Functional analysis of the kinase complex that regulates the connection between the centrosome cycle and M phase.

2010 Fiscal Year Final Research Report

• Project/Area Number: 20370081
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: Osaka University
• Principal Investigator: NOJIMA Hiroshi Osaka University, 微生物病研究所, 教授 (30156195)
• Co-Investigator(Kenkyū-buntansha): YABUTA Norikazu 大阪大学, 微生物病研究所, 助教 (10343245) ; OKUZAKI Daisuke 大阪大学, 微生物病研究所, 助教 (00346131) ; NAITO Yoko 大阪大学, 微生物病研究所, 特任研究員 (10553026) ; KASAMA Takashi 大阪大学, 微生物病研究所, 特任研究員 (20456932)
• Project Period (FY): 2008 – 2010
• Keywords: 細胞周期 / 中心体 / 紡錘体形成チェックポイント / LATS2 / GAK / Cyclin G / PP2A B'γ / リン酸化

Research Abstract

The roles of the Lats1/2 and KBG complexes that connect centrosome cycle and M phase are analyzed. We found that Lats1/2 kinase links the signal emitted from centrosomal Aurora A to Aurora B kinases and that the he signal emitted from centrosomal Chkl kinase to CDC25, an M phase phosphatese, thereby connecting the centrosomal signal to the M phase regulatory machinery. Moreover, we showed and pointed out that the centrosomal GAK regulates PP2A B'γ that may control the phosphorylation states of certain M phase targets.

