2010 Fiscal Year Final Research Report
MAGNETIC FORCE-BASED-DROPLET-HANDLING SYSTEM FOR SINGLE CELL DIGITAL PCR ANALYSIS
| Project/Area Number |
20360371
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Nagoya University |
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Principal Investigator |
OKOCHI Mina Nagoya University, 工学研究科, 准教授 (70313301)
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| Co-Investigator(Kenkyū-buntansha) |
HONDA Hiroyuki 名古屋大学, 大学院・工学研究科, 教授 (70209328)
KATO Ryuji 名古屋大学, 大学院・工学研究科, 助教 (50377884)
SHIKIDA Mitsuhiro 名古屋大学, 大学院・工学研究科, 准教授 (80273291)
MIZUTANI Takashi 名古屋大学, 大学院・工学研究科, 教授 (70273290)
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| Project Period (FY) |
2008 – 2010
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| Keywords | バイオセンサ / 一細胞解析 |
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| Research Abstract |
A droplet-based single cell analysis system was developed employing magnetic force-based droplet-handling system. The droplets containing magnetic beads were magnetically controlled and coalesced with another droplet in the oil phase, and all the reactions for RT-PCR including single cell manipulation, cell lysis, cDNA synthesis, and PCR amplification steps were integrated on-chip and applied for detection of cancer cells.
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Research Products
(41 results)
