Live imaging analysis of axis formation in mouse embryo

2010 Fiscal Year Final Research Report

• Project/Area Number: 20370088
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: National Institute for Basic Biology
• Principal Investigator: FUJIMORI Toshihiko National Institute for Basic Biology, 初期発生研究部門, 教授 (80301274)
• Project Period (FY): 2008 – 2010
• Keywords: マウス / 軸形成 / イメージング

Research Abstract

The aim of this project was to understand early events during early mouse embryonic development, from fertilization to formation of initial axis, by the continuous imaging of developing animals. In addition to a transgenic mouse line to visualize nuclei by fluorescent proteins, lines to visualize cell membrane, and future anterior region during initial axis formation were established. Techniques to observe embryonic development throughout early stages from fertilization to axis formation have been developed. With this combination, cell behavior including cell division and migration were analyzed.

Research Products

• [Journal Article] Analysis of ciliary beat frequency and ovum transport ability in the mouse oviduct. (2011)
  • Author(s): Shi D., Komatsu K., Uemura T., Fujimori T.
  • Journal Title: Genes to Cells 16 Pages: 282-90
  • Peer Reviewed
• [Journal Article] Roles of PDGF receptor-beta in the structure and function of postnatal kidney glomerulus. (2011)
  • Author(s): Nakagawa T., Izumino K., Ishii Y., Oya T., Hamashima T., Jie S., Tomoda F., Fujimori T., Nabeshima Y., Inoue H., Sasahara M.
  • Journal Title: Nephrol Dial Transplant. 26 Pages: 458-68
  • Peer Reviewed
• [Journal Article] Preimplantation development of mouse : A view from cellular behavior. (2010)
  • Author(s): Toshihiko Fujimori
  • Journal Title: Development, Growth & Differentiation 52 Pages: 253-262
  • Peer Reviewed
• [Journal Article] Stem cell systems in development of mammals. (2010)
  • Author(s): Hitoshi Niwa, Toshihiko Fujimori
  • Journal Title: Development, Growth & Differentiation 52 Pages: 251-251
  • Peer Reviewed
• [Journal Article] Neuroprotective effects of PDGF against oxidative stress and the signaling pathway involved. (2010)
  • Author(s): Zheng L., Ishii Y., Tokunaga A., Hamashima T., Shen J., Zhao QL., Ishizawa S., Fujimroi T., Nabeshima Y., Mori H., Kondo T., Sasahara M.
  • Journal Title: J Neurosci.Res. 88 Pages: 1273-84
  • Peer Reviewed
• [Journal Article] Lack of cadherins Celsr2 and Celsr3 impairs ependymal ciliogenesis, leading to fata hydrocephalus. (2010)
  • Author(s): Fadel Tissir, Yibo Qu, Mireille Montcouquiol, Libing Zhou, Kouji Komatsu, Dongbo Shi, Toshihiko Fujimori, Jason Labeau, Donatienne Tyteca, Pierre Courtoy, Yves Poumay, Tadashi Uemura, Andre M Goffinet
  • Journal Title: Nature Neuroscience 13 Pages: 700-707
  • Peer Reviewed
• [Journal Article] Morphological organization of the mouse preimplantation embryo. (2009)
  • Author(s): Fujimori T., Kurotaki Y., Komatsu K.,Nabeshima Y.
  • Journal Title: Reprod Sci. 16 Pages: 171-7
  • Peer Reviewed
• [Journal Article] Characterization of neuroprogenitor cells expressing the PDGF beta-receptor within the subventricular zone of postnatal mice. (2008)
  • Author(s): Ishi i Y., Matsumoto Y., Watanabe R., Elmi M., Fujimori T., Nissen J., Cao Y., Nabeshima Y., Sasahara M., Funa K.
