Functional analysis of focal adhesion proteins-sensors and re gulators for microenvironment

2011 Fiscal Year Final Research Report

• Project/Area Number: 20380186
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: Kyoto University
• Principal Investigator: KIOKA Noriyuki 京都大学, 大学院・農学研究科, 准教授 (90234179)
• Co-Investigator(Renkei-kenkyūsha): MATSUO Michinori 京都大学, 農学研究科, 助教 (00335308)
• Project Period (FY): 2008 – 2011
• Keywords: 細胞接着 / がん / 細胞骨格 / 細胞外基質 / シグナル伝達 / 細胞・組織

Research Abstract

We have shown that vinexin, a focal adhesion protein, inhibits tumor cell migration but promotes cutaneous wound healing by controlling the localization of growth factor receptors. We have also shown that WAVE2, an actin-nucleating factor that binds to vinexin, recruits cAMP-dependent kinase to the membrane protrusion and promotes the protrusion. Furthermore, another vinexin-binding protein Dlg5 can regulate the secretion of fibronectin, a extracellular matrix. Together, these results indicate the function of focal adhesion proteins as sensors and regulators for cellular microenvironment.

Research Products

• [Journal Article] Role of Dlg5/lp-dlg, a membrane-associated guanylate kinase family protein, in epithelial-mesenchymal transition (2012)
  • Author(s): Sezaki, T., K. Inada, T. Sogabe, K. Kakuda, L. Tomiyama, Y. Matsuno, T. Ichikawa, M. Matsuo, K. Ueda, and N. Kioka
  • Journal Title: PLOS One Volume: 7 Pages: e35519
  • DOI: doi:10.1371/journal.pone.0035519
  • Peer Reviewed
• [Journal Article] WAVE2 Forms a Complex with PKA and Is Involved in PKA Enhancement of Membrane Protrusions (2011)
  • Author(s): Yamashita, H., K. Ueda, and N. Kioka
  • Journal Title: J Biol Chem Volume: 286 Pages: 3907-3914
  • DOI: doi:10.1074/jbc.M110.145409
  • Peer Reviewed
• [Journal Article] Crucial role of vinex in for keratinocyte migration in vitro and epidermal wound healing in vivo (2010)
  • Author(s): Kioka, N., T. Ito, H. Yamashita, N. Uekawa, T. Umemoto, S. Motoyoshi, H. Imai, K. Takahashi, H. Watanabe, M. Yamada, and K. Ueda
  • Journal Title: Experimental cell research Volume: 316 Pages: 1728-1738
  • URL: http://dx.doi.org/10.1016/j.yexcr.2010.03.019
  • Peer Reviewed
• [Journal Article] Src-mediated transformation suppresses the expression of focal adhesion protein vinexin (2009)
  • Author(s): Umemoto, T., T. Inomoto, K. Ueda, M. Hamaguchi, and N. Kioka
  • Journal Title: Cancer Lett Volume: 279 Pages: 22-29
  • Peer Reviewed
• [Journal Article] Tyrosine phosphorylation of vinexin in v-Src-transformed cells attenuates the affinity for vinculin (2009)
  • Author(s): Umemoto, T., K. Tanaka, K. Ueda, and N. Kioka
  • Journal Title: Biochem Biophys Res Commun Volume: 387 Pages: 191-195
  • Peer Reviewed
• [Presentation] ビネキシンはビンキュリンの活性化を促進する (2012)
  • Author(s): 市川尚文、山下寛、木村泰久、植田和光、木岡紀幸
  • Organizer: 2012年度日本農芸化学会
  • Place of Presentation: 京都
  • Year and Date: 20120322-26
• [Presentation] クローン病関連タンパク質Dlg5とTGF-βI型受容体の結合部位の同定 (2012)
  • Author(s): 瀬崎拓人、市川尚文、Lucia Tomiyama、植田和光、木岡紀幸
  • Organizer: 2012年度日本農芸化学会
  • Place of Presentation: 京都
  • Year and Date: 20120322-26
• [Presentation] 接着斑タンパク質ビンキュリンは脂質ラフトに局在する (2012)
  • Author(s): 長里彩花、山下寛、市川尚文、植田和光、木岡紀幸
  • Organizer: 2012年度日本農芸化学会
  • Place of Presentation: 京都
  • Year and Date: 20120322-26
• [Presentation] 天然変性領域を介した蛋白質間相互作用と細胞外環境の感知 (2012)
  • Author(s): 木岡紀幸
  • Organizer: 2012年度日本農芸化学会
  • Place of Presentation: 京都
  • Year and Date: 20120320-26
• [Presentation] ビンキュリンはビネキシンに依存して脂質ラフトと相互作用する (2011)
  • Author(s): 山下寛、市川尚文、長里彩花、植田和光、木岡紀幸
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 20110921-25
• [Presentation] クローン病関連タンパク質Dlg5/lp-dlgによるTGF-β受容体の分解制御 (2011)
  • Author(s): 瀬崎拓人、Lucia Tomiyama、稲田康輝、植田和光、木岡紀幸
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 20110921-24
• [Presentation] ビネキシンはビンキュリンとアクチン繊維の複合体形成を促進する (2011)
