Secretion mechanism of pathogenic proteases and adhesions in the periodontal pathogen Porphyromonas gingivalis.

2010 Fiscal Year Final Research Report

• Project/Area Number: 20249073
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Nagasaki University
• Principal Investigator: NAKAYAMA Koji Nagasaki University, 大学院医・歯薬学総合研究科, 教授 (80150473)
• Co-Investigator(Renkei-kenkyūsha): NAITO Mariko 長崎大学, 大学院・医歯薬学総合研究科, 准教授 (20244072) ; SHOJI Mikio 長崎大学, 大学院・医歯薬学総合研究科, 助教 (10336175) ; SATO Keiko 長崎大学, 大学院・医歯薬学総合研究科, 助教 (70410579) ; YUKITAKE Hideharu 長崎大学, 大学院・医歯薬学総合研究科, 技術職員 (30380984) ; HIRAKAWA Hideki かずさDNA研究所, 植物ゲノム情報研究室, 研究員 (80372746)
• Project Period (FY): 2008 – 2010

Research Abstract

Porphyromonas gingivalis secretes strong proteases called gingipains that are implicated in periodontal pathogenesis. Comparative genome analysis and genetic experiments revealed proteins involved in gingipain secretion. These proteins were similar in sequence to Flavobacterium johnsoniae gliding motility proteins. Disruption of the F. johnsoniae porT ortholog resulted in defects in motility and chitinase secretion, providing a link between a unique protein translocation system and a motility apparatus in members of the Bacteroidetes phylum.