  • Journal Title: Mol Cell Neurosci 37 Pages: 507-18
  • Peer Reviewed
• [Journal Article] PDGF receptor beta is a potent regulator of mesenchymal stromal cell function. (2008)
  • Author(s): Tokunaga A., Oya T., Ishii Y., Motomura H., Nakamura C., Ishizawa S., Fujimori T., Nabeshima Y., Umezawa A., Kanamori M., Kimura T., Sasahara M.
  • Journal Title: J Bone Miner Res 23 Pages: 1519-28
  • Peer Reviewed
• [Journal Article] Deficiency of Zonula Occludens-1 Causes Embryonic Lethal Phenotype Associated with Defected Yolk Sac Angiogenesis and Apoptosis of Embryonic Cells. (2008)
  • Author(s): Katsuno T., Umeda K., Matsui T., Hata M., Tamura A., Itoh M., Takeuchi K., Fujimori T., Nabeshima Y. I., Noda T., Tsukita S.
  • Journal Title: Mol Biol Cell 19 Pages: 2465-2475
  • Peer Reviewed
• [Presentation] 藤森俊彦 Single cell level analysis of the changes of Nanog gene expression in living preimplantation mouse embryos. (2010)
  • Author(s): 藤森俊彦
  • Organizer: 第16回武田科学振興財団生命科学シンポジウム
  • Place of Presentation: Tokyo(poster)
  • Year and Date: 20101201-20101202
• [Presentation] ほ乳類初期発生の理解に向けて求めるもの (2010)
  • Author(s): 藤森俊彦
  • Organizer: 定量生物学の会第3回年会
  • Place of Presentation: Tokyo(poster)
  • Year and Date: 20101126-20101128
• [Presentation] マウス卵管上皮における膜タンパク質の局在の極性定量化 (2010)
  • Author(s): 石東博、小松紘司、平尾真由美、上村匡、藤森俊彦
  • Organizer: 定量生物学の会第3回年会
  • Place of Presentation: Tokyo(poster)
  • Year and Date: 20101126-20101128
• [Presentation] Cellular behaviors in early mammalian embryonic development (2010)
  • Author(s): 藤森俊彦
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: Sendai
  • Year and Date: 20100920-20100922
• [Presentation] マウス初期胚における細胞と遺伝子の挙動の解析 (2010)
  • Author(s): 藤森俊彦
  • Organizer: 第51回日本組織細胞化学会総会・学術集会
  • Place of Presentation: Tokyo(invited)
  • Year and Date: 20100904-20100905
• [Presentation] Analysis of Nanog expression during blastocyst formation in mouse (2010)
  • Author(s): 藤森俊彦, 藤森俊彦
  • Organizer: 第43回日本発生生物学会年会
  • Place of Presentation: Kyoto(poster)
  • Year and Date: 20100620-20100623
• [Presentation] Analysis of ciliary beat frequency and ovum transport ability in mouse oviduct (2010)
  • Author(s): Dongbo Shi, Koji Komatsu, Tadashi Uemura, Toshihiko Fujimori
  • Organizer: 第43回日本発生生物学会年会
  • Place of Presentation: Kyoto(poster)
  • Year and Date: 20100620-20100623
• [Presentation] 新たに広がる哺乳類初期発生研究の可能性 (2010)
  • Author(s): 藤森俊彦
  • Organizer: 第62回日本細胞生物学会大会
  • Place of Presentation: Osaka
  • Year and Date: 20100519-20100521
• [Presentation] Behaviors of Cell and Gene Expression during the Preimplantation Mouse Embryo (2010)
  • Author(s): 藤森俊彦
  • Organizer: The First SKLRB Symposia on Frontiers in Periimplantation Biology
  • Place of Presentation: Beijing, China(invited)
  • Year and Date: 20100508-20100512
• [Presentation] Analysis of ciliary beat frequency and ovum transport activity in mouse oviduct. (2010)
  • Author(s): Dongbo Shi, Koji Komatsu, Tadashi Uemura, Toshihiko Fujimori
  • Organizer: The First SKLRB Symposia on Frontiers in Periimplantation Biology
  • Place of Presentation: Beijing, China(poster)
  • Year and Date: 20100508-20100512
• [Presentation] マウス初期発生研究における定量化 (2010)
  • Author(s): 藤森俊彦
  • Organizer: 定量生物学の会 第2回年会
  • Place of Presentation: Osaka(poster)
