Studies on recognition system of male and female gametes and cytoplasmic inheritance of organelles during fertilization and zygote development of algae

2010 Fiscal Year Final Research Report

• Project/Area Number: 20370025
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphology/Structure
• Research Institution: Hokkaido University
• Principal Investigator: MOTOMURA Taizo Hokkaido University, 北方生物圏フィールド科学センター, 教授 (30183974)
• Co-Investigator(Kenkyū-buntansha): KAWANO Shigeyuki 東京大学, 新領域創成科学研究科, 教授 (70161338) ; MIYAMURA Shin-Ichi 筑波大学, 生命環境科学研究科, 准教授 (00192766) ; KATO Atsushi 北海道大学, 大学院・理学研究院, 教授 (90177428) ; NAGASATO Chikako 北海道大学, 北方生物圏フィールド科学センター, 准教授 (00374710)
• Project Period (FY): 2008 – 2010
• Keywords: 藻類 / 褐藻 / 葉緑体 / ミトコンドリア / 細胞質遺伝 / オルガネラDNA

Research Abstract

Cytoplasmic inheritance of chloroplasts and mitochondria during fertilization of brown algae were studied by electron microscopy and molecular biological techniques using Scytosiphon lomentaria (isogamy), Unidaria pinnatifica and Cystoseira hakodatensis (oogamy). Chloroplasts were derived from both parents in S. lomentaria, while mitochondrial DNA derived from male gametes became selectively disappeared from four-celled sporophytes. In the case of oogamous U. pinnatifida, sperm chloroplasts and mitochondria were digested by lysosome soon after fertilization. In C. hakodatensis, differences of the organellar DNA methylation degrees could be clearly detected between eggs and sperm.

Research Products

• [Journal Article] Comparative immunofluorescence and ultrastructural analysis of microtubule organization in Uronema sp., Klebsormidium flaccidum, K. subtilissimum, Stichococcus bacillaris and S. chloranthus (Chlorophyta) (2011)
  • Author(s): Katsaros C, Varvarigos V., Gachon C. M. M., Brand, J., Motomura T., Nagasato C., Kupper F. C.
  • Journal Title: Protist 162 Pages: 315-331
• [Journal Article] Chlamydomonas reinhardtii : Duration of its cell cycle and phases at growth rates affected by light intensity (2011)
  • Author(s): Vitova M., Bi ov, K.; Umysov D., Hlavov M.; S., Kawano S. Zachleder V., Cizkova, M.
  • Journal Title: Planta 233 Pages: 75-86
• [Journal Article] Disappearance of male mitochondrial DNA after the 4-celled stage sporophyte of the isogamous brown alga Scytosiphon lomentaria (Scytosiphonales, Phaeophyceae). (2010)
  • Author(s): Kimura K., Nagasato C., Kogame K., Motomura T.
  • Journal Title: Journal of Phycology 46 Pages: 143-152
• [Journal Article] Cytoplasmic inheritance of organelles in brown algae. (2010)
  • Author(s): Motomura T., Nagasato C., Kimura K.
  • Journal Title: Journal of Plant Research 123 Pages: 185-192
• [Journal Article] Overexpression of the NAC transcription factor family gene ANAC036 results in a dwarf phenotype in Arabidopsis thaliana. (2010)
  • Author(s): Kato H, Motomura T., Komeda Y., Saito T., Kato A.
