi-Shinozaki, K., Fukuda, H., Sawa, S.
  • Journal Title: Development Volume: 137 Pages: 3911-3920
  • Peer Reviewed
• [Journal Article] ADP-ribosylation factor machinery mediates endocytosis in plant cells. (2010)
  • Author(s): Naramoto, S., Kleine-Vehn, J., Robert, S., Fujimoto, M., Dainobu, T., Paciorek, T., Ueda, T., Nakano, A., Van Montagu, M.C.E., Fukuda, H., Friml, J.
  • Journal Title: Proc.Natl.Acad.Sci., USA Volume: 107 Pages: 21890-21895
  • Peer Reviewed
• [Journal Article] Regulation of vascular development by CLE peptide-receptor systems. (2010)
  • Author(s): Hirakawa Y., Kondo, Y., Fukuda, H.
  • Journal Title: J.Int.Plant Biol. Volume: 52 Pages: 8-16
  • Peer Reviewed
• [Journal Article] Transcriptional regulation of vascular cell fates. (2010)
  • Author(s): Ohashi-Ito, K., Fukuda, H.
  • Journal Title: Curr.Opin.Plant Biol. Volume: 13 Pages: 670-676
  • Peer Reviewed
• [Presentation] Maintenance of vascular stem cell activity by TDIF Peptide. (2010)
  • Author(s): Fukuda, H., Kondo, Y., Hirakawa, Y.
  • Organizer: Plant Science Communications 2010, 2^<nd> NIBB-MPIPZ symposium
  • Place of Presentation: Okazaki Conference Center, Japan
  • Year and Date: 20101116-20101118
• [Presentation] A novel membrane-associated protein regulating secondary wall patterns in an Arabidopsis cell culture harboring inducible VND6. (2010)
  • Author(s): Fukuda, H., Ohashi-Ito, K., Oda, Y.
  • Organizer: Cold Spring Harbor Asia Symposium "From Plant Biology to Crop Biotechnology"
  • Place of Presentation: CSH Asia, Suzhou, China
  • Year and Date: 20101025-20101029
• [Presentation] COE1, an LRR-RLK responsible for commissural vein pattern formation in rice. (2010)
  • Author(s): Sakaguchi, J., Itoh, J.-I., Ito, Y., Nakamura, A., Sawa, S., Fukuda, H.
  • Organizer: Cold Spring Harbor Asia Symposium "From Plant Biology to Crop Biotechnology"
  • Place of Presentation: CSH Asia, Suzhou, China
  • Year and Date: 20101025-20101029
• [Presentation] CLE peptides govern vascular stem cell fates. (2010)
  • Author(s): Fukuda, H., Kondo, Y., Hirakawa, Y.
  • Organizer: 8th International Conference on Plant Biology Frontiers : Cells and Signals
  • Place of Presentation: Wuyishan Yeohwa Resort, Wuyishan City, Fujian, China
  • Year and Date: 20100923-20100929
• [Presentation] 細胞膜ドメインに局在する新規微小管結合タンパク質MIDD1が二次細胞壁パターンを制御する (2010)
  • Author(s): 小田祥久、福田裕穂
  • Organizer: 日本植物学会第74回大会
  • Place of Presentation: 中部大学
  • Year and Date: 20100909-20100911
• [Presentation] CLEペプチドの成熟に関与するペプチダーゼの解析 (2010)
  • Author(s): 玉置貴之、別役重之、深尾陽一朗、藤原正幸、福田裕穂、澤進一郎
  • Organizer: 日本植物学会第74回大会
  • Place of Presentation: 中部大学
  • Year and Date: 20100909-20100911
• [Presentation] CLEペプチドとサイトカイニンによる原生木部配置パターンの制御 (2010)
  • Author(s): 近藤侑貴、平川有宇樹、福田裕穂
  • Organizer: 日本植物学会第74回大会
  • Place of Presentation: 中部大学
  • Year and Date: 20100909-20100911
• [Presentation] 維管束形成におけるTDIFシグナルによる幹細胞機能の制御 (2010)
  • Author(s): 平川有宇樹、近藤侑貴、福田裕穂
  • Organizer: 日本植物学会第74回大会
  • Place of Presentation: 中部大学
  • Year and Date: 20100909-20100911
• [Presentation] 維管束形成に関わる新規細胞間相互作用因子CLE46の解析 (2010)
  • Author(s): 大槻和弘、遠藤暁詩、伊藤容子、伊藤(大橋)恭子、福田裕穂
  • Organizer: 日本植物学会第74回大会
  • Place of Presentation: 中部大学
  • Year and Date: 20100909-20100911
• [Presentation] Clavata2(clv2)突然変異体のェンハンサースクリーニング (2010)
  • Author(s): 山田昌史, 福田裕穂, 澤進一郎
  • Organizer: 日本植物学会第74回大会
  • Place of Presentation: 中部大学
  • Year and Date: 20100909-20100911
• [Presentation] Cellular and transcriptome analyses of xylem formation with Arabidopsis cell culture systems harboring inducible VND6 and SND1. (2010)
  • Author(s): Fukuda, H., Oda, Y., Ohashi-Ito, K.
