2010 Fiscal Year Final Research Report
Molecular mechanisms of recovery of stalled DNA replication fork
| Project/Area Number |
20370068
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
MAKI Hisaji Nara Institute of Science and Technology, バイオサイエンス研究科, 教授 (20199649)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 遺伝学 / 突然変異 / DNA複製 |
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| Research Abstract |
Using oriC plasmid DNA replication in vitro, we analyzed molecular mechanisms recovering the stalled replication fork. We found that a translesion DNA polymerase, Pol IV, readily catalyzed a bypass DNA synthesis at a DNA lesion placed on leading or lagging strand DNA and that the replication fork progression was resumed by this bypass DNA synthesis. We demonstrated that Pol IV rapidly (<15 sec) obstructs the stable interaction between Pol III^* and the beta clamp (the lifetime of the complex >5min), causing the removal of Pol III^* from template DNA. Our study suggests a model in which the interaction between Pol III^* and the beta clamp is mediated by Pol IV to ensure that DNA replication proceeds with minimal interruption.
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