  • Journal Title: Journal of Plant Physiology 167 Pages: 571-577
• [Journal Article] 紅藻スサビノリの光合成色素と葉緑体微細構造における栄養欠乏応答 (2010)
  • Author(s): 植木知佳,村上明男,加藤敏朗,嵯峨直恆,本村泰三
  • Journal Title: 日本水産学会誌 76 Pages: 375-382
• [Journal Article] The Ectocarpus genome and the independent evolution of multicellularity in the brown algae (2010)
  • Author(s): J. Mark Cock, Lieven Sterck, Pierre Rouze, Delphine Scornet, Andrew E. Allen, Grigoris Amoutzias, Veronique Anthouard, Francois Artiguenave, Jean-Marc Aury, Jonathan H. Badger, Bank Beszteri, Kenny Billiau, Eric Bonnet, John H. F. Bothwell, Chris Bowler, Catherine Boyen, Colin Brownlee, Carl J. Carrano, Benedicte Charrier, Ga Youn Cho, Susana M. Coelho, Jonas Collen, Erwan Corre, Corinne Da Silva, Ludovic Delage, Nicolas Delaroque, Simon M. Dittami, Sylvie Doulbeau, Marek Elias, Garry Farnham, Claire M.M. Gachon, Bernhard Gschloessl, Svenja Heesch, Kamel Jabbari, Claire Jubin, Hiroshi Kawai, Kei Kimura, Bernard Kloareg, Frithjof C. Kupper, Daniel Lang, Aude Le Bail, Catherine Leblanc, Patrice Lerouge, Martin Lohr, Pascal J. Lopez, Cindy Martens, Florian Maumus, Gurvan Michel , Diego Miranda-Saavedra, Julia Morales, Herve Moreau, Taizo Motomura, Chikako Nagasato, Carolyn A. Napoli, David R. Nelson, Pi Nyvall-Collen, Akira F. Peters, Cyril Pommier, Philippe Potin, Julie Poulain, Hadi Quesneville, Betsy Read, Stefan A. Rensing, Andres Ritter, Sylvie Rousvoal, Manoj Samanta, Gaelle Samson, Declan C. Schroeder, Beatrice Segurens, Martina Strittmatter, Thierry Tonon, James Tregear, Klaus Valentin, Peter von Dassow, Takahiro Yamagishi, Yves Van de Peer, Patrick Wincker
  • Journal Title: Nature 465 Pages: 617-621
• [Journal Article] Analysis of expressed sequence tags from the chrysophycean alga Ochromonas danica (Heterokontophyta). (2010)
  • Author(s): Terauchi M., Kato A. Nagasato C, Motomrua T.
  • Journal Title: Phycological Research 58 Pages: 217-221
• [Journal Article] Membrane fusion process and assembly of cell wall during cytokinesis in the brown alga, Silvetia babingtonii (Fucales, Phaeophyceae) (2010)
  • Author(s): Nagasato C., Inoue A., Mizuno M., Kanazawa K. Ojima T., Kazuo Okuda, Motomrua T.
  • Journal Title: Planta 232 Pages: 287-298
• [Journal Article] Sperm mitochondrial DNA elimination in the zygote of the oogamous brown alga Undaria pinnatifida (Laminariale, Phaeophyceae) (2010)
  • Author(s): Kimura K., Nagasato C., Shinya U., Motomura T.
  • Journal Title: Cytologia 75 Pages: 353-361
• [Journal Article] Cytoplasmic inheritance in green algae : patterns, mechanisms and their relations with sex types. (2010)
  • Author(s): Miyamura S.
  • Journal Title: Journal of Plant Research 123 Pages: 171-184
• [Journal Article] Behavior of flagellar root systems in the planozygotes and settled zygotes of the green alga Bryopsis maxima Okamura (Ulvophyceae, Chlorophyta) with reference to spatial arrangement of eyespot and cell fusion site (2010)
  • Author(s): Miyamura S., Sakaushi S., Hori T., Nagumo T.
  • Journal Title: Phycological Research 58 Pages: 258-269
• [Journal Article] Isolation of chloroplast FtsZ and AtpC, and analysis of protein targeting into the complex chloroplast of the haptophyte Pavlova pinguis. (2010)
  • Author(s): Nishikawa T., Kajitani H., Sato M., Mogi Y., Moriyama Y., Kawano S.