  • Organizer: Plant Vascular Biology 2010
  • Place of Presentation: Ohio State University, Ohio, USA
  • Year and Date: 20100723-20100728
• [Presentation] CLE peptides regulating vascular stem cell fates. (2010)
  • Author(s): Fukuda, H., Kondo, Y, Hirakawa, Y.
  • Organizer: 21st International Conference on Arabidopsis Research
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 20100606-20100610
• [Presentation] Screening of the CLV2 enhancer mutants. (2010)
  • Author(s): Yamada, M., Fukuda, H., Sawa, S.
  • Organizer: 21st International Conference on Arabidopsis Research
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 20100606-20100610
• [Presentation] Analysis of downstream genes regulated by master regulators of vascular differentiation. (2010)
  • Author(s): Ohashi-Ito, K., Oda, Y., Fukuda, H.
  • Organizer: 21st International Conference on Arabidopsis Research
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 20100606-20100610
• [Presentation] Regulation of auxin transport dependent leaf vascular continuity by VAN3 ARF-GAP. (2010)
  • Author(s): Naramoto, S, Koizumi, K., Ueda, T., Nakano, A., Fukuda, H., Friml, J.
  • Organizer: 21st International Conference on Arabidopsis Research
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 20100606-20100610
• [Presentation] Molecular dissection of CLV3 signaling. (2010)
  • Author(s): Betsuyaku, S., Kinoshita, A., Fujiwara, M., Fukao, Y., Kuromori, T., Shinozaki, K., Suzuki, A., Takahashi, H., Fukuda, H., Sawa, S.
  • Organizer: 21st International Conference on Arabidopsis Research
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 20100606-20100610
• [Presentation] Genetic analysis of the CLV signaling pathway in the SAM. (2010)
  • Author(s): Kinoshita, A., Betsuyaku, S., Yamada, M., Fukuda, H., Sawa, S.
  • Organizer: 21st International Conference on Arabidopsis Research
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 20100606-20100610
• [Presentation] TDIF peptide signaling regulates vascular stem cell proliferation via the WOX4 homeobox gene in Arabidopsis. (2010)
  • Author(s): Hirakawa, Y., Kondo, Y., FukudaH.
  • Organizer: 21st International Conference on Arabidopsis Research
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 20100606-20100610
• [Presentation] Wood cell-wall structure requires local 2d-microtubule disassembly by a novel plasma membrane-anchored microtubule-associated protein. (2010)
  • Author(s): Oda, Y., Fukuda, H.
  • Organizer: 21st International Conference on Arabidopsis Research
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 20100606-20100610
• [Presentation] VND-INTERACTING2, a NAC domain transcription factor, negatively regulates xylem vessel formation in Arabidopsis. (2010)
  • Author(s): Yamaguchi, M., Ohtani, M., Mitsuda, N., Kubo, M.Ohme-Takagi, M., Fukuda, H., Demura, T.
  • Organizer: 21st International Conference on Arabidopsis Research
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 20100606-20100610
• [Presentation] CLE peptides governing vascular stem cell fates. (2010)
  • Author(s): Fukuda, H., Kondo, Y., Hirakawa, Y.
  • Organizer: A seminar in The University of Michigan
  • Place of Presentation: Ann Arbor, Michigan, USA
  • Year and Date: 2010-07-29
• [Book] Plant tissue cultures. In"Methods in Molecular Biology : The Plant Cell Wall"(eds.ZA.Popper), vol.715 (2011)
  • Author(s): Karkonen, A., Santanen, A., IwamotoK., Fukuda, H.
  • Total Pages: 1-20
  • Publisher: Springer
• [Book] Plant tracheary elements. In"Encyclopedia of Life Sciences" (2010)
  • Author(s): Fukuda, H.
  • Total Pages: DOI:10.1002/9780470015902.a0001814.pub2
  • Publisher: John Wiley&Sons, Inc.(Wiley Online Library)
• [Book] CLEペプチドによる維管束形成の制御(柿本、高山、福田、松岡編)(植物のシグナル伝達-分子と応答) (2010)
  • Author(s): 平川有宇樹、近藤侑貴、福田裕穂
  • Total Pages: 172-177
  • Publisher: 共立出版
• [Book] 植物寄生性線虫がもつ植物ペプチドホルモン様遺伝子の機能とは,遺伝,vol.64 (2010)
  • Author(s): 別役重之、澤進一郎、福田裕穂
  • Total Pages: 52-59
  • Publisher: エヌ・エス・ティ
• [Remarks]
  • URL: http://www.biol.s.u-tokyo.ac.jp/users/seigyo/lab.html