2010 Fiscal Year Final Research Report

Whole-cell biocatalyst with non-natural membrane structure

Research Project

Project/Area Number	20360375
Research Category	Grant-in-Aid for Scientific Research (B)
Allocation Type	Single-year Grants
Section	一般
Research Field	Biofunction/Bioprocess
Research Institution	Kobe University
Principal Investigator	KONDO Akihiko Kobe University, 工学研究科, 教授 (40205547)
Co-Investigator(Kenkyū-buntansha)	TANAKA Tsutomu 神戸大学, 自然科学系・先端融合研究環重点研究部, 助教 (90436551)
Project Period (FY)	2008 – 2010
Keywords	タンパク質 / 酵素 / 非天然分子 / 酵母 / 細胞表層提示
Research Abstract	Introduction of non-natural, functional molecules on the cell surface was developed for establishment of several stress, such as organic medium containing broth. Using biotin-streptavidin interaction, non-natural molecules were successfully incorporated onto yeast cell surface. In addition, novel enzymes, which can conjugate some functional molecules on the cell surfaces, were explored. The evaluation of cell surface modified microorganisms' functions are undergoing such as stress tolerance.