  • Journal Title: Cytologia 75 Pages: 203-210
• [Journal Article] Chloroplasts divide by contraction of a bundle of nanofilaments consisting of polyglucan. (2010)
  • Author(s): Yoshida Y., Kuroiwa H., Misumi O., Yoshida M., Ohnuma M., Fujiwara T., Yagisawa F., Hirooka S., Imoto Y., Matsushita K., Kawano S., Kuroiwa T.
  • Journal Title: Science 329 Pages: 949-953
• [Journal Article] Ultrastructure of mitosis and cytokinesis during spematogenesis in Porphyra yezoensis (Bangiales, Rhodophyta). (2009)
  • Author(s): Ueki C., Nagasato C., Motomura T., Saga N.
  • Journal Title: Botanica Marina 52 Pages: 129-139
• [Journal Article] Functional and non-functional spindle formation affecting mitosis and cytokinesis in Fucus distichus zygotes : the role of the centrosome. (2009)
  • Author(s): Motomura T., Nagasato C.
  • Journal Title: Botanica Marina 52 Pages: 140-149
• [Journal Article] Diaphragm development in cytokinetic vegetative cells of brown algae. (2009)
  • Author(s): Katsaros C., Motomura T., Nagasato C., Galatis B.
  • Journal Title: Botanica Marina 52 Pages: 150-161
• [Journal Article] Effect of latrunculin B and brefeldin A on cytokinesis in the brown alga, Scytosiphon lomentaria (Scytosiphonales, Phaeophyceae). (2009)
  • Author(s): Nagasato C., Motomura T.
  • Journal Title: Journal of Phycology 45 Pages: 404-412
• [Journal Article] Distribution and phylogeny of the blue-light receptors aureochromes in eukaryotes. (2009)
  • Author(s): Ishikawa M., Takahashi F., Nozaki H., Nagasato C., Motomura T., Kataoka H.
  • Journal Title: Planta 230 Pages: 543-552
• [Journal Article] Novel proteins comprising the Stramenopile tripartite mastigoneme in Ochromonas danica (Chrisophyceae). (2009)
  • Author(s): Yamagishi T., Motomura T., Nagasato C., Kawai H.
  • Journal Title: Journal of Phycology 45 Pages: 1100-1105
• [Journal Article] Identification of photosynthetic sacoglossans from Japan. (2009)
  • Author(s): Yamamoto Y. Y., Yusa Y., Yamamoto S., Hirano Y., Hirano Y., Motomura T., Tanemura T., Obokata J.
  • Journal Title: Encocytobiosis Cell Research 19 Pages: 112-119
• [Journal Article] Visualizing the spatial arrangement of flagella-eyespots-cell fusion sites in gametes and planozygotes of Chlamydomonas reinhardtii (Chlorophyceae, Chlorophyta) with high-resolution FE-SEM. (2009)
  • Author(s): Miyamura S., Mogi Y., Mitsuhashi F., Kawano S., Nagumo T.
  • Journal Title: Cytologia 74 Pages: 409-415
• [Journal Article] The dynamic surface of dividing cyanelles and ultrastructure of the region directly below the surface in Cyanophora paradoxa. (2009)
  • Author(s): Sato M., Mogi Y., Nishikawa T., Miyamura S., Nagumo T., Kawano S.
  • Journal Title: Planta 229 Pages: 781-791
• [Journal Article] The bacterial ZapA-like protein ZED is required for mitochondrial division. (2009)
  • Author(s): Yoshida Y., Kuroiwa H., Hirooka S., Fujiwara T., Ohnuma M., Yoshida M., Misumi O., Kawano S., Kuroiwa T.
  • Journal Title: Current Biology 19 Pages: 1491-1497
• [Journal Article] Reexamination of the Pit Plugs and the Characteristic Membranous Structures in Porphyra Yezoensis (Bangiales, Rhodophyta). (2008)
  • Author(s): Ueki, C., Nagasato C., Motomura T., Saga N.
