2010 Fiscal Year Final Research Report
Improvement of high-throughput method to generate the knock-in ES
| Project/Area Number |
20310123
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied genomics
|
|---|
| Research Institution | Kazusa DNA Research Institute |
|---|
Principal Investigator |
NAKAYAMA Manabu Kazusa DNA Research Institute, ヒトゲノム研究部・ヒト遺伝子応用技術研究室, 主任研究員 (30370927)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Keywords | 遺伝子 / ゲノム / 機能ゲノム / 動物 / 発生・分化 / バイオテクノロジー / 病態モデル動物 |
|---|
| Research Abstract |
Producing of knock-in mouse is extremely useful method to analyze various proteins' function in mice. By using Red/ET recombination system which can be recombined any DNA sequence through homologous recombination reaction, we have developed a high-through system to construct targeting vectors for knock-in and a new system to introduce exchangeable protein tag to C-terminal end of the protein. We also have developed two new site-specific recombination systems to engineer genome of mouse ES cells.
|
-
-
-
-
-
-
-
-
-
-
[Journal Article] The zinc transporter SLC39A13/ZIP13 is required for connective tissue development ; its involvement in BMP/TGF-beta signaling pathways.2008
Author(s)
Fukada T, Civic N, Furuichi T, Shimoda S, Mishima K, Higashiyama H, Idaira Y, Asada Y, Kitamura H, Yamasaki S, Hojyo S, Nakayama M, Ohara O, Koseki H, Dos Santos HG, Bonafe L, Ha-Vinh R, Zankl A, Unger S, Kraenzlin ME, Beckmann JS, Saito I, Rivolta C, Ikegawa S, Superti-Furga A, Hirano T
-
Journal Title
PLoS ONE. Vol.3
Pages: e3642
Peer Reviewed
-
-
-
-
[Remarks] ホームページ等
-
-