Research Products

• [Journal Article] Effects of non-iron metalloporphyrins on growth and gene expression of Porphyromonas gingivalis. (2011)
  • Author(s): Yukitake H, Naito M, Sato K, Shoji M, Ohara N, Yoshimura M, Sakai E, Nakayama K
  • Journal Title: Microbiol Immunol 55 Pages: 141-53
• [Journal Article] A protein secretion system linked to bacteroidete gliding motility and pathogenesis. (2010)
  • Author(s): Sato K, Naito M, Yukitake H, Hirakawa H, Shoji M, McBride MJ, Rhodes RG, Nakayama K
  • Journal Title: Proc Natl Acad Sci USA 107 Pages: 276-281
• [Journal Article] Porphyromonas gingivalis peptidoglycans induce excessive activation of the innate immune system in silkworm larvae. (2010)
  • Author(s): Ishii K, Hamamoto H, Imamura K, Adachi T, Shoji M, Nakayama K, Sekimizu K.
  • Journal Title: J Biol Chem 285 Pages: 33338-33347
• [Journal Article] Porphyromonas gingivalis mutant defective in a putative glycosyltransferase exhibits defective biosynthesis of polysaccharide portions of lipopolysaccharide, decreased gingipain activities, strong autoaggregation and increased biofilm formation. (2010)
  • Author(s): Yamaguchi M, Sato K, Yukitake H, Noiri Y, Ebisu S, Nakayama K
  • Journal Title: Infect Immun 78 Pages: 3801-3812
• [Journal Article] Tetratricopeptide repeat protein-associated proteins contribute to the virulence of Porphyromonas gingivalis. (2010)
  • Author(s): Kondo Y, Ohara N, Sato K, Yoshimura M, Yukitake H, Naito M, Fujiwara T, Nakayama K
  • Journal Title: Infect Immun 78 Pages: 2846-2856
• [Journal Article] Characterization of hemin-binding protein 35 (HBP35) in Porphyromonas gingivalis : its cellular distribution, thioredoxin activity and role in heme utilization. (2010)
  • Author(s): Shoji M, Shibata Y, Shiroza T, Yukitake H, Peng B, Chen Y-Y, Sato K, Naito M, Abiko Y, Reynolds EC, Nakayama K
  • Journal Title: BMC Microbiol 10(online journal) Pages: 152
• [Journal Article] Neutralization of toxic haem by Porphyromonas gingivalis haemoglobin receptor. (2010)
  • Author(s): Nhien NT, Huy NT, Naito M, Oida T, Uyen DT, Huang M, Kikuchi M, Harada S, Nakayama K, Hirayama K, Kamei K
  • Journal Title: J.Biochem. 147 Pages: 317-325
• [Journal Article] Recombinant Porphyromonas gingivalis FimA preproprotein expressed in Escherichia coli is lipidated and a mature/processed recombinant FimA protein forms a short filament in vitro. (2010)
  • Author(s): Shoji M, Yoshimura A, Yoshioka H, Takade A, Takuma Y, Yukitake H, Naito M, Hara Y, Yoshida S, Nakayama K
  • Journal Title: Can J Microbiol 56 Pages: 959-967
• [Journal Article] Hemmaglutinin/adhesion domains of Porphyromonas gingizvalis play key roles in coaggregation with Treponema denticola. (2010)
  • Author(s): Ito R, Ishihara K, Shoji M, Nakayama K, Okuda K.
  • Journal Title: FEMS Immunol Med Microbiol 60 Pages: 251-260
• [Journal Article] Porphyromonas gingivalis cell-induced hemagglutination and platelet aggregation. (2010)
  • Author(s): Nakayama K
  • Journal Title: Periodontol 2000 54 Pages: 45-52
• [Journal Article] Hydrogen sulfide production from cysteine and homocysteine by oral bacteria. (2009)
  • Author(s): Yoshida A, Yoshimura M, Ohara N, Yoshimura S, Nagashima S, Takehara T, Nakayama K
  • Journal Title: J Periodontol 80 Pages: 1845-1851
• [Journal Article] Identification of a gingipain-sensitive surface ligand of Porphyromonas gingivalis that induces TLR2- and TLR4-independent NFkB activation in CHO cells. (2009)
  • Author(s): Haruyama K, Yoshimura A, Naito M, Kishimoto M, Shoji M, Abiko Y, Hara Y, Nakayama K
  • Journal Title: Infect Immun 77 Pages: 4414-4420
• [Journal Article] Porphyromonas gingivalis mutant defective in a putative ECF sigma factor shows a mutator phenotype. (2009)
  • Author(s): Kikuchi Y, Ohara N, Ueda O, Hirai K, Shibata Y, Nakayama K, Fujimura S.
  • Journal Title: Oral Microbiol Immunol 24 Pages: 377-383
• [Journal Article] Distinct roles of long/short fimbriae and gingipains in homotypic biofilm development by Porphyromonas gingivalis. (2009)
  • Author(s): Kuboniwa M, Amano A, Hashino E, Yamamoto Y, Inaba H, Hamada N, Nakayama K, Tribble GD, Lamont RJ, Shizukuishi S
  • Journal Title: BMC Microbiol 9 Pages: 105-115
• [Journal Article] Lipopolysaccharide biosynthesis-related genes are required for colonial pigmentation of Porphyromonas gingivalis. (2009)
  • Author(s): Sato K, Kido N, Murakami Y, Hoover CI, Nakayama K, Yoshimura F
  • Journal Title: Microbiology-SGM 55 Pages: 1282-1293
• [Journal Article] Proteome analysis of Porphyromonas gingivalis cell s placed in a subcutaneous chamber of mice. (2008)
  • Author(s): Yoshimura M, Ohara N, Kondo Y, Shoji M, Okano S, Nakano Y, Abiko Y, Nakayama K
  • Journal Title: Oral Microbiol Immunol 23 Pages: 413-418
• [Journal Article] Determination of the genome sequence of Porphyromonas gingivalis strain ATCC33277 and genomic comparison with strain W83 revealed extensive genome rearrangements in P.gingivalis. (2008)
  • Author(s): Naito M, Hirakawa H, Yamashita A, Ohara N, Shoji M, Yukitake H, Nakayama K, Toh H, Yoshimura F, Kuhara S, Hattori M, Hayashi T, Nakayama K
  • Journal Title: DNA Res 15 Pages: 215-225
• [Journal Article] Functional expression of lysine-specific cysteine proteinase (Lys-gingipain) by a Porphyromonas gingivalis plasmid system. (2008)
  • Author(s): Ishida Y, Hu J-P, Sakai E, Kadowaki T, Yamamoto K, Tsukuba T, Nakayama K, Okamoto K
  • Journal Title: Arch Oral Biol 53 Pages: 538-544
• [Journal Article] Dual regulation of IL-8 production in human oral epithelial cells upon stimulation with gingipains from Porphyromonas gingivalls. (2008)
  • Author(s): Uehara A, Naito M, Imamura T, Potempa J, Travis J, Nakayama K, Takada H
  • Journal Title: J Med Microbiol 57 Pages: 500-507
• [Presentation] Genome analysis and a novel secretion system of the periodontal pathogen Porphyromonas gingivalis. (2010)
  • Author(s): 中山浩次
  • Organizer: 第3回日中科学フォーラム 招待講演
  • Place of Presentation: 武漢,中国
  • Year and Date: 20100000
• [Presentation] 比較ゲノム解析から発見されたグラム陰性細菌の新規タンパク分泌機構 (2010)
  • Author(s): 中山浩次
  • Organizer: 第4回日本ゲノム微生物学会年会 招待講演
  • Place of Presentation: 福岡
  • Year and Date: 20100000
• [Presentation] Secretion of Pathogenic Proteins in the Periodontopathic Bacterium Porphyromonas gingivalis. (2009)
  • Author(s): 中山浩次
  • Organizer: US-Japan Symposium 招待講演
  • Place of Presentation: Niigata, Japan.
  • Year and Date: 20090000
• [Presentation] Secretion of Pathogenic Proteins in Porphyromonas gingivalis. (2009)
  • Author(s): 中山浩次
  • Organizer: IADR Miami 招待講演
  • Place of Presentation: Florida, USA.
  • Year and Date: 20090000
• [Presentation] 歯周病細菌Porphyromonas gingivalisのpathology. (2009)
  • Author(s): 中山浩次
  • Organizer: 第52回日本歯周病学会春季学術大会 招待講演
  • Place of Presentation: 岡山
  • Year and Date: 20090000
• [Book] Genomics of Porphyromonas gingivalis.(Periodontal Mediclrle and Systems Biology, chapter 13) (2009)
  • Author(s): Nakayama K
  • Total Pages: 193-204
  • Publisher: Wiley-Blackwell
• [Remarks] 長崎大学歯学部歯周病基盤研究センターホームページ
  • URL: http://www.de.nagasaki-u.ac.jp/index_j.html