  • Journal Title: Phycologia 47 Pages: 5-11
• [Journal Article] Sexuality and uniparental inheritance of chloroplast DNA in the isogamous green alga Ulva compressa (Ulvophyceae). (2008)
  • Author(s): Kagami Y., Mogi Y., Arai T., Yamamoto M., Kuwano K., Kawano S.
  • Journal Title: Journal of Phycology 44 Pages: 691-702
• [Journal Article] Asymmetry of eyespot and mating structure positions in Ulva compressa (Ulvales, Chlorophyta) revealed by a new FE-SEM method. (2008)
  • Author(s): Mogi Y., Kagami Y., Kuwano K., Miyamura S., Nagumo T., Kawano S.
  • Journal Title: Journal of Phycology 44 Pages: 1290-1299
• [Journal Article] Isolation and characterization of microsatellites in the green alga Ulva compressa (Chlorophyta). (2008)
  • Author(s): Kagami Y., Arai T., Mogi Y., Kuwano K., Kawano S.
  • Journal Title: Cytologia 73 Pages: 387-392
• [Presentation] Cell structure of brown algae ; unicellular reproductive cells and multicellular organization. (2010)
  • Author(s): 本村泰三
  • Organizer: Ectocarpus 2010 : A decade of algal genomics (plenary lecture).
  • Place of Presentation: Ghent University, Belgium
  • Year and Date: 20100407-20100409
• [Presentation] Something you always wanted to know about sex-characterization of sex-related proteins of Scytoiphon lomentaria. (2010)
  • Author(s): Schmid D, Nagasato, C,. Motomura T., Boland W.
  • Organizer: Ectocarpus 2010 : A decade of algal genomics.
  • Place of Presentation: Ghent University, Belgium
  • Year and Date: 20100407-20100409
• [Presentation] Difference of male mitochondrial digestion and mitochondrial DNA elimination after fertilization between isogamous and oogamous brown algae. (2010)
  • Author(s): Kimurra K., Nagasato C., Motomura T.
  • Organizer: Ectocarpus 2010 : A decade of algal genomics.
  • Place of Presentation: Ghent University, Belgium
  • Year and Date: 20100407-20100409
• [Presentation] Electron tomographic analysis of cytokinesis in Silvetia babingtonii. (2010)
  • Author(s): Nagasato C., Kajimura N., Ueki C., Mineyuki Y., Motomura T.
  • Organizer: Ectocarpus 2010 : A decade of algal genomics.
  • Place of Presentation: Ghent University, Belgium
  • Year and Date: 20100407-20100409
• [Presentation] Ultrastructural study on plasmodesmata formed during cytokinesis in brown alga Dictyota dichotoma. (2010)
  • Author(s): Terauchi M., Kajimura N., Nagasato C., Katsaros C., Mineyuki Y., Motomura T.
  • Organizer: Ectocarpus 2010 : A decade of algal genomics.
  • Place of Presentation: Ghent University, Belgium
  • Year and Date: 20100407-20100409
• [Presentation] 電子線トモグラフィー法を用いた褐藻エゾイシゲの細胞質分裂における膜構造変化の解析 (2010)
  • Author(s): 長里千香子,梶村直子,植木知佳,峰雪芳宣,本村泰三
  • Organizer: 藻類学会第34回大会
  • Place of Presentation: 筑波大学,つくば
  • Year and Date: 20100319-20100322
• [Presentation] 褐藻アミジグサにおける細胞質分裂時に形成されるplasmodesmataの微細構造解析 (2010)
  • Author(s): 寺内真,梶村直子,長里千香子,Christos Katsaros,峰雪芳宣,本村泰三
  • Organizer: 藻類学会第34回大会
  • Place of Presentation: 筑波大学,つくば
  • Year and Date: 20100319-20100322
• [Presentation] 褐藻類の細胞質分裂における膜融合過程と細胞壁形成のタイミング (2009)
  • Author(s): 長里千香子,井上晶,尾島孝男,奥田一雄,本村泰三
  • Organizer: 日本植物学会第73回大会
  • Place of Presentation: 山形大学,山形市
  • Year and Date: 20090917-20090920
• [Presentation] Fertilization, zygote development and cell division in brown algae. (2009)
  • Author(s): Motomura T.
