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2010 Fiscal Year Final Research Report

Analysis of functional significance of a newly discovered brain-specific transporter

Research Project

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Project/Area Number 20390044
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Medical pharmacy
Research InstitutionNagoya University (2009-2010)
Tohoku University (2008)

Principal Investigator

NAKAJIMA Akira  名古屋大学, 医学系研究科, 特任講師 (20419237)

Co-Investigator(Kenkyū-buntansha) MANO Nariyasu  東北大学, 病院, 教授 (50323035)
TOMIOKA Yoshihisa  東北大学, 大学院・薬学研究科, 教授 (00282062)
YAMAGUCHI Hiroaki  東北大学, 病院, 助教 (80400373)
Project Period (FY) 2008 – 2010
Keywordsトランスポーター / 胆汁酸 / 黒質 / in silico
Research Abstract

We have focused on a newly discovered human brain-specific transporter SLC10A4, which is specifically localized in the substantia nigra. Over-expression cell of human SLC10A4 was successfully generated using human HepG2 cell. A low SLC10A4-mediated uptake of bile acids, such as chenodeoxycholic acid and ursodeoxycholic acid, was observed in the transformant. The expression of SLC10A4 was investigated in various cultured cells using immunoblot analysis. Finally, human brain-derived TE671(medulloblastoma) cells were found that they had a stable expression of SLC10A4. It was noteworthy finding that the uptake of taurocholic acid was induced by thrombin pretreatment in the TE671 cells and the Km value was higher than that of known sodium taurocholate co-transporting polypeptides, such as ASBT and NTCP. We considered that SLC10A4 was special brain transporter, could be activated and responsible for the uptake of bile acids when blood clotting was occurred following blood vessel damage or tissue injury.

  • Research Products

    (13 results)

All 2011 2010 2009 2008

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (8 results)

  • [Journal Article] Simultaneous determination of guanidinosuccinic acid and guanidinoacetic acid in urine using high performance liquid chromatography/tandem mass spectrometry2010

    • Author(s)
      Saigusa D, Suzuki N, Takahashi M, Shiba K, Tanaka S, Abe T, Hishinuma T, Tomioka Y
    • Journal Title

      Analytica Chimica Acta

      Volume: 677 Pages: 169-75

    • Peer Reviewed
  • [Journal Article] Neurobehavioral effects of tetrabromobisphenol A, a brominated flame retardant, in mice2009

    • Author(s)
      Nakajima A, Saigusa D, Tetsu N, Yamakuni T, Tomioka Y, Hishinuma T
    • Journal Title

      Toxicol Lett

      Volume: 189 Pages: 78-83

    • Peer Reviewed
  • [Journal Article] Cysteinyl leukotrienes enhance the degranulation of bone marrow-derived mast cells through the autocrine mechanism2009

    • Author(s)
      Kaneko I, Suzuki K, Matsuo K, Kumagai H, Owada Y, Noguchi N, Hishinuma T, Ono M
    • Journal Title

      Tohoku J Exp Med

      Volume: 217 Pages: 185-91

    • Peer Reviewed
  • [Journal Article] The nutrient formula containing eicosapentaenoic acid and docosahexaenoic acid benefits the fatty acid status of patients receiving long-term enteral nutrition2009

    • Author(s)
      Munakata M, Nishikawa M, Togashi N, Nio E, Kobayashi Y, Omura K, Haginoya K, Tanaka S, Abe T, Hishinuma T, Chida N, Tsuchiya S, Onuma A
    • Journal Title

      Tohoku J Exp Med

      Volume: 217 Pages: 23-8

    • Peer Reviewed
  • [Journal Article] Simple quantification of lansoprazole and rabeprazole concentrations in human serum by liquid chromatography/tandem mass spectrometry2008

    • Author(s)
      Hishinuma T, Suzuki K, Yamaguchi H, Yamagishi H, Koike T, Ohara S, Shimosegawa T, Mano N, Goto J
    • Journal Title

      J Chromatogr B Analyt Technol Biomed Life Sci

      Volume: 870 Pages: 38-45

    • Peer Reviewed
  • [Presentation] 胆汁酸トランスポーターSLC10A4の機能解析2011

    • Author(s)
      阿部拓哉ら
    • Organizer
      日本薬学会第132年会
    • Place of Presentation
      札幌
    • Year and Date
      20110328-31
  • [Presentation] ヒト小脳由来TE671細胞におけるプロテアーゼ活性化トランスポーターの機能解析2011

    • Author(s)
      阿部拓哉ら
    • Organizer
      日本薬学会第131年会
    • Place of Presentation
      静岡
    • Year and Date
      20110328-31
  • [Presentation] LC/MS/MSを用いたOATP1B3の絶対定量と過剰発現細胞のプロテオーム解析2011

    • Author(s)
      金光祥臣ら
    • Organizer
      日本薬学会第131年会
    • Place of Presentation
      静岡
    • Year and Date
      20110328-31
  • [Presentation] HPLC/PDA法を用いた体内動態解析による抗アルツハイマー病天然薬物ノビレチンの脳内移行の証明2011

    • Author(s)
      三枝大輔ら
    • Organizer
      日本薬学会第131年会
    • Place of Presentation
      静岡
    • Year and Date
      20110328-31
  • [Presentation] ZIC-HILICカラムを用いたロイコボリン及びメトトレキサートの分離分析系の構築2010

    • Author(s)
      陣野大輔ら
    • Organizer
      第49回日本薬学会東北支部大会
    • Place of Presentation
      郡山
    • Year and Date
      2010-10-24
  • [Presentation] アコニット酸関連物質の高感度定量系の確立2010

    • Author(s)
      松本周平ら
    • Organizer
      第49回日本薬学会東北支部大会
    • Place of Presentation
      郡山
    • Year and Date
      2010-10-24
  • [Presentation] 新生児マウスにおけるtetrabromobisphenol A投与による行動変化及び脳蓄積性に対する定量的解析2009

    • Author(s)
      三枝大輔ら
    • Organizer
      日本薬学会第129年会
    • Place of Presentation
      京都
    • Year and Date
      20090326-28
  • [Presentation] Guanidinosuccininc acid及び関連化合物のLC/MS/MSを用いた定量系の確立2009

    • Author(s)
      三枝大輔ら
    • Organizer
      第48回日本薬学会東北支部大会
    • Place of Presentation
      仙台
    • Year and Date
      2009-10-18

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Published: 2013-07-31  

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