Research Products

• [Journal Article] A novel Chk1/2-Lats2-14-3-3 signaling pathway regulates P-body formation in response to UV damage. (2011)
  • Author(s): Okada N, Yabuta N, Suzuki H, Aylon Y, Oren M, Nojima H.
  • Journal Title: J.Cell Sci. 124(Pt1) Pages: 57-67
  • Peer Reviewed
• [Journal Article] a novel hollow fiber array for focused microarray analysis. (2010)
  • Author(s): Okuzaki D, Fukushima T, Tougan T, Ishii T., Kobayashi S, Yoshizaki K, Akita T, Nojima H. Genopal TM
  • Journal Title: DNA Res. 17(6) Pages: 369-79
  • Peer Reviewed
• [Journal Article] The Mek1 phosphorylation cascade plays a role in meiotic recombination of Schizosaccharomyces pombe. (2010)
  • Author(s): Tougan T, Kasama T, Ohtaka A, Okuzaki D, Saito TT, Russell P, Nojima H.
  • Journal Title: Cell Cycle. 9(23) Pages: 4688-4702
  • Peer Reviewed
• [Journal Article] Nucleoredoxin Sustains Wnt/β-Catenin Signaling by Retaining a Pool of Inactive Dishevelled Protein. (2010)
  • Author(s): Funato Y, Terabayashi T, Sakamoto R, Okuzaki D, Ichise H, Nojima H, Yoshida N, Miki H.
  • Journal Title: Curr.Biol. 20(21) Pages: 1945-1952
  • Peer Reviewed
• [Journal Article] The Lats2 tumor suppressor augments p53-mediated apoptosis by promoting the nuclear proapoptotic function of ASPP1. (2010)
  • Author(s): Aylon Y, Ofir-Rosenfeld, Y., Yabuta N, Lap, E. Nojima H, Lu, X., Oren M.
  • Journal Title: Genes Dev. 24(21) Pages: 2420-2429
  • Peer Reviewed
• [Journal Article] Spo5 phosphorylation is essential for its own timely degradation and for successful meiosis in Schizosaccharomyces pombe. (2010)
  • Author(s): Okuzaki D, Kasama T, Hirata A, Ohtaka A, Kakegawa R, Nojima H.
  • Journal Title: Cell Cycle. 9(18) Pages: 3751-3760
  • Peer Reviewed
• [Journal Article] Mug28, a Meiosis-specific Protein of Schizosaccharomyces pombe, Regulates Spore Wall Formation. (2010)
  • Author(s): Shigehisa A, Okuzaki D, Kasama T, Tohda H, Hirata A, Nojima H.
  • Journal Title: Mol Biol Cell. 21(12) Pages: 1955-1967
  • Peer Reviewed
• [Journal Article] Silencing of the Lats2 tumor suppressor overrides a p53-dependent oncogenic stress checkpoint and enables mutant H-Ras-driven cell transformation. (2009)
  • Author(s): Aylon Y, Yabuta N, Besserglick H, Buganim Y, Rotter V, Nojima H, Oren M.
  • Journal Title: Oncogene. 28(50) Pages: 4469-4479
  • Peer Reviewed
• [Journal Article] GAK, a regulator of clathrin-mediated membrane traffic, also controls centrosome integrity and chromosome congression. (2009)
  • Author(s): Shimizu H, Nagamori I, Yabuta N, Nojima H
  • Journal Title: J Cell Sci. 122(Pt17) Pages: 3145-3152
  • Peer Reviewed
• [Journal Article] GAK, a regulator of clathrin-mediated membrane trafficking, localizes not only in the cytoplasm but also in the nucleus. (2009)
  • Author(s): Sato, J., Shimizu, H., Kasama, T., Yabuta, N. Nojima, H.
  • Journal Title: Genes Cells 14 Pages: 627-641
  • Peer Reviewed
• [Journal Article] The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. (2009)
  • Author(s): Nishioka, N., Inoue, K., Adachi, K., Kiyonari, H., Ota, M., Ralston, A., Yabuta, N., Hirahara, S., Stephenson., R.O., Ogonuki, N., Makita, R., Kurihara, H., Morin-Kensicki, E.M., Nojima, H., Rossant, J., Nakao, K., Niwa, H. Sasaki, H.
  • Journal Title: Dev Cell. 16(3) Pages: 398-410
  • Peer Reviewed
• [Journal Article] Lats2 is a negative regulator of myocyte size in the heart. (2008)
  • Author(s): Matsui Y, Nakano N, Shao D, Gao S, Luo W, Hong C, Zhai P, Holle E, Yu X, Yabuta N, Tao W, Wagner T, Nojima H, Sadoshima J.
  • Journal Title: Circ Res. 103(11) Pages: 1309-1318
  • Peer Reviewed
• [Journal Article] Chum-RNA allows preparation of a high-quality cDNA library from a single-cell quantity of mRNA without PCR amplification. (2008)
  • Author(s): Tougan T, Okuzaki D, Nojima H.
  • Journal Title: Nuc.Acids Res. 36(15) Pages: e92
  • Peer Reviewed
• [Journal Article] Mug27 is a meiosis-specific protein kinase that functions in fission yeast meiosis II and sporulation. (2008)
  • Author(s): Ohtaka A, Okuzaki D, Nojima H.
  • Journal Title: J.Cell Sci. 121(9) Pages: 1547-1558
  • Peer Reviewed
• [Presentation] Lats1DN/DN MEFs expressing the N-terminal truncated Lats1 protein showed mitotic defects and formed tumors in nude mice. (2010)
  • Author(s): Nojima H, Mukai S, Suzuki H, Okada N, Miura D, Okuzaki D, Yabuta N
  • Organizer: The second workshop on the HIPPO tumor suppressor pathway
  • Place of Presentation: Rome, Italy
  • Year and Date: 2010-11-04
• [Book] Preparation of a high-quality cDNA library from a single-cell quantity of mRNA using Chum-RNA. In cDNA Librarires (Ed. By L.Chaofu, Browse J, Wallis JG). Humana Press (2011)
  • Author(s): Nojima H, Tougan T.
  • Total Pages: 15-35
  • Publisher: Springer Science
• [Remarks] ホームページ
  • URL: http://www.biken.osaka-u.ac.jp/lab/molgenet/
• [Patent(Industrial Property Rights)] 全身性エリテマトーデス患者血液細胞で発現亢進している遺伝子群、およびその利用 (2011)
  • Inventor(s): 野島博、恩田弘明、佐伯行彦
  • Industrial Property Rights Holder: 科学技術振興機構
  • Industrial Property Number: 特許,特許第4471803号
  • Acquisition Date: 2011-03-12
• [Patent(Industrial Property Rights)] ヒト腫瘍関連遺伝子とタンパク質 (2010)
  • Inventor(s): 渡邉雅文、野島博、玉井克之
  • Industrial Property Rights Holder: (株)医学生物学研究所(MBL)
  • Industrial Property Number: 特許,特許第4567140号
  • Acquisition Date: 2010-08-13
• [Patent(Industrial Property Rights)] 白血球タンパク質の回収方法および回収装置 (2009)
  • Inventor(s): 野島博、藪田紀一、奥崎大介
  • Industrial Property Rights Holder: 大阪大学
  • Industrial Property Number: 特許,K2009-0042
  • Filing Date: 2009-10-26
• [Patent(Industrial Property Rights)] コネキシン26阻害剤およびそれを用いた癌転移抑制剤 (2009)
  • Inventor(s): 野島博、伊藤彰彦、北泰行
  • Industrial Property Rights Holder: 科学技術振興機構
  • Industrial Property Number: 特許,特許第4331115号
  • Acquisition Date: 2009-06-26
• [Patent(Industrial Property Rights)] 癌転移能検査方法、および癌転移抑制薬のスクリーニング方法 (2009)
  • Inventor(s): 野島博、伊藤彰彦
  • Industrial Property Rights Holder: (株)医学生物学研究所(MBL)
  • Industrial Property Number: 特許,特許第4282163号
  • Acquisition Date: 2009-03-27
• [Patent(Industrial Property Rights)] ヒト腫瘍関連遺伝子とタンパク質 (2008)
  • Inventor(s): 野島博、伊藤彰彦
  • Industrial Property Rights Holder: 科学技術振興機構
  • Industrial Property Number: 特許,特許第4076728号
  • Acquisition Date: 2008-02-08