  • Organizer: Plenary lecture第9回国際藻類学会議
  • Place of Presentation: 代々木オリンピックセンター,東京
  • Year and Date: 20090802-20090808
• [Presentation] Membrane fusion during cytokinesis of brown algae, Fucus distichus and Silvetia babingtonii (Fucales, Phaeophyceae) (2009)
  • Author(s): Nagasato C., Inoue A., Ojima T., Okuda K., Motomura T.
  • Organizer: 第9回国際藻類学会議
  • Place of Presentation: 代々木オリンピックセンター,東京
  • Year and Date: 20090802-20090808
• [Presentation] Differences of elimination timinig in male mitochondria and mitochondrial DNA after fertilization between isogamous and oogamous brown algae. (2009)
  • Author(s): Kimura K., Kogame K. Uwai S., Nagasato C., Motomura T.
  • Organizer: 第9回国際藻類学会議
  • Place of Presentation: 代々木オリンピックセンター,東京
  • Year and Date: 20090802-20090808
• [Presentation] The EST Analysis and Fundamental Experiment for Gene transformation in Chrysophycean alga Ochromonas danica. (2009)
  • Author(s): Terauchi M., Kato A., Nagasato C., Motomura T.
  • Organizer: 第9回国際藻類学会議
  • Place of Presentation: 代々木オリンピックセンター,東京
  • Year and Date: 20090802-20090808
• [Presentation] Sex-related proteins in Scytosiphon lomentaria (Phaeophyceae)-a comparative proteomic approach. (2009)
  • Author(s): Schmid D., Nagasato C., Muck A., Loele A., Svatos A., Motomura T., Boland W.
  • Organizer: 第9回国際藻類学会議
  • Place of Presentation: 代々木オリンピックセンター,東京
  • Year and Date: 20090802-20090808
• [Presentation] 藻類の細胞質分裂時における隔膜発達と細胞壁沈着過程 (2009)
  • Author(s): 長里千香子,井上晶,尾島孝男,奥田一雄,本村泰三
  • Organizer: 藻類学会第33回大会
  • Place of Presentation: 琉球大学,沖縄
  • Year and Date: 20090326-20090329
• [Presentation] 褐藻ワカメの受精時におけるミトコンドリア,葉緑体の母性遺伝機構の解析 (2009)
  • Author(s): 木村圭,上井進也,長里千香子,本村泰三
  • Organizer: 藻類学会第33回大会
  • Place of Presentation: 琉球大学,沖縄
  • Year and Date: 20090326-20090329
• [Presentation] 黄金色藻オクロモナスにおける遺伝子導入系に向けてのEST解析と基礎実験 (2009)
  • Author(s): 寺内真,加藤敦之,長里千香子,本村泰三
  • Organizer: 藻類学会第33回大会
  • Place of Presentation: 琉球大学,沖縄
  • Year and Date: 20090326-20090329
• [Presentation] The function of actin on cytokinesis in brown algae, Fucus distichus and Silvetia babingtonii. (2008)
  • Author(s): Nagasato C., Motomura T.