  • Year and Date: 20100109-20100111
• [Presentation] マウス初期胚のライブイメージング (2009)
  • Author(s): 藤森俊彦
  • Organizer: 奈良先端科学技術大学院大学シンポジウム「視る生物学4」
  • Place of Presentation: Nara(invited)
  • Year and Date: 20091124-20091125
• [Presentation] Cell divisions relating to the morphogenesis of the early mouse embryo (2009)
  • Author(s): 藤森俊彦
  • Organizer: マウス初期発生における細胞の分裂と形態形成、第82回日本生化学会大会
  • Place of Presentation: Kobe(invited)
  • Year and Date: 20091021-20091024
• [Presentation] Cell behaviors and the differentiation of cells during preimplantation mouse development. (2009)
  • Author(s): 藤森俊彦
  • Organizer: 42^<nd> Annual Meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: Niigata, Japan(invited)
  • Year and Date: 20090528-20090531
• [Presentation] Analysis of Cell Behaviors in Early Mouse Development. (2009)
  • Author(s): 藤森俊彦
  • Organizer: 第9回NIBB-EMBL Symposium
  • Place of Presentation: Okazaki,Japan
  • Year and Date: 20090420-20090422
• [Presentation] Analysis of cell behaviors during preimplantation mouse development. Keystone Symposia (2009)
  • Author(s): 藤森俊彦
  • Organizer: Frontiers in Reproductive Biologyand Regulation of Fertility
  • Place of Presentation: Santa Fe(Invited)
  • Year and Date: 20090201-20090206
• [Presentation] Analysis of cell behaviors in early mouse embryos. (2008)
  • Author(s): 藤森俊彦
  • Organizer: SanFrancisco-Japan Joint Meeting on Vertebrate Organogenesis
  • Place of Presentation: San Francisco(invited)
  • Year and Date: 20081124-20081125
• [Presentation] Analysis of cell behaviors in early mouse embryos. (2008)
  • Author(s): 藤森俊彦
  • Organizer: Joint Symposium : Vertebrate Development and Organogenesis
  • Place of Presentation: Houston(invited)
  • Year and Date: 20081020-20081021
• [Presentation] The analysis of cell movement and expression of Oct4, Nanog and Cdx2 during mouse blastocyst formation. (2008)
  • Author(s): Kouji Komatsu, Yo-ichi Nabeshima, Toshihiko Fujimori
  • Organizer: Joint meeting of the French and Japanese Society for Developmental Biology
  • Place of Presentation: Giens peninsula,France(poster)
  • Year and Date: 20080913-20080917
• [Presentation] The expression of Cdx2 changes according to the position of cells in mouse blastocyst formation. (2008)
  • Author(s): Kouji Komatsu, Yo-ichi Nabeshima, Toshihiko Fujimori
  • Organizer: 第41回日本発生生物学会大会
  • Place of Presentation: 徳島(ポスター発表)
  • Year and Date: 20080528-20080530
• [Presentation] 胚の形作りと関連する局所的細胞周期 (2008)
  • Author(s): 藤森俊彦
  • Organizer: 日本分子生物学会第31回年会シンポジウム「細胞周期制御と高次生命現象」
  • Place of Presentation: 神戸(招待講演)
  • Year and Date: 20080000
• [Book] HORMONE FRONTIER IN GYNECOLOGY 18(ライブセルイメージングによる着床前胚発生研究 No.1) (2011)
  • Author(s): 小松紘司、藤森俊彦
  • Total Pages: 13-18
• [Book] マウス初期胚における細胞分裂と発生、細胞周期フロンティア (2010)
  • Author(s): 藤森俊彦
  • Total Pages: 168-173
  • Publisher: 共立出版
• [Book] 実験医学 26(マウス着床前胚のライブイメージング No.17) (2008)
  • Author(s): 藤森俊彦
  • Total Pages: 2778-2783
• [Remarks] ホームページ等
• [Remarks] アウトリーチ活動
• [Remarks] (1)基礎生物学研究所一般公開、体作りの始まり～卵から体ができるまで～、Oct.2, 2010(Okazaki)
• [Remarks] (2)藤森俊彦、哺乳類初期発生の理解の為のライブイメージング、第9回自然科学研究機構シンポジウム、ビックリ4Dで見るサイエンスの革新、Mar.21 2010(Tokyo)
• [Remarks] (3)藤森俊彦、基礎生物学研究所・生物学オリンピック選手合宿講義(発生学)「脊椎動物の発生」、Dec.25,2009.
• [Remarks] (4)藤森俊彦、愛知県立岡崎高等学校スーパーサイエンスハイスクール出張講義、「哺乳類の体の形作りの始まり」Dec.8,2009