Structural study of proteasome by single particle analysis

2012 Fiscal Year Final Research Report

• Project/Area Number: 20370065
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biophysics
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: MITSUOKA Kaoru 独立行政法人産業技術総合研究所, バイオメディシナル情報研究センター, 研究チーム長 (60301230)
• Co-Investigator(Kenkyū-buntansha): YASUNAGA Takuo 九州工業大学, 大学院・情報工学研究院生命情報工学系, 教授 (60251394) ; SAEKI Yasushi 公益財団法人東京都医学総合研究所, 生体分子先端研究分野, 主席研究員 (80462779)
• Project Period (FY): 2008 – 2012
• Keywords: 構造生物学 / プロテアソーム / 単粒子解析 / 電子顕微鏡 / 三次元再構成

Research Abstract

The proteasome is a protein complex, which serves to degrade proteins, and is a protease in the major cytosolic proteolytic system with critical functions in cell cycle control, transcription, signal transduction, inflammation and many

Research Products

• [Journal Article] Obtaining high-resolution images of biological macromolecules by using a cryo-electron microscope with a liquid-helium cooled stage (2011)
  • Author(s): Mitsuoka, K
  • Journal Title: Micron Volume: vol. 42 Pages: 100-106
  • DOI: DOI:10.1016/j.micron.2010.08.006
  • Peer Reviewed
• [Journal Article] Helical image reconstruction of the outward-open human erythrocyte band 3 membrane domain in tubular crystals (2010)
  • Author(s): Yamaguchi, T., Fujii, T., Abe, T., Hirai, T., Kang, D., Namba, K., Hamasaki, N., Mitsuoka, K
  • Journal Title: J. Struct. Biol Volume: vol. 169 Pages: 406-412
  • DOI: DOI:10.1016/j.jsb.2009.12.009
  • Peer Reviewed
• [Journal Article] Multiple proteasome-interacting proteins assist the assembly of the yeast 19S regulatory particle (2009)
  • Author(s): Saeki, Y., Toh-E, A., Kudo, T., Kawamura, H., Tanaka, K
  • Journal Title: Cell Volume: vol. 137 Pages: 900-913
  • DOI: DOI:10.1016/j.cell.2009.05.005
  • Peer Reviewed
• [Journal Article] Evaluation of a 2k CCD camera with an epitaxially grown CsI scintillator for recroding envery-filtered electron cryo-micrographs (2008)
  • Author(s): Yasunaga, T., Wakabayashi, T.
  • Journal Title: J. Electron Microsc. Volume: vol. 57 Pages: 101-112
  • DOI: DOI:10.1093/jmicro/dfn006
  • Peer Reviewed
• [Presentation] Structural analysis of the 26S proteasome by cryo-electron micirscopy and single-particle analysis (2012)
  • Author(s): Wang, Z
  • Organizer: 第50回日本生物物理学会年会
  • Place of Presentation: 名古屋大学東山キャンパス(愛知県)
  • Year and Date: 2012-09-23
• [Presentation] 3D structure of the 26S proteasome by high-resolution electron cryo-microscopy (2011)
  • Author(s): Okuma, Y.
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 兵庫県立大学姫路書写キャンパス(兵庫県)
  • Year and Date: 2011-09-11
• [Presentation] Single Particle analysis of the 26S proteasome by high-resolution electron cryo-microscopy and advanced image analysis (2010)
  • Author(s): Okuma, Y
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 東北大学川内北キャンパス(福島県)
  • Year and Date: 2010-09-21
• [Book] Biological Chemistry (2012)
  • Author(s): Tanaka, K. et al
  • Total Pages: 15