  • Organizer: The Vth Asian Pacific Phycological Forum
  • Place of Presentation: Victoria University of Wellington, Wellington, New Zealand
  • Year and Date: 20081110-20081114
• [Presentation] Ultrastructure of nuclear and nucleomorph divisions and analysis on the cell cycle in Pyrenomonas helgolandii (Cryptophyta). (2008)
  • Author(s): Sato T., Nagasato C., Motomura T., Hara Y
  • Organizer: The Vth Asian Pacific Phycological Forum
  • Place of Presentation: Victoria University of Wellington, Wellington, New Zealand
  • Year and Date: 20081110-20081114
• [Presentation] Phylogenic and evolutionary significance of cell division in the Rhodophyceae. (2008)
  • Author(s): Kushibiki A., Yokoyama A., Nagasato C., Motomura T., Hara Y.
  • Organizer: The Vth Asian Pacific Phycological Forum
  • Place of Presentation: Victoria University of Wellington, Wellington, New Zealand
  • Year and Date: 20081110-20081114
• [Presentation] Scytosiphon lomentaria, using proteomics and differential gene expression. (2008)
  • Author(s): Klochkova T. A., Shim J. B., Han J. W., Kim G. H., Nagasato C., Motomura T.
  • Organizer: The Vth Asian Pacific Phycological Forum
  • Place of Presentation: Victoria University of Wellington, Wellington, New Zealand
  • Year and Date: 20081110-20081114
• [Presentation] 褐藻類の細胞質分裂に出現するプレート様アクチン構造の挙動 (2008)
  • Author(s): 長里千香子・本村泰三
  • Organizer: 日本植物学会第72回大会
  • Place of Presentation: 高知大学,高知
  • Year and Date: 20080924-20080927
• [Presentation] 褐藻類における異なる有性生殖様式間でのミトコンドリア細胞質遺伝機構の比較 (2008)
  • Author(s): 木村圭,長里千香子,本村泰三
  • Organizer: 日本植物学会第72回大会
  • Place of Presentation: 高知大学,高知
  • Year and Date: 20080924-20080927
• [Presentation] クリプト藻Phyrenomonas helgolandiiにおけるヌクレオモルフ分裂及びその分裂周期の解析 (2008)
  • Author(s): 佐藤友則,長里千香子,原慶明,本村泰三
  • Organizer: 日本植物学会第72回大会
  • Place of Presentation: 高知大学,高知市
  • Year and Date: 20080924-20080927
• [Presentation] Cytoplasmic inheritance of mitochondria in brown algae. (2008)
  • Author(s): Kimura K., Nagasato C., Motomura T.
  • Organizer: Ectocarpus 2008
  • Place of Presentation: SAMS Dunstaffnage Marine Laboratory, Oban, UK
  • Year and Date: 20080604-20080608
• [Presentation] Two membrane systems involved in cytokinesis of brown algae. (2008)
  • Author(s): Nagasato C, Motomura T.
  • Organizer: Ectocarpus 2008
  • Place of Presentation: SAMS Dunstaffnage Marine Laboratory, Oban, UK
  • Year and Date: 20080604-20080608
• [Presentation] 褐藻類の受精時における雄性配偶子由来ミトコンドリアDNAの消失時期の比較 (2008)
  • Author(s): 木村圭,小亀一弘,長里千香子,本村泰三
  • Organizer: 日本藻類学会第32回大会
  • Place of Presentation: 東京海洋大学,東京
  • Year and Date: 20080321-20090324
• [Presentation] 褐藻類の細胞質分裂時に現れる平板小嚢の形成と機能 (2008)
  • Author(s): 長里千香子,本村泰三
  • Organizer: 日本藻類学会第32回大会
  • Place of Presentation: 東京海洋大学,東京
  • Year and Date: 20080321-20080324
• [Presentation] クリプト藻Pyrenomonas helgolandiiにおける核分裂およびヌクレオモルフ分裂の形態学的観察 (2008)
  • Author(s): 佐藤友則,長里千香子,本村泰三,原慶明
  • Organizer: 日本藻類学会第32回大会
  • Place of Presentation: 東京海洋大学,東京
  • Year and Date: 20080321-20080324
• [Remarks] ホームページ等
  • URL: http://www.fsc.hokudai.ac.jp/muroran/