  • Author(s): 市川尚文、山下寛、木村泰久、植田和光、木岡紀幸
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 20110921-24
• [Presentation] Crohn's disease associated protein Dlg5/lp-dlg controls TGF-βsignals (2011)
  • Author(s): T. Sezaki, K. Inada, T. Sogabe, K. Ueda, N. Kioka
  • Organizer: 第63回日本細胞生物学会大会
  • Place of Presentation: 札幌
  • Year and Date: 20110627-29
• [Presentation] Hiroshi Yamashita, Takafumi Ichikawa, Kazumitsu Ueda Vinexin interaction is involved in the vinculin-mediated mechanosensing (2011)
  • Author(s): Noriyuki Kioka
  • Organizer: Gordon Research conferences on Fibronectin, Integrins & Related Molecules
  • Place of Presentation: Lucca(Barga) Italy
  • Year and Date: 20110501-06
• [Presentation] ビネキシン-ビンキュリン相互作用の生化学的解析 (2011)
  • Author(s): 市川尚文、山下寛、松山大輔、植田和光、木岡紀幸
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: 京都
  • Year and Date: 20110325-28
• [Presentation] メカノセンサーとしてのビネキシン-ビンキュリン相互作用 (2011)
  • Author(s): 山下寛、松山大輔、市川尚文、植田和光、木岡紀幸
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: 京都
  • Year and Date: 20110325-28
• [Presentation] Vine in interaction is involved in the vinculin-mediated mechanosensing (2011)
  • Author(s): Hiroshi Yamashita, Daisuke Matsuyama, Takafumi Ichikawa, Kazumitsu Ueda, Noriyuki Kioka
  • Organizer: 第63回日本細胞生物学会大会
  • Place of Presentation: 札幌
  • Year and Date: 2011-06-29
• [Presentation] Vinexin binding regulates the vinculin dynamics at adhesion sites (2010)
  • Author(s): Noriyuki Kioka
  • Organizer: Gordon Conferences "Signaling by adhesion receptor"
  • Place of Presentation: Waterville, ME, USA
  • Year and Date: 20100711-16
• [Presentation] Crohn's disease associated protein Dlg5/lp-dlg controls epithelial mesenchymal transition in LLcPK1 (2010)
  • Author(s): T. Sezaki, K. Inada, T. Sogabe, K. Ueda, N. Kioka
  • Organizer: 第8回iCeMS国際シンポジウム
  • Place of Presentation: 京都
  • Year and Date: 2010-11-09
• [Presentation] PKA forms a complex with WAVE2 and controls lamellipodia formation (2010)
  • Author(s): Hiroshi Yamashita, Kazumitsu Ueda, Noriyuki Kioka
  • Organizer: 第8回iCeMS国際シンポジウム
  • Place of Presentation: 京大
  • Year and Date: 2010-11-09
• [Presentation] 表皮細胞の運動と創傷治癒における細胞接着関連タンパク質ビネキシンの重要性 (2010)
  • Author(s): 木岡紀幸、伊東卓也、山下寛、上川奈津子、梅本勉、本吉創、今井裕、高橋健造、渡辺秀人、山田雅保、植田和光
  • Organizer: 2010年度日本農芸化学会関西支部大会
  • Place of Presentation: 近畿大学、奈良
  • Year and Date: 2010-10-03
• [Presentation] PKA Forms a Complex with WAVE2 and Controls Lamellipodia Formation (2009)
  • Author(s): Yamashita, K. Ueda, N. Kioka
  • Organizer: ASCB 49th Annual Meeting
  • Place of Presentation: San Diego
  • Year and Date: 20091205-09
• [Presentation] Crucial Role of Vinexin for Keratinocyte Migration In Vitro and Epidermal (2009)
  • Author(s): N. Kioka, T. Ito, N. Uekawa, T. Umemoto, S. Motoyoshi, H. Yamashita, H. Imai, K. Takahashi, H. Watanabe, M. Yamada, K. Ueda
  • Organizer: Wound Healing In Vivo ASCB 49th Annual Meeting
  • Place of Presentation: San Diego
  • Year and Date: 20091205-09
• [Presentation] Crohn's Disease Associated Protein Dlg5/Lp-Dlg Controls (2009)
  • Author(s): T. Sezaki, K. Inada, T. Sogabe, K. Kakuda, K. Ueda, N. Kioka
  • Organizer: Epithelial Mesenchymal Transition in Llcpk1 ASCB 49th Annual Meeting
  • Place of Presentation: San Diego
  • Year and Date: 20091205-09
• [Presentation] Crucial Role of Vinexin for Keratinocyte Migration in vitro And Epidermal (2009)
  • Author(s): N. Kioka, T. Ito, N. Uekawa, T. Umemoto, S. Motoyoshi, H. Yamashita, H. Imai, K. Takahashi, H. Watanabe, M. Yamada, K.
  • Organizer: Wound Healing in vivo 49th ASCB Annual
  • Place of Presentation: Meeting, San Diego, USA
  • Year and Date: 20091205-09
• [Remarks]
  • URL: http://www.biochemistry.kais.kyoto-u.ac.jp/http://www.biochemistry.kais.kyoto-u.ac.jp/http://www.biochemistry.kais.kyoto-u